LOCUS       V036040                 7949 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036040
VERSION     V036040
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3469
                     /label="HSP90AA1(NM_001017963)"
                     /note="HSP90AA1(NM_001017963)"
                     /gene="HSP90AA1"
     polyA_signal    3516..3740
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3786..4214
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4228..4557
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4624..5418
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5592..5713
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5762..5778)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5786..5802
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5810..5840)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5855..5876
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6164..6749)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6920..7780)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7781..7885)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg cccccgtgtt cgggcgggga cggctccacc cctcctgggc cctcccttcg
      961 ggacagggac tgtcccgccc agagtgctga atacccgcgc gaccgtctgg atccccgccc
     1021 aggaagcccc tctgaagcct cctcgccgcc gtttctgaga agcagggcac ctgttaactg
     1081 gtaccaagaa aaggcccaag tgtttctctg gcatctgatg gtgtctggat ccaccactct
     1141 actctgtctc tggaaacagc ccttccacgt ctctgcattc cctgtcaccg cgtcactggc
     1201 cttcagacag agccaaggtg cagggcaaca cctctacaag gatctgcagc catttatatt
     1261 gcttaggcta ctgatgcctg aggaaaccca gacccaagac caaccgatgg aggaggagga
     1321 ggttgagacg ttcgcctttc aggcagaaat tgcccagttg atgtcattga tcatcaatac
     1381 tttctactcg aacaaagaga tctttctgag agagctcatt tcaaattcat cagatgcatt
     1441 ggacaaaatc cggtatgaaa gcttgacaga tcccagtaaa ttagactctg ggaaagagct
     1501 gcatattaac cttataccga acaaacaaga tcgaactctc actattgtgg atactggaat
     1561 tggaatgacc aaggctgact tgatcaataa ccttggtact atcgccaagt ctgggaccaa
     1621 agcgttcatg gaagctttgc aggctggtgc agatatctct atgattggcc agttcggtgt
     1681 tggtttttat tctgcttatt tggttgctga gaaagtaact gtgatcacca aacataacga
     1741 tgatgagcag tacgcttggg agtcctcagc agggggatca ttcacagtga ggacagacac
     1801 aggtgaacct atgggtcgtg gaacaaaagt tatcctacac ctgaaagaag accaaactga
     1861 gtacttggag gaacgaagaa taaaggagat tgtgaagaaa cattctcagt ttattggata
     1921 tcccattact ctttttgtgg agaaggaacg tgataaagaa gtaagcgatg atgaggctga
     1981 agaaaaggaa gacaaagaag aagaaaaaga aaaagaagag aaagagtcgg aagacaaacc
     2041 tgaaattgaa gatgttggtt ctgatgagga agaagaaaag aaggatggtg acaagaagaa
     2101 gaagaagaag attaaggaaa agtacatcga tcaagaagag ctcaacaaaa caaagcccat
     2161 ctggaccaga aatcccgacg atattactaa tgaggagtac ggagaattct ataagagctt
     2221 gaccaatgac tgggaagatc acttggcagt gaagcatttt tcagttgaag gacagttgga
     2281 attcagagcc cttctatttg tcccacgacg tgctcctttt gatctgtttg aaaacagaaa
     2341 gaaaaagaac aacatcaaat tgtatgtacg cagagttttc atcatggata actgtgagga
     2401 gctaatccct gaatatctga acttcattag aggggtggta gactcggagg atctccctct
     2461 aaacatatcc cgtgagatgt tgcaacaaag caaaattttg aaagttatca ggaagaattt
     2521 ggtcaaaaaa tgcttagaac tctttactga actggcggaa gataaagaga actacaagaa
     2581 attctatgag cagttctcta aaaacataaa gcttggaata cacgaagact ctcaaaatcg
     2641 gaagaagctt tcagagctgt taaggtacta cacatctgcc tctggtgatg agatggtttc
     2701 tctcaaggac tactgcacca gaatgaagga gaaccagaaa catatctatt atatcacagg
     2761 tgagaccaag gaccaggtag ctaactcagc ctttgtggaa cgtcttcgga aacatggctt
     2821 agaagtgatc tatatgattg agcccattga tgagtactgt gtccaacagc tgaaggaatt
     2881 tgaggggaag actttagtgt cagtcaccaa agaaggcctg gaacttccag aggatgaaga
     2941 agagaaaaag aagcaggaag agaaaaaaac aaagtttgag aacctctgca aaatcatgaa
     3001 agacatattg gagaaaaaag ttgaaaaggt ggttgtgtca aaccgattgg tgacatctcc
     3061 atgctgtatt gtcacaagca catatggctg gacagcaaac atggagagaa tcatgaaagc
     3121 tcaagcccta agagacaact caacaatggg ttacatggca gcaaagaaac acctggagat
     3181 aaaccctgac cattccatta ttgagacctt aaggcaaaag gcagaggctg ataagaacga
     3241 caagtctgtg aaggatctgg tcatcttgct ttatgaaact gcgctcctgt cttctggctt
     3301 cagtctggaa gatccccaga cacatgctaa caggatctac aggatgatca aacttggtct
     3361 gggtattgat gaagatgacc ctactgctga tgataccagt gctgctgtaa ctgaagaaat
     3421 gccacccctt gaaggagatg acgacacatc acgcatggaa gaagtagact aactcgagtc
     3481 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     3541 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     3601 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3661 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     3721 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     3781 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3901 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3961 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4021 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4081 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4141 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4201 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4261 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4321 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4381 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4441 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     4501 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4561 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     4621 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     4681 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     4741 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     4801 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     4861 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     4921 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     4981 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5041 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5101 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     5161 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5221 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5281 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5341 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5401 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     5461 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5521 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5581 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5641 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5701 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     5761 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5821 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5881 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
     6721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     7921 gatcgggaga tctcccgatc ccctatggt
//
