LOCUS V022588 7844 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022588 VERSION V022588 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..3334 /label="ITGB7(NM_000889)" /note="ITGB7(NM_000889)" /gene="ITGB7" CDS 3341..3406 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3444..3668 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3714..4142 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4156..4485 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4552..5346 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5520..5641 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5690..5706) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5714..5730 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5738..5768) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5783..5804 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6092..6677) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6848..7708) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7709..7813) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggtggctt tgccaatggt 961 ccttgttttg ctgctggtcc tgagcagagg tgagagtgaa ttggacgcca agatcccatc 1021 cacaggggat gccacagaat ggcggaatcc tcacctgtcc atgctggggt cctgccagcc 1081 agccccctcc tgccagaagt gcatcctctc acaccccagc tgtgcatggt gcaagcaact 1141 gaacttcacc gcgtcgggag aggcggaggc gcggcgctgc gcccgacgag aggagctgct 1201 ggctcgaggc tgcccgctgg aggagctgga ggagccccgc ggccagcagg aggtgctgca 1261 ggaccagccg ctcagccagg gcgcccgcgg agagggtgcc acccagctgg cgccgcagcg 1321 ggtccgggtc acgctgcggc ctggggagcc ccagcagctc caggtccgct tccttcgtgc 1381 tgagggatac ccggtggacc tgtactacct tatggacctg agctactcca tgaaggacga 1441 cctggaacgc gtgcgccagc tcgggcacgc tctgctggtc cggctgcagg aagtcaccca 1501 ttctgtgcgc attggttttg gttcctttgt ggacaaaacg gtgctgccct ttgtgagcac 1561 agtaccctcc aaactgcgcc acccctgccc cacccggctg gagcgctgcc agtcaccatt 1621 cagctttcac catgtgctgt ccctgacggg ggacgcacaa gccttcgagc gggaggtggg 1681 gcgccagagt gtgtccggca atctggactc gcctgaaggt ggcttcgatg ccattctgca 1741 ggctgcactc tgccaggagc agattggctg gagaaatgtg tcccggctgc tggtgttcac 1801 ttcagacgac acattccata cagctgggga cgggaagttg ggcggcattt tcatgcccag 1861 tgatgggcac tgccacttgg acagcaatgg cctctacagt cgcagcacag agtttgacta 1921 cccttctgtg ggtcaggtag cccaggccct ctctgcagca aatatccagc ccatctttgc 1981 tgtcaccagt gccgcactgc ctgtctacca ggagctgagt aaactgattc ctaagtctgc 2041 agttggggag ctgagtgagg actccagcaa cgtggtacag ctcatcatgg atgcttataa 2101 tagcctgtct tccaccgtga cccttgaaca ctcttcactc cctcctgggg tccacatttc 2161 ttacgaatcc cagtgtgagg gtcctgagaa gagggagggt aaggctgagg atcgaggaca 2221 gtgcaaccac gtccgaatca accagacggt gactttctgg gtttctctcc aagccaccca 2281 ctgcctccca gagccccatc tcctgaggct ccgggccctt ggcttctcag aggagctgat 2341 tgtggagttg cacacgctgt gtgactgtaa ttgcagtgac acccagcccc aggctcccca 2401 ctgcagtgat ggccagggac acctacaatg tggtgtatgc agctgtgccc ctggccgcct 2461 aggtcggctc tgtgagtgct ctgtggcaga gctgtcctcc ccagacctgg aatctgggtg 2521 ccgggctccc aatggcacag ggcccctgtg cagtggaaag ggtcactgtc aatgtggacg 2581 ctgcagctgc agtggacaga gctctgggca tctgtgcgag tgtgacgatg ccagctgtga 2641 gcgacatgag ggcatcctct gcggaggctt tggtcgctgc caatgtggag tatgtcactg 2701 tcatgccaac cgcacgggca gagcatgcga atgcagtggg gacatggaca gttgcatcag 2761 tcccgaggga gggctctgca gtgggcatgg acgctgcaaa tgcaaccgct gccagtgctt 2821 ggacggctac tatggtgctc tatgcgacca atgcccaggc tgcaagacac catgcgagag 2881 acaccgggac tgtgcagagt gtggggcctt caggactggc ccactggcca ccaactgcag 2941 tacagcttgt gcccatacca atgtgaccct ggccttggcc cctatcttgg atgatggctg 3001 gtgcaaagag cggaccctgg acaaccagct gttcttcttc ttggtggagg atgacgccag 3061 aggcacggtc gtgctcagag tgagacccca agaaaaggga gcagaccaca cgcaggccat 3121 tgtgctgggc tgcgtagggg gcatcgtggc agtggggctg gggctggtcc tggcttaccg 3181 gctctcggtg gaaatctatg accgccggga atacagtcgc tttgagaagg agcagcaaca 3241 actcaactgg aagcaggaca gtaatcctct ctacaaaagt gccatcacga ccaccatcaa 3301 tcctcgcttt caagaggcag acagtcccac tctcctcgag gactacaaag accatgacgg 3361 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt gagggcccgt 3421 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 3481 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 3541 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 3601 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 3661 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 3721 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 3781 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 3841 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 3901 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 3961 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 4021 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 4081 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 4141 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 4201 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 4261 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 4321 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 4381 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 4441 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 4501 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa 4561 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 4621 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 4681 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag 4741 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 4801 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 4861 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 4921 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 4981 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 5041 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat 5101 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 5161 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 5221 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 5281 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 5341 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 5401 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 5461 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 5521 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 5581 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 5641 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 5701 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 5761 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 5821 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 5881 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 5941 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 6001 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 6061 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 6121 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6181 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 6241 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 6301 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6361 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 6421 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 6481 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 6541 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 6601 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca 6661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 6721 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 6781 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 6841 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 6901 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 6961 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 7021 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7081 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 7141 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 7201 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 7261 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 7321 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 7381 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 7441 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 7501 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 7561 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 7621 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 7681 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 7741 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 7801 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc //