LOCUS V018358 7926 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018358 VERSION V018358 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 922..939 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" CDS 940..3414 /label="HNRNPU(NM_031844)" /note="HNRNPU(NM_031844)" /gene="HNRNPU" promoter complement(3478..3496) /note="promoter for bacteriophage SP6 RNA polymerase" /label="SP6 promoter" polyA_signal 3522..3746 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3792..4220 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4234..4564 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4631..5425 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5599..5720 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5769..5785) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5793..5809 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5817..5847) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5862..5883 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6171..6759) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6930..7790) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7791..7895) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccgccaccat gcatcaccat caccatcaca tgagttcctc gcctgttaat 961 gtaaaaaagc tgaaggtgtc ggagctgaaa gaggagctca agaagcgacg cctttctgac 1021 aagggtctca aggccgagct catggagcga ctccaggctg cgctggacga cgaggaggcc 1081 gggggccgcc ccgccatgga gcccgggaac ggcagcctag acctgggcgg ggattccgct 1141 gggcgctcgg gagcaggcct cgagcaggag gccgcggccg gcggcgatga agaggaggag 1201 gaagaggaag aggaggagga aggaatctcc gctctggacg gcgaccagat ggagctagga 1261 gaggagaacg gggccgcggg ggcggccgac tcgggcccga tggaggagga ggaggccgcc 1321 tcggaagacg agaacggcga cgatcagggt ttccaggaag gggaagatga gctcggggac 1381 gaagaggaag gcgcgggcga cgagaacggg cacggggagc agcagcctca accgccggcg 1441 acgcagcagc aacagcccca acagcagcgc ggggccgcca aggaggccgc ggggaagagc 1501 agcggcccca cctcgctgtt cgcggtgacg gtggcgccgc ccggggcgag gcagggccag 1561 cagcaggcgg gaggtaagaa gaaggcggaa ggcggcggag gcggcggtcg ccccggggct 1621 ccggcggcgg gggacggcaa aacagaacag aaaggcggag ataaaaagag gggtgttaaa 1681 agaccacgag aagatcatgg ccgtggatat tttgagtaca ttgaagagaa caagtatagc 1741 agagccaaat ctcctcagcc acctgttgaa gaagaagatg aacacttcga tgacacagtg 1801 gtttgtcttg atacttataa ttgtgatcta cattttaaaa tatcaagaga tcgtctcagt 1861 gcttcttccc ttacaatgga gagttttgct tttctttggg ctggaggaag agcatcctat 1921 ggtgtgtcaa aaggcaaagt gtgttttgag atgaaggtta cagagaagat cccagtaagg 1981 catttatata caaaagatat tgacatacat gaagttcgta ttggctggtc actaactaca 2041 agtggaatgt tacttggtga agaagaattt tcttatgggt attctctaaa aggaataaaa 2101 acatgcaact gtgagactga agattatgga gaaaagtttg atgaaaatga tgtgattaca 2161 tgttttgcta actttgaaag tgatgaagta gaactctcgt atgctaagaa tggacaagat 2221 cttggcgttg ccttcaaaat cagtaaggaa gttcttgctg gacggccact gttcccgcat 2281 gttctctgcc acaactgtgc agttgaattt aattttggtc agaaggaaaa gccatatttt 2341 ccaatacctg aagagtatac tttcatccag aacgtcccct tagaggatcg agttagagga 2401 ccaaaggggc ctgaagagaa gaaagattgt gaagttgtga tgatgattgg cttgccagga 2461 gctggaaaaa ctacctgggt tactaaacat gcagcagaaa atccagggaa atataacatt 2521 cttggcacaa atactattat ggataagatg atggtggcag gttttaagaa gcaaatggca 2581 gatactggaa aactgaacac actgttgcag agagcccccc agtgtcttgg gaaatttatt 2641 gagattgctg cccgaaagaa gcgaaatttt attctggatc agacaaatgt gtctgctgct 2701 gcccagagga gaaaaatgtg cctgtttgca ggcttccagc gaaaagctgt tgtagtttgc 2761 ccaaaagatg aagactataa gcaaagaaca cagaagaaag cagaagtaga ggggaaagac 2821 ctaccagaac atgcggtcct caaaatgaaa ggaaacttta ccctcccaga ggtagctgag 2881 tgctttgatg aaataaccta tgttgaactt cagaaggaag aagcccaaaa actcttggag 2941 caatataagg aagaaagcaa aaaggctctt ccaccagaaa agaaacagaa cactggctca 3001 aagaaaagca ataaaaataa gagtggcaag aaccagttta acagaggtgg tggccataga 3061 ggacgtggag gattcaatat gcgtggtgga aatttcagag gaggagcccc tgggaatcgt 3121 ggcggatata ataggagggg caacatgcca cagagaggtg gtggcggtgg aggaagtggt 3181 ggaatcggct atccataccc tcgtgcccct gtttttcctg gccgtggtag ttactcaaac 3241 agagggaact acaacagagg tggaatgccc aacagaggga actacaacca gaacttcaga 3301 ggacgaggaa acaatcgtgg ctacaaaaat caatctcagg gctacaacca gtggcagcag 3361 ggtcaattct ggggtcagaa gccatggagt cagcattatc accaaggata ttattgagaa 3421 ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg gccctattct 3481 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca 3541 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 3601 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 3661 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 3721 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 3781 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 3841 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 3901 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg 3961 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 4021 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 4081 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 4141 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta 4201 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 4261 ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 4321 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 4381 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 4441 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 4501 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 4561 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 4621 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 4681 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 4741 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 4801 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 4861 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 4921 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 4981 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 5041 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 5101 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 5161 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 5221 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 5281 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 5341 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 5401 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 5461 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 5521 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 5581 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 5641 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 5701 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 5761 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 5821 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 5881 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 5941 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 6001 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 6061 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 6121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 6181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 6241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 6301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 6361 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 6421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 6481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 6541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 6601 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 6661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 6721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 6781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 6841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 6901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 6961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 7021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 7081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 7141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 7201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 7261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 7321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 7381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 7441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 7501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 7561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 7621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 7681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 7741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 7801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 7861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 7921 gacgtc //