LOCUS       V020509                 8699 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020509
VERSION     V020509
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3499
                     /label="Il17ra(NM_008359)"
                     /note="Il17ra(NM_008359)"
                     /gene="Il17ra"
     CDS             3515..4222
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    4266..4490
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4536..4964
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4978..5307
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5374..6168
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6342..6463
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6512..6528)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6536..6552
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6560..6590)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6605..6626
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6914..7499)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7670..8530)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8531..8635)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcgattcggc gctgctggcc acgggtcgtc cccgggcccg cgctgggatg
      961 gctgcttctg ctgctgaacg ttctggcccc gggccgcgcc tccccgcgcc tcctcgactt
     1021 cccggctccg gtctgcgcgc aggaggggct gagctgcaga gtcaagaata gtacttgtct
     1081 ggatgacagc tggatccacc ccaaaaacct gaccccgtct tccccaaaaa acatctatat
     1141 caatcttagt gtttcctcta cccagcacgg agaattagtc cctgtgttgc atgttgagtg
     1201 gaccctgcag acagatgcca gcatcctgta cctcgagggt gcagagctgt ccgtcctgca
     1261 gctgaacacc aatgagcggc tgtgtgtcaa gttccagttt ctgtccatgc tgcagcatca
     1321 ccgtaagcgg tggcggtttt ccttcagcca ctttgtggta gatcctggcc aggagtatga
     1381 agtgactgtt caccacctgc cgaagcccat ccctgatggg gacccaaacc acaaatccaa
     1441 gatcatcttt gtgcctgact gtgaggacag caagatgaag atgactacct catgcgtgag
     1501 ctcaggcagc ctttgggatc ccaacatcac tgtggagacc ttggacacac agcatctgcg
     1561 agtggacttc accctgtgga atgaatccac cccctaccag gtcctgctgg aaagtttctc
     1621 cgactcagag aaccacagct gctttgatgt cgttaaacaa atatttgcgc ccaggcaaga
     1681 agaattccat cagcgagcta atgtcacatt cactctaagc aagtttcact ggtgctgcca
     1741 tcaccacgtg caggtccagc ccttcttcag cagctgccta aatgactgtt tgagacacgc
     1801 tgtgactgtg ccctgcccag taatctcaaa taccacagtt cccaagccag ttgcagacta
     1861 cattcccctg tgggtgtatg gcctcatcac actcatcgcc attctgctgg tgggatctgt
     1921 catcgtgctg atcatctgta tgacctggag gctttctggc gccgatcaag agaaacatgg
     1981 tgatgactcc aaaatcaatg gcatcttgcc cgtagcagac ctgactcccc cacccctgag
     2041 gcccaggaag gtctggatcg tctactcggc cgaccacccc ctctatgtgg aggtggtcct
     2101 aaagttcgcc cagttcctga tcactgcctg tggcactgaa gtagcccttg acctcctgga
     2161 agagcaggtt atctctgagg tgggggtcat gacctgggtg agccgacaga agcaggagat
     2221 ggtggagagc aactccaaaa tcatcatcct gtgttcccga ggcacccaag caaagtggaa
     2281 agctatcttg ggttgggctg agcctgctgt ccagctacgg tgtgaccact ggaagcctgc
     2341 tggggacctt ttcactgcag ccatgaacat gatcctgcca gacttcaaga ggccagcctg
     2401 cttcggcacc tacgttgttt gctacttcag tggcatctgt agtgagaggg atgtccccga
     2461 cctcttcaac atcacctcca ggtacccact catggacaga tttgaggagg tttacttccg
     2521 gatccaggac ctggagatgt ttgaacccgg ccggatgcac catgtcagag agctcacagg
     2581 ggacaattac ctgcagagcc ctagtggccg gcagctcaag gaggctgtgc ttaggttcca
     2641 ggagtggcaa acccagtgcc ccgactggtt cgagcgtgag aacctctgct tagctgatgg
     2701 ccaagatctt ccctccctgg atgaagaagt gtttgaagac ccactgctgc caccaggggg
     2761 aggaattgtc aaacagcagc ccctggtgcg ggaactccca tctgacggct gccttgtggt
     2821 agatgtctgt gtcagtgagg aagaaagtag aatggcaaag ctggaccctc agctatggcc
     2881 acagagagag ctagtggctc acaccctcca aagcatggtg ctgccagcag agcaggtccc
     2941 tgcagctcat gtggtggagc ctctccatct cccagacggc agtggagcag ctgcccagct
     3001 gcccatgaca gaggacagcg aggcttgccc gctgctgggg gtccagagga acagcatcct
     3061 ttgcctcccc gtggactcag atgacttgcc actctgtagc accccaatga tgtcacctga
     3121 ccacctccaa ggcgatgcaa gagagcagct agaaagccta atgctctcgg tgctgcagca
     3181 gagcctgagt ggacagcccc tggagagctg gccgaggcca gaggtggtcc tcgagggctg
     3241 cacaccctct gaggaggagc agcggcagtc ggtgcagtcg gaccagggct acatctccag
     3301 gagctccccg cagccccccg agtggctcac ggaggaggaa gagctagaac tgggtgagcc
     3361 cgttgagtct ctctctcctg aggaactacg gagcctgagg aagctccaga ggcagctttt
     3421 cttctgggag ctcgagaaga accctggctg gaacagcttg gagccacgga gacccacccc
     3481 agaagagcag aatccctccg gaggaggtgg cagcgtgagc aagggcgagg aggataacat
     3541 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     3601 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3661 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     3721 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     3781 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     3841 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     3901 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3961 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     4021 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     4081 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     4141 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     4201 catggacgag ctgtacaagt gactcgagtc tagagggccc gtttaaaccc gctgatcagc
     4261 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4321 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4381 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4441 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4501 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4561 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4621 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4681 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4741 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4801 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4861 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4921 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4981 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5041 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     5101 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     5161 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5221 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     5281 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5341 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5401 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5461 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5521 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5581 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5641 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5701 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5761 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5821 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5881 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5941 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6001 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6061 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6121 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     6181 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     6241 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6301 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6481 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6541 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6601 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6961 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7021 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7081 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7141 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7201 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7261 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7321 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7381 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7441 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7621 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7681 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7741 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7801 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7861 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7921 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7981 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8041 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8101 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8161 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8221 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8281 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8341 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8401 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8461 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8521 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8581 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8641 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
