LOCUS       V025440                10996 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025440
VERSION     V025440
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             874..1590
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1642..6486
                     /label="LRP5(NM_002335)"
                     /note="LRP5(NM_002335)"
                     /gene="LRP5"
     polyA_signal    6563..6787
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6833..7261
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7275..7604
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7671..8465
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8639..8760
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8809..8825)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8833..8849
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8857..8887)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8902..8923
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9211..9796)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9967..10827)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10828..10932)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc
      901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1561 atcactctcg gcatggacga gctgtacaag ggaggcggag gtagtggagg agggggaagc
     1621 ggaggcggag ggagtgaatt catggaggca gcgccgcccg ggccgccgtg gccgctgctg
     1681 ctgctgctgc tgctgctgct ggcgctgtgc ggctgcccgg cccccgccgc ggcctcgccg
     1741 ctcctgctat ttgccaaccg ccgggacgta cggctggtgg acgccggcgg agtcaagctg
     1801 gagtccacca tcgtggtcag cggcctggag gatgcggccg cagtggactt ccagttttcc
     1861 aagggagccg tgtactggac agacgtgagc gaggaggcca tcaagcagac ctacctgaac
     1921 cagacggggg ccgccgtgca gaacgtggtc atctccggcc tggtctctcc cgacggcctc
     1981 gcctgcgact gggtgggcaa gaagctgtac tggacggact cagagaccaa ccgcatcgag
     2041 gtggccaacc tcaatggcac atcccggaag gtgctcttct ggcaggacct tgaccagccg
     2101 agggccatcg ccttggaccc cgctcacggg tacatgtact ggacagactg gggtgagacg
     2161 ccccggattg agcgggcagg gatggatggc agcacccgga agatcattgt ggactcggac
     2221 atttactggc ccaatggact gaccatcgac ctggaggagc agaagctcta ctgggctgac
     2281 gccaagctca gcttcatcca ccgtgccaac ctggacggct cgttccggca gaaggtggtg
     2341 gagggcagcc tgacgcaccc cttcgccctg acgctctccg gggacactct gtactggaca
     2401 gactggcaga cccgctccat ccatgcctgc aacaagcgca ctggggggaa gaggaaggag
     2461 atcctgagtg ccctctactc acccatggac atccaggtgc tgagccagga gcggcagcct
     2521 ttcttccaca ctcgctgtga ggaggacaat ggcggctgct cccacctgtg cctgctgtcc
     2581 ccaagcgagc ctttctacac atgcgcctgc cccacgggtg tgcagctgca ggacaacggc
     2641 aggacgtgta aggcaggagc cgaggaggtg ctgctgctgg cccggcggac ggacctacgg
     2701 aggatctcgc tggacacgcc ggacttcacc gacatcgtgc tgcaggtgga cgacatccgg
     2761 cacgccattg ccatcgacta cgacccgcta gagggctatg tctactggac agatgacgag
     2821 gtgcgggcca tccgcagggc gtacctggac gggtctgggg cgcagacgct ggtcaacacc
     2881 gagatcaacg accccgatgg catcgcggtc gactgggtgg cccgaaacct ctactggacc
     2941 gacacgggca cggaccgcat cgaggtgacg cgcctcaacg gcacctcccg caagatcctg
     3001 gtgtcggagg acctggacga gccccgagcc atcgcactgc accccgtgat gggcctcatg
     3061 tactggacag actggggaga gaaccctaaa atcgagtgtg ccaacttgga tgggcaggag
     3121 cggcgtgtgc tggtcaatgc ctccctcggg tggcccaacg gcctggccct ggacctgcag
     3181 gaggggaagc tctactgggg agacgccaag acagacaaga tcgaggtgat caatgttgat
     3241 gggacgaaga ggcggaccct cctggaggac aagctcccgc acatttttgg gttcacgctg
     3301 ctgggggact tcatctactg gactgactgg cagcgccgca gcatcgagcg ggtgcacaag
     3361 gtcaaggcca gccgggacgt catcattgac cagctgcccg acctgatggg gctcaaagct
     3421 gtgaatgtgg ccaaggtcgt cggaaccaac ccgtgtgcgg acaggaacgg ggggtgcagc
     3481 cacctgtgct tcttcacacc ccacgcaacc cggtgtggct gccccatcgg cctggagctg
     3541 ctgagtgaca tgaagacctg catcgtgcct gaggccttct tggtcttcac cagcagagcc
     3601 gccatccaca ggatctccct cgagaccaat aacaacgacg tggccatccc gctcacgggc
     3661 gtcaaggagg cctcagccct ggactttgat gtgtccaaca accacatcta ctggacagac
     3721 gtcagcctga agaccatcag ccgcgccttc atgaacggga gctcggtgga gcacgtggtg
     3781 gagtttggcc ttgactaccc cgagggcatg gccgttgact ggatgggcaa gaacctctac
     3841 tgggccgaca ctgggaccaa cagaatcgaa gtggcgcggc tggacgggca gttccggcaa
     3901 gtcctcgtgt ggagggactt ggacaacccg aggtcgctgg ccctggatcc caccaagggc
     3961 tacatctact ggaccgagtg gggcggcaag ccgaggatcg tgcgggcctt catggacggg
     4021 accaactgca tgacgctggt ggacaaggtg ggccgggcca acgacctcac cattgactac
     4081 gctgaccagc gcctctactg gaccgacctg gacaccaaca tgatcgagtc gtccaacatg
     4141 ctgggtcagg agcgggtcgt gattgccgac gatctcccgc acccgttcgg tctgacgcag
     4201 tacagcgatt atatctactg gacagactgg aatctgcaca gcattgagcg ggccgacaag
     4261 actagcggcc ggaaccgcac cctcatccag ggccacctgg acttcgtgat ggacatcctg
     4321 gtgttccact cctcccgcca ggatggcctc aatgactgta tgcacaacaa cgggcagtgt
     4381 gggcagctgt gccttgccat ccccggcggc caccgctgcg gctgcgcctc acactacacc
     4441 ctggacccca gcagccgcaa ctgcagcccg cccaccacct tcttgctgtt cagccagaaa
     4501 tctgccatca gtcggatgat cccggacgac cagcacagcc cggatctcat cctgcccctg
     4561 catggactga ggaacgtcaa agccatcgac tatgacccac tggacaagtt catctactgg
     4621 gtggatgggc gccagaacat caagcgagcc aaggacgacg ggacccagcc ctttgttttg
     4681 acctctctga gccaaggcca aaacccagac aggcagcccc acgacctcag catcgacatc
     4741 tacagccgga cactgttctg gacgtgcgag gccaccaata ccatcaacgt ccacaggctg
     4801 agcggggaag ccatgggggt ggtgctgcgt ggggaccgcg acaagcccag ggccatcgtc
     4861 gtcaacgcgg agcgagggta cctgtacttc accaacatgc aggaccgggc agccaagatc
     4921 gaacgcgcag ccctggacgg caccgagcgc gaggtcctct tcaccaccgg cctcatccgc
     4981 cctgtggccc tggtggtgga caacacactg ggcaagctgt tctgggtgga cgcggacctg
     5041 aagcgcattg agagctgtga cctgtcaggg gccaaccgcc tgaccctgga ggacgccaac
     5101 atcgtgcagc ctctgggcct gaccatcctt ggcaagcatc tctactggat cgaccgccag
     5161 cagcagatga tcgagcgtgt ggagaagacc accggggaca agcggactcg catccagggc
     5221 cgtgtcgccc acctcactgg catccatgca gtggaggaag tcagcctgga ggagttctca
     5281 gcccacccat gtgcccgtga caatggtggc tgctcccaca tctgtattgc caagggtgat
     5341 gggacaccac ggtgctcatg cccagtccac ctcgtgctcc tgcagaacct gctgacctgt
     5401 ggagagccgc ccacctgctc cccggaccag tttgcatgtg ccacagggga gatcgactgt
     5461 atccccgggg cctggcgctg tgacggcttt cccgagtgcg atgaccagag cgacgaggag
     5521 ggctgccccg tgtgctccgc cgcccagttc ccctgcgcgc ggggtcagtg tgtggacctg
     5581 cgcctgcgct gcgacggcga ggcagactgt caggaccgct cagacgaggc ggactgtgac
     5641 gccatctgcc tgcccaacca gttccggtgt gcgagcggcc agtgtgtcct catcaaacag
     5701 cagtgcgact ccttccccga ctgtatcgac ggctccgacg agctcatgtg tgaaatcacc
     5761 aagccgccct cagacgacag cccggcccac agcagtgcca tcgggcccgt cattggcatc
     5821 atcctctctc tcttcgtcat gggtggtgtc tattttgtgt gccagcgcgt ggtgtgccag
     5881 cgctatgcgg gggccaacgg gcccttcccg cacgagtatg tcagcgggac cccgcacgtg
     5941 cccctcaatt tcatagcccc gggcggttcc cagcatggcc ccttcacagg catcgcatgc
     6001 ggaaagtcca tgatgagctc cgtgagcctg atggggggcc ggggcggggt gcccctctac
     6061 gaccggaacc acgtcacagg ggcctcgtcc agcagctcgt ccagcacgaa ggccacgctg
     6121 tacccgccga tcctgaaccc gccgccctcc ccggccacgg acccctccct gtacaacatg
     6181 gacatgttct actcttcaaa cattccggcc actgcgagac cgtacaggcc ctacatcatt
     6241 cgaggaatgg cgcccccgac gacgccctgc agcaccgacg tgtgtgacag cgactacagc
     6301 gccagccgct ggaaggccag caagtactac ctggatttga actcggactc agacccctat
     6361 ccacccccac ccacgcccca cagccagtac ctgtcggcgg aggacagctg cccgccctcg
     6421 cccgccaccg agaggagcta cttccatctc ttcccgcccc ctccgtcccc ctgcacggac
     6481 tcatcctgag gatcctgata tccagcacag tggcggccgc tcgagtctag agggcccgtt
     6541 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6601 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6661 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6721 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6781 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6841 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6901 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6961 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7021 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7081 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7141 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7201 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7261 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7321 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7381 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7441 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7501 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7561 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     7621 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     7681 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7741 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7801 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     7861 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     7921 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     7981 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     8041 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     8101 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     8161 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     8221 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     8281 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     8341 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     8401 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     8461 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     8521 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     8581 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     8641 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8701 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8761 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     8821 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8881 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8941 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9001 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9061 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9721 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     9781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    10021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    10081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    10141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    10201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    10261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10861 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10921 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
    10981 cccgatcccc tatggt
//
