LOCUS       V039185                 8297 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039185
VERSION     V039185
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             884..1591
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1628..3817
                     /label="HNRNPM(NM_005968)"
                     /note="HNRNPM(NM_005968)"
                     /gene="HNRNPM"
     polyA_signal    3864..4088
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4134..4562
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4576..4905
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4972..5766
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5940..6061
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6110..6126)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6134..6150
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6158..6188)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6203..6224
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6512..7097)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7268..8128)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8129..8233)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgct accggtcgcc accatggtga gcaagggcga
      901 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      961 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     1021 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     1081 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     1141 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     1201 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     1261 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     1321 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     1381 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     1441 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     1501 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     1561 ctccaccggc ggcatggacg agctgtacaa gggtggaggc ggatcaggag gcggtggatc
     1621 tctcgagatg gcggcagggg tcgaagcggc ggcggaggtg gcggcgacgg agatcaaaat
     1681 ggaggaagag agcggcgcgc ccggcgtgcc gagcggcaac ggggctccgg gccctaaggg
     1741 tgaaggagaa cgacctgctc agaatgagaa gaggaaggag aaaaacataa aaagaggagg
     1801 caatcgcttt gagccatatg ccaatccaac taaaagatac agagccttca ttacaaacat
     1861 accttttgat gtgaaatggc agtcacttaa agacctggtt aaagaaaaag ttggtgaggt
     1921 aacatacgtg gagctcttaa tggacgctga aggaaagtca aggggatgtg ctgttgttga
     1981 attcaagatg gaagagagca tgaaaaaagc tgcggaagtc ctaaacaagc atagtctgag
     2041 cggaagacca ctgaaagtca aagaagatcc tgatggtgaa catgccagga gagcaatgca
     2101 aaaggtgatg gctacgactg gtgggatggg tatgggacca ggtggcccag gaatgattac
     2161 tatcccaccc agtatcctaa ataatcccaa catcccaaat gagattatcc atgcattaca
     2221 ggctggaaga cttggaagca cagtatttgt agcaaatctg gattataaag ttggctggaa
     2281 gaaactgaag gaagtattta gtatggctgg tgtggtggtc cgagcagaca ttcttgaaga
     2341 taaagatgga aaaagtcgtg gaataggcac tgttactttt gaacagtcca ttgaagctgt
     2401 gcaagctata tctatgttca atggccagct gctatttgat agaccaatgc acgtcaagat
     2461 ggatgagagg gccttaccaa aaggagattt cttccctcct gagcgtccac aacaacttcc
     2521 ccatggcctt ggtggtattg gcatggggtt aggaccagga gggcaaccca ttgatgccaa
     2581 tcacctgaat aaaggcatcg gaatgggaaa cataggtccc gcaggaatgg gaatggaagg
     2641 cataggattt ggaataaata aaatgggagg aatggagggg ccctttggtg gtggtatgga
     2701 aaacatgggt cgatttggat ctgggatgaa catgggcagg ataaatgaaa tcctaagtaa
     2761 tgcactgaag agaggagaga tcattgcaaa gcagggagga ggtggaggtg gaggaagcgt
     2821 ccctgggatc gagaggatgg gtcctggcat tgaccgcctc gggggtgccg gcatggagcg
     2881 catgggcgcg ggcctgggcc acggcatgga tcgcgtgggc tccgagatcg agcgcatggg
     2941 cctggtcatg gaccgcatgg gctccgtgga gcgcatgggc tccggcattg agcgcatggg
     3001 cccgctgggc ctcgaccaca tggcctccag cattgagcgc atgggccaga ccatggagcg
     3061 cattggctct ggcgtggagc gcatgggtgc cggcatgggc ttcggccttg agcgcatggc
     3121 cgctcccatc gaccgtgtgg gccagaccat tgagcgcatg ggctctggcg tggagcgcat
     3181 gggccctgcc atcgagcgca tgggcctgag catggagcgc atggtgcccg caggtatggg
     3241 agctggcctg gagcgcatgg gccccgtgat ggatcgcatg gccaccggcc tggagcgcat
     3301 gggcgccaac aatctggagc ggatgggcct ggagcgcatg ggcgccaaca gcctcgagcg
     3361 catgggcctg gagcgcatgg gtgccaacag cctcgagcgc atgggccctg ccatgggccc
     3421 ggccctgggc gctggcattg agcgcatggg cctggccatg ggtggcggtg gcggtgccag
     3481 ctttgaccgt gccatcgaga tggagcgtgg caacttcgga ggaagcttcg caggttcctt
     3541 tggtggagct ggaggccatg ctcctggggt ggccaggaag gcctgccaga tatttgtgag
     3601 aaatctgcca ttcgatttca catggaagat gctaaaggac aaattcaacg agtgcggcca
     3661 cgtgctgtac gccgacatca agatggagaa tgggaagtcc aaggggtgtg gcgtggttaa
     3721 gttcgagtcg ccagaggtgg ccgagagagc ctgccggatg atgaatggca tgaagctgag
     3781 tggccgagag attgacgttc gaattgatag aaacgcttaa ggatcctcta gagggcccgt
     3841 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3901 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3961 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4021 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4081 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4141 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4201 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4261 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4321 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4381 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4441 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4501 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4561 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4621 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4681 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4741 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4801 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     4861 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     4921 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     4981 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5041 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5101 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5161 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5221 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5281 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5341 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5401 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5461 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5521 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5581 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5641 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5701 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5761 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5821 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5881 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6061 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6301 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7021 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     7081 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7141 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7201 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7261 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7321 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7381 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7441 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7501 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7561 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7621 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7681 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7741 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7801 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7861 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7921 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7981 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8041 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8101 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8161 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8221 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8281 tcccgatccc ctatggt
//
