LOCUS       V031515                 9146 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031515
VERSION     V031515
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             890..4576
                     /label="HDAC6(NM_001321225)"
                     /note="HDAC6(NM_001321225)"
                     /gene="HDAC6"
     CDS             4592..4618
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4622..4648
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4652..4678
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4713..4937
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4983..5411
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5425..5754
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5821..6615
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6789..6910
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6959..6975)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6983..6999
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7007..7037)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7052..7073
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7361..7946)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8117..8977)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8978..9082)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca tgagtggagc
      901 gaaccgcggc aggggccaag cctcctcaac tatgacctca accggccagg attccaccac
      961 aaccaggcag cgaagaagta ggcagaaccc ccagtcgccc cctcaggact ccagtgtcac
     1021 ttcgaagcga aatattaaaa agggagccgt tccccgctct atccccaatc tagcggaggt
     1081 aaagaagaaa ggcaaaatga agaagctcgg ccaagcaatg gaagaagacc taatcgtggg
     1141 actgcaaggg atggatctga accttgaggc tgaagcactg gctggcactg gcttggtgtt
     1201 ggatgagcag ttaaatgaat tccattgcct ctgggatgac agcttcccgg aaggccctga
     1261 gcggctccat gccatcaagg agcaactgat ccaggagggc ctcctagatc gctgcgtgtc
     1321 ctttcaggcc cggtttgctg aaaaggaaga gctgatgttg gttcacagcc tagaatatat
     1381 tgatctgatg gaaacaaccc agtacatgaa tgagggagaa ctccgtgtcc tagcagacac
     1441 ctacgactca gtttatctgc atccgaactc atactcctgt gcctgcctgg cctcaggctc
     1501 tgtcctcagg ctggtggatg cggtcctggg ggctgagatc cggaatggca tggccatcat
     1561 taggcctcct ggacatcacg cccagcacag tcttatggat ggctattgca tgttcaacca
     1621 cgtggctgtg gcagcccgct atgctcaaca gaaacaccgc atccggaggg tccttatcgt
     1681 agattgggat gtgcaccacg gtcaaggaac acagttcacc ttcgaccagg accccagtgt
     1741 cctctatttc tccatccacc gctacgagca gggtaggttc tggccccacc tgaaggcctc
     1801 taactggtcc accacaggtt tcggccaagg ccaaggatat accatcaatg tgccttggaa
     1861 ccaggtgggg atgcgggatg ctgactacat tgctgctttc ctgcacgtcc tgctgccagt
     1921 cgccctcgag ttccagcctc agctggtcct ggtggctgct ggatttgatg ccctgcaagg
     1981 ggaccccaag ggtgagatgg ccgccactcc ggcagggttc gcccagctaa cccacctgct
     2041 catgggtctg gcaggaggca agctgatcct gtctctggag ggtggctaca acctccgcgc
     2101 cctggctgaa ggcgtcagtg cttcgctcca cacccttctg ggagaccctt gccccatgct
     2161 ggagtcacct ggtgccccct gccggagtgc ccaggcttca gtttcctgtg ctctggaagc
     2221 ccttgagccc ttctgggagg ttcttgtgag atcaactgag accgtggaga gggacaacat
     2281 ggaggaggac aatgtagagg agagcgagga ggaaggaccc tgggagcccc ctgtgctccc
     2341 aatcctgaca tggccagtgc tacagtctcg cacagggctg gtctatgacc aaaatatgat
     2401 gaatcactgc aacttgtggg acagccacca ccctgaggta ccccagcgca tcttgcggat
     2461 catgtgccgt ctggaggagc tgggccttgc cgggcgctgc ctcaccctga caccgcgccc
     2521 tgccacagag gctgagctgc tcacctgtca cagtgctgag tacgtgggtc atctccgggc
     2581 cacagagaaa atgaaaaccc gggagctgca ccgtgagagt tccaactttg actccatcta
     2641 tatctgcccc agtaccttcg cctgtgcaca gcttgccact ggcgctgcct gccgcctggt
     2701 ggaggctgtg ctctcaggag aggttctgaa tggtgctgct gtggtgcgtc ccccaggaca
     2761 ccacgcagag caggatgcag cttgcggttt ttgctttttc aactctgtgg ctgtggctgc
     2821 tcgccatgcc cagactatca gtgggcatgc cctacggatc ctgattgtgg attgggatgt
     2881 ccaccacggt aatggaactc agcacatgtt tgaggatgac cccagtgtgc tatatgtgtc
     2941 cctgcaccgc tatgatcatg gcaccttctt ccccatgggg gatgagggtg ccagcagcca
     3001 gatcggccgg gctgcgggca caggcttcac cgtcaacgtg gcatggaacg ggccccgcat
     3061 gggtgatgct gactacctag ctgcctggca tcgcctggtg cttcccattg cctacgagtt
     3121 taacccagaa ctggtgctgg tctcagctgg ctttgatgct gcacgggggg atccgctggg
     3181 gggctgccag gtgtcacctg agggttatgc ccacctcacc cacctgctga tgggccttgc
     3241 cagtggccgc attatcctta tcctagaggg tggctataac ctgacatcca tctcagagtc
     3301 catggctgcc tgcactcgct ccctccttgg agacccacca cccctgctga ccctgccacg
     3361 gcccccacta tcaggggccc tggcctcaat cactgagacc atccaagtcc atcgcagata
     3421 ctggcgcagc ttacgggtca tgaaggtaga agacagagaa ggaccctcca gttctaagtt
     3481 ggtcaccaag aaggcacccc aaccagccaa acctaggtta gctgagcgga tgaccacacg
     3541 agaaaagaag gttctggaag caggcatggg gaaagtcacc tcggcatcat ttggggaaga
     3601 gtccactcca ggccagacta actcagagac agctgtggtg gccctcactc aggaccagcc
     3661 ctcagaggca gccacagggg gagccactct ggcccagacc atttctgagg cagccattgg
     3721 gggagccatg ctgggccaga ccacctcaga ggaggctgtc gggggagcca ctccggacca
     3781 gaccacctca gaggagactg tgggaggagc cattctggac cagaccacct cagaggatgc
     3841 tgttggggga gccacgctgg gccagactac ctcagaggag gctgtaggag gagctacact
     3901 ggcccagacc acctcggagg cagccatgga gggagccaca ctggaccaga ctacgtcaga
     3961 ggaggctcca gggggcaccg agctgatcca aactcctcta gcctcgagca cagaccacca
     4021 gaccccccca acctcacctg tgcagggaac tacaccccag atatctccca gtacactgat
     4081 tgggagtctc aggaccttgg agctaggcag cgaatctcag ggggcctcag aatctcaggc
     4141 cccaggagag gagaacctac taggagaggc agctggaggt caggacatgg ctgattcgat
     4201 gctgatgcag ggatctaggg gcctcactga tcaggccata ttttatgctg tgacaccact
     4261 gccctggtgt ccccatttgg tggcagtatg ccccatacct gcagcaggcc tagacgtgac
     4321 ccaaccttgt ggggactgtg gaacaatcca agagaattgg gtgtgtctct cttgctatca
     4381 ggtctactgt ggtcgttaca tcaatggcca catgctccaa caccatggaa attctggaca
     4441 cccgctggtc ctcagctaca tcgacctgtc agcctggtgt tactactgtc aggcctatgt
     4501 ccaccaccag gctctcctag atgtgaagaa catcgcccac cagaacaagt ttggggagga
     4561 tatgccccac ccacacatgc ggccgctcga gtacccatac gacgtacctg attatgcaac
     4621 ttatccttat gatgtacctg attatgctag ctatccttat gatgtcccag actacgcatg
     4681 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4741 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4801 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4861 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4921 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     4981 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5041 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5101 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5161 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5221 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5281 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5341 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5401 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5461 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5521 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5581 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5641 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5701 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     5761 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     5821 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5881 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5941 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6001 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6061 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6121 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6181 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6241 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6301 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6361 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6421 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6481 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6541 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6601 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     6661 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6721 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6781 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6841 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6901 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6961 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7021 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7081 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7141 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7201 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7261 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7321 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7381 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7441 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7501 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7561 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7621 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7681 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7741 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7801 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7861 gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga
     7921 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7981 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8041 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8101 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8161 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8221 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8281 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8341 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8401 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8461 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8521 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8581 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8641 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8701 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8761 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8821 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8881 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8941 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9001 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9061 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9121 cgggagatct cccgatcccc tatggt
//
