LOCUS V021924 6495 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021924 VERSION V021924 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 898..924 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 928..954 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 958..984 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1018..1983 /label="KMT5A(NM_001324504)" /note="KMT5A(NM_001324504)" /gene="KMT5A" promoter complement(2047..2065) /note="promoter for bacteriophage SP6 RNA polymerase" /label="SP6 promoter" polyA_signal 2091..2315 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2361..2789 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2803..3133 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3200..3994 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4168..4289 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4338..4354) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4362..4378 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4386..4416) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4431..4452 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4740..5328) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5499..6359) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6360..6464) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccgc caccatgtac 901 ccatacgacg tcccagacta cgctacttat ccttatgatg tacctgatta tgctagctat 961 ccttatgatg tcccagacta cgcatcagct ggaagcttgg taccgagctc ggatccaatg 1021 gctagaggca ggaagatgtc caagccccgc accgacgggg agaacgtatt taccgggcag 1081 tcaaagatct attcctacat gagcccgaac aaatgctctg gaatgcgttt cccccttcag 1141 gaagagaact cagttacaca tcacgaagtc aaatgccagg ggaaaccatt agccggaatc 1201 tacaggaaac gagaagagaa aagaaatgct gggaacgcag tacggagcgc catgaagtcc 1261 gaggaacaga agatcaaaga cgccaggaaa ggtcccctgg taccttttcc aaaccaaaaa 1321 tctgaagcag cagaacctcc aaaaactcca ccctcatctt gtgattccac caatgcagcc 1381 atcgccaagc aagccctgaa aaagcccatc aagggcaaac aggccccccg aaaaaaagct 1441 caaggaaaaa cgcaacagaa tcgcaaactt acggatttct accctgtccg aaggagctcc 1501 aggaagagca aagccgagct gcagtctgaa gaaaggaaaa gaatagatga attgattgaa 1561 agtgggaagg aagaaggaat gaagattgac ctcatcgatg gcaaaggcag gggtgtgatt 1621 gccaccaagc agttctcccg gggtgacttt gtggtggaat accacgggga cctcatcgag 1681 atcaccgacg ccaagaaacg ggaggctctg tacgcacagg acccttccac gggctgctac 1741 atgtactatt ttcagtatct gagcaaaacc tactgcgtgg atgcaactag agagacaaat 1801 cgcctaggaa gactgatcaa tcacagcaaa tgtgggaact gccaaaccaa actgcacgac 1861 atcgacggcg tacctcacct catcctcatc gcctcccgag acatcgcggc tggggaggag 1921 ctcctgtatg actatgggga ccgcagcaag gcttccattg aagcccaccc gtggctgaag 1981 cattaagaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg 2041 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc 2101 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 2161 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 2221 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 2281 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 2341 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 2401 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 2461 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat 2521 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 2581 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 2641 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 2701 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga 2761 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 2821 ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc 2881 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 2941 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 3001 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 3061 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 3121 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac 3181 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 3241 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 3301 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 3361 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 3421 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 3481 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 3541 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 3601 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 3661 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 3721 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 3781 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 3841 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 3901 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 3961 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 4021 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 4081 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 4141 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 4201 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 4261 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 4321 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 4381 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 4441 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 4501 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 4561 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 4621 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 4681 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 4741 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 4801 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 4861 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 4921 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 4981 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 5041 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 5101 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 5161 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 5221 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 5281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 5341 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 5401 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 5461 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 5521 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 5581 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 5641 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 5701 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 5761 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 5821 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 5881 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 5941 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 6001 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 6061 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 6121 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 6181 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 6241 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 6301 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 6361 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 6421 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 6481 gtgccacctg acgtc //