LOCUS       V031674                 8509 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031674
VERSION     V031674
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4002
                     /label="HERC5(NM_016323)"
                     /note="HERC5(NM_016323)"
                     /gene="HERC5"
     CDS             4009..4038
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    4076..4300
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4346..4774
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4788..5117
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5184..5978
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6152..6273
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6322..6338)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6346..6362
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6370..6400)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6415..6436
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6724..7309)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7480..8340)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8341..8445)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggagcgga ggtcgcggag gaagtcgcgg
      961 cgcaacgggc gctcgaccgc gggcaaggcc gccgcgaccc agcccgcgaa gtctccgggc
     1021 gcacagctct ggctctttcc cagcgccgcg ggcctccacc gcgcgctgct ccggagggtg
     1081 gaggtgacgc gccaactctg ctgctcgccg gggcgcctcg cggtcttgga acgcggcggg
     1141 gcgggcgtcc aggttcacca gctgctcgcc gggagcggcg gcgcccggac gccgaaatgc
     1201 attaaattag gaaaaaacat gaagatacat tccgtggacc aaggagcaga gcacatgctg
     1261 attctctcat cagatggaaa accatttgag tatgacaact atagcatgaa acatctaagg
     1321 tttgaaagca ttttacaaga aaaaaaaata attcagatca catgtggaga ttaccattct
     1381 cttgcactct caaaaggtgg tgagcttttt gcctggggac agaacctgca tgggcagctt
     1441 ggagttggaa ggaaatttcc ctcaaccacc acaccacaga ttgtggagca cctcgcagga
     1501 gtacccttgg ctcagatttc tgccggagaa gcccacagca tggccttatc catgtctggc
     1561 aacatttatt catggggaaa aaatgaatgt ggacaactag gcctgggcca cactgagagt
     1621 aaagatgatc catcccttat tgaaggacta gacaatcaga aagttgaatt tgtcgcttgt
     1681 ggtggctctc acagtgccct actcacacag gatgggctgc tgtttacttt cggtgctgga
     1741 aaacatgggc aacttggtca taattcaaca cagaatgagc taagaccctg tttggtggct
     1801 gagcttgttg ggtatagagt gactcagata gcatgtggaa ggtggcacac acttgcctat
     1861 gtttctgatt tgggaaaggt cttttccttt ggttctggaa aagatggaca actgggaaat
     1921 ggtggaacac gtgaccagct gatgccgctt ccagtgaaag tatcatcaag tgaagaactc
     1981 aaacttgaaa gccatacctc agaaaaggag ttaataatga ttgctggagg gaatcaaagc
     2041 attttgctct ggataaagaa agagaattca tatgttaatc tgaagaggac aattcctact
     2101 ctgaatgaag ggactgtaaa gagatggatt gctgatgtgg agactaaacg gtggcagagc
     2161 acaaaaaggg aaatccaaga gatattttca tctcctgctt gtctaactgg aagtttttta
     2221 aggaaaagaa gaactacaga aatgatgcct gtttatttgg acttaaataa agcaagaaac
     2281 atcttcaagg agttaaccca aaaggactgg attactaaca tgataaccac ctgcctcaaa
     2341 gataatctgc tcaaaagact tccatttcat tctccacccc aagaagcttt agaaattttc
     2401 ttccttctcc cagaatgtcc tatgatgcat atttccaaca actgggagag ccttgtggtt
     2461 ccatttgcaa aggttgtttg taaaatgagt gaccagtctt cactggttct ggaagagtat
     2521 tgggcaactc tgcaagaatc cactttcagc aaactggtcc agatgtttaa aacagccgtc
     2581 atatgccagt tggattactg ggatgaaagt gctgaggaga atggtaatgt tcaagctctc
     2641 ctagaaatgt tgaagaagct gcacagggta aaccaggtga aatgtcaact acctgaaagt
     2701 attttccaag tagacgaact cttgcaccgt ctcaattttt ttgtagaagt atgcagaagg
     2761 tacttgtgga aaatgactgt ggacgcttca gaaaatgtac aatgctgcgt catattcagt
     2821 cactttccat ttatctttaa taatctgtcg aaaattaaac tactacatac agacacactt
     2881 ttaaaaatag agagtaaaaa acataaagct tatcttaggt cggcagcaat tgaggaagaa
     2941 agagagtctg aattcgcttt gaggcccacg tttgatctaa cagtcagaag gaatcacttg
     3001 attgaggatg ttttgaatca gctaagtcaa tttgagaatg aagacctgag gaaagagtta
     3061 tgggtttcat ttagtggaga aattgggtat gacctcggag gagtcaagaa agagttcttc
     3121 tactgtctgt ttgcagagat gatccagccg gaatatggga tgttcatgta tcctgaaggg
     3181 gcttcctgca tgtggtttcc tgtcaagcct aaatttgaga agaaaagata cttctttttt
     3241 ggggttctat gtggactttc cctgttcaat tgcaatgttg ccaaccttcc tttcccactg
     3301 gcactgttta agaaactttt ggaccaaatg ccatcattgg aagacttgaa agaactcagt
     3361 cctgatttgg gaaagaattt gcaaacactt ctggatgatg aaggtgataa ctttgaggaa
     3421 gtattttaca tccattttaa tgtgcactgg gacagaaacg acacaaactt aattcctaat
     3481 ggaagtagca taactgtcaa ccagactaac aagagagact atgtttctaa gtatatcaat
     3541 tacattttca acgactctgt aaaggcggtt tatgaagaat ttcggagagg attttataaa
     3601 atgtgcgacg aagacattat caaattattc caccccgaag aactgaagga tgtgattgtt
     3661 ggaaatacag attatgattg gaaaacattt gaaaagaatg cacgttatga accaggatat
     3721 aacagttcac atcccaccat agtgatgttt tggaaggctt tccacaaatt gactctggaa
     3781 gaaaagaaaa aattccttgt atttcttaca ggaactgaca gactacaaat gaaagattta
     3841 aataatatga aaataacatt ttgctgtcct gaaagttgga atgaaagaga ccctataaga
     3901 gcactgacat gtttcagtgt cctcttcctc cctaaatatt ctacaatgga aacagttgaa
     3961 gaagcgcttc aagaagccat caacaacaac agaggatttg gcctcgagga gcagaagctg
     4021 atctcagagg aggacctgta gtgagggccc gtttaaaccc gctgatcagc ctcgactgtg
     4081 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     4141 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     4201 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     4261 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     4321 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4381 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4441 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4501 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4561 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4621 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4681 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4741 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4801 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4861 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4921 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4981 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5041 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5101 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     5161 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     5221 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5281 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5341 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     5401 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5461 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5521 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5581 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5641 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5701 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5761 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5821 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5881 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5941 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     6001 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     6061 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6121 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6241 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6301 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6361 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6421 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6481 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6661 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6721 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6781 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6901 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6961 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7021 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7081 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7141 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7201 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7261 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7321 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7441 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7501 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7561 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7621 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7681 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7741 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7801 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7861 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7921 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7981 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8041 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8101 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8161 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8221 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8281 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8341 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8401 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8461 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
