LOCUS V019282 7904 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019282 VERSION V019282 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..3436 /label="KAT2B(NM_003884)" /note="KAT2B(NM_003884)" /gene="KAT2B" CDS 3443..3466 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" polyA_signal 3504..3728 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3774..4202 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4216..4545 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4612..5406 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5580..5701 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5750..5766) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5774..5790 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5798..5828) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5843..5864 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6152..6737) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6908..7768) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7769..7873) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgtccgagg ctggcggggc 961 cgggccgggc ggctgcgggg caggagccgg ggcaggggcc gggcccgggg cgctgccccc 1021 gcagcctgcg gcgcttccgc ccgcgccccc gcagggctcc ccctgcgccg ctgccgccgg 1081 gggctcgggc gcctgcggtc cggcgacggc agtggctgca gcgggcacgg ccgaaggacc 1141 gggaggcggt ggctcggccc gaatcgccgt gaagaaagcg caactacgct ccgctccgcg 1201 ggccaagaaa ctggagaaac tcggagtgta ctccgcctgc aaggccgagg agtcttgtaa 1261 atgtaatggc tggaaaaacc ctaacccctc acccactccc cccagagccg acctgcagca 1321 aataattgtc agtctaacag aatcctgtcg gagttgtagc catgccctag ctgctcatgt 1381 ttcccacctg gagaatgtgt cagaggaaga aatgaacaga ctcctgggaa tagtattgga 1441 tgtggaatat ctctttacct gtgtccacaa ggaagaagat gcagatacca aacaagttta 1501 tttctatcta tttaagctct tgagaaagtc tattttacaa agaggaaaac ctgtggttga 1561 aggctctttg gaaaagaaac ccccatttga aaaacctagc attgaacagg gtgtgaataa 1621 ctttgtgcag tacaaattta gtcacctgcc agcaaaagaa aggcaaacaa tagttgagtt 1681 ggcaaaaatg ttcctaaacc gcatcaacta ttggcatctg gaggcaccat ctcaacgaag 1741 actgcgatct cccaatgatg atatttctgg atacaaagag aactacacaa ggtggctgtg 1801 ttactgcaac gtgccacagt tctgcgacag tctacctcgg tacgaaacca cacaggtgtt 1861 tgggagaaca ttgcttcgct cggtcttcac tgttatgagg cgacaactcc tggaacaagc 1921 aagacaggaa aaagataaac tgcctcttga aaaacgaact ctaatcctca ctcatttccc 1981 aaaatttctg tccatgctag aagaagaagt atatagtcaa aactctccca tctgggatca 2041 ggattttctc tcagcctctt ccagaaccag ccagctaggc atccaaacag ttatcaatcc 2101 acctcctgtg gctgggacaa tttcatacaa ttcaacctca tcttcccttg agcagccaaa 2161 cgcagggagc agcagtcctg cctgcaaagc ctcttctgga cttgaggcaa acccaggaga 2221 aaagaggaaa atgactgatt ctcatgttct ggaggaggcc aagaaacccc gagttatggg 2281 ggatattccg atggaattaa tcaacgaggt tatgtctacc atcacggacc ctgcagcaat 2341 gcttggacca gagaccaatt ttctgtcagc acactcggcc agggatgagg cggcaaggtt 2401 ggaagagcgc aggggtgtaa ttgaatttca cgtggttggc aattccctca accagaaacc 2461 aaacaagaag atcctgatgt ggctggttgg cctacagaac gttttctccc accagctgcc 2521 ccgaatgcca aaagaataca tcacacggct cgtctttgac ccgaaacaca aaacccttgc 2581 tttaattaaa gatggccgtg ttattggtgg tatctgtttc cgtatgttcc catctcaagg 2641 attcacagag attgtcttct gtgctgtaac ctcaaatgag caagtcaagg gctatggaac 2701 acacctgatg aatcatttga aagaatatca cataaagcat gacatcctga acttcctcac 2761 atatgcagat gaatatgcaa ttggatactt taagaaacag ggtttctcca aagaaattaa 2821 aatacctaaa accaaatatg ttggctatat caaggattat gaaggagcca ctttaatggg 2881 atgtgagcta aatccacgga tcccgtacac agaattttct gtcatcatta aaaagcagaa 2941 ggagataatt aaaaaactga ttgaaagaaa acaggcacaa attcgaaaag tttaccctgg 3001 actttcatgt tttaaagatg gagttcgaca gattcctata gaaagcattc ctggaattag 3061 agagacaggc tggaaaccga gtggaaaaga gaaaagtaaa gagcccagag accctgacca 3121 gctttacagc acgctcaaga gcatcctcca gcaggtgaag agccatcaaa gcgcttggcc 3181 cttcatggaa cctgtgaaga gaacagaagc tccaggatat tatgaagtta taaggttccc 3241 catggatctg aaaaccatga gtgaacgcct caagaatagg tactacgtgt ctaagaaatt 3301 attcatggca gacttacagc gagtctttac caattgcaaa gagtacaacc cccctgagag 3361 tgaatactac aaatgtgcca atatcctgga gaaattcttc ttcagtaaaa ttaaggaagc 3421 tggattaatt gacaagctcg aggattacaa ggatgacgac gataagtagt gagggcccgt 3481 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 3541 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 3601 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 3661 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 3721 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 3781 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 3841 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 3901 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 3961 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 4021 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 4081 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 4141 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 4201 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 4261 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 4321 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 4381 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 4441 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 4501 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 4561 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa 4621 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 4681 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 4741 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag 4801 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 4861 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 4921 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 4981 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 5041 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 5101 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat 5161 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 5221 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 5281 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 5341 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 5401 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 5461 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 5521 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 5581 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 5641 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 5701 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 5761 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 5821 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 5881 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 5941 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 6001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 6061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 6121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 6181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 6301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 6361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 6481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 6541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 6601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 6661 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca 6721 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 6781 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 6841 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 6901 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 6961 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 7021 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 7081 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7141 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 7201 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 7261 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 7321 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 7381 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 7441 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 7501 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 7561 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 7621 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 7681 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 7741 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 7801 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 7861 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc //