LOCUS       V037466                 8834 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037466
VERSION     V037466
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4273
                     /label="HDAC5(NM_001015053)"
                     /note="HDAC5(NM_001015053)"
                     /gene="HDAC5"
     CDS             4280..4306
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4310..4336
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4340..4366
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4401..4625
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4671..5099
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5113..5442
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5509..6303
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6477..6598
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6647..6663)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6671..6687
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6695..6725)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6740..6761
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7049..7634)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7805..8665)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8666..8770)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg aactctccca acgagtcgga tgggatgtca ggtcgggaac catccttgga
      961 aatcctgccg cggacttctc tgcacagcat ccctgtgaca gtggaggtga agccggtgct
     1021 gccaagagcc atgcccagtt ccatgggggg tgggggtgga ggcagcccca gccctgtgga
     1081 gctacggggg gctctggtgg gctctgtgga ccccacactg cgggagcagc aactgcagca
     1141 ggagctcctg gcgctcaagc agcagcagca gctgcagaag cagctcctgt tcgctgagtt
     1201 ccagaaacag catgaccacc tgacaaggca gcatgaggtc cagctgcaga agcacctcaa
     1261 gcagcagcag gagatgctgg cagccaagca gcagcaggag atgctggcag ccaagcggca
     1321 gcaggagctg gagcagcagc ggcagcggga gcagcagcgg caggaagagc tggagaagca
     1381 gcggctggag cagcagctgc tcatcctgcg gaacaaggag aagagcaaag agagtgccat
     1441 tgccagcact gaggtaaagc tgaggctcca ggaattcctc ttgtcgaagt caaaggagcc
     1501 cacaccaggc ggcctcaacc attccctccc acagcacccc aaatgctggg gagcccacca
     1561 tgcttctttg gaccagagtt cccctcccca gagcggcccc cctgggacgc ctccctccta
     1621 caaactgcct ttgcctgggc cctacgacag tcgagacgac ttccccctcc gcaaaacagc
     1681 ctctgaaccc aacttgaaag tgcgttcaag gctaaaacag aaggtggctg agcggagaag
     1741 cagtcccctc ctgcgtcgca aggatgggac tgttattagc acctttaaga agagagctgt
     1801 tgagatcaca ggtgccgggc ctggggcgtc gtccgtgtgt aacagcgcac ccggctccgg
     1861 ccccagctct cccaacagct cccacagcac catcgctgag aatggcttta ctggctcagt
     1921 ccccaacatc cccactgaga tgctccctca gcaccgagcc ctccctctgg acagctcccc
     1981 caaccagttc agcctctaca cgtctccttc tctgcccaac atctccctag ggctgcaggc
     2041 cacggtcact gtcaccaact cacacctcac tgcctccccg aagctgtcga cacagcagga
     2101 ggccgagagg caggccctcc agtccctgcg gcagggtggc acgctgaccg gcaagttcat
     2161 gagcacatcc tctattcctg gctgcctgct gggcgtggca ctggagggcg acgggagccc
     2221 ccacgggcat gcctccctgc tgcagcatgt gctgttgctg gagcaggccc ggcagcagag
     2281 caccctcatt gctgtgccac tccacgggca gtccccacta gtgacgggtg aacgtgtggc
     2341 caccagcatg cggacggtag gcaagctccc gcggcatcgg cccctgagcc gcactcagtc
     2401 ctcaccgctg ccgcagagtc cccaggccct gcagcagctg gtcatgcaac aacagcacca
     2461 gcagttcctg gagaagcaga agcagcagca gctacagctg ggcaagatcc tcaccaagac
     2521 aggggagctg cccaggcagc ccaccaccca ccctgaggag acagaggagg agctgacgga
     2581 gcagcaggag gtcttgctgg gggagggagc cctgaccatg ccccgggagg gctccacaga
     2641 gagtgagagc acacaggaag acctggagga ggaggacgag gaagacgatg gggaggagga
     2701 ggaggattgc atccaggtta aggacgagga gggcgagagt ggtgctgagg aggggcccga
     2761 cttggaggag cctggtgctg gatacaaaaa actgttctca gatgcccagc cgctgcagcc
     2821 tttgcaggtg taccaggcgc ccctcagcct ggccactgtg ccccaccagg ccctgggccg
     2881 tacccagtcc tcccctgctg cccctggggg catgaagagc cccccagacc agcccgtcaa
     2941 gcacctcttc accacaggtg tggtctacga cacgttcatg ctaaagcacc agtgcatgtg
     3001 cgggaacaca cacgtgcacc ctgagcatgc tggccggatc cagagcatct ggtcccggct
     3061 gcaggagaca ggcctgctta gcaagtgcga gcggatccga ggtcgcaaag ccacgctaga
     3121 tgagatccag acagtgcact ctgaatacca caccctgctc tatgggacca gtcccctcaa
     3181 ccggcagaag ctagacagca agaagttgct cggccccatc agccagaaga tgtatgctgt
     3241 gctgccttgt gggggcatcg gggtggacag tgacaccgtg tggaatgaga tgcactcctc
     3301 cagtgctgtg cgcatggcag tgggctgcct gctggagctg gccttcaagg tggctgcagg
     3361 agagctcaag aatggatttg ccatcatccg gcccccagga caccacgccg aggaatccac
     3421 agccatggga ttctgcttct tcaactctgt agccatcacc gcaaaactcc tacagcagaa
     3481 gttgaacgtg ggcaaggtcc tcatcgtgga ctgggacatt caccatggca atggcaccca
     3541 gcaggcgttc tacaatgacc cctctgtgct ctacatctct ctgcatcgct atgacaacgg
     3601 gaacttcttt ccaggctctg gggctcctga agaggttggt ggaggaccag gcgtggggta
     3661 caatgtgaac gtggcatgga caggaggtgt ggaccccccc attggagacg tggagtacct
     3721 tacagccttc aggacagtgg tgatgcccat tgcccacgag ttctcacctg atgtggtcct
     3781 agtctccgcc gggtttgatg ctgttgaagg acatctgtct cctctgggtg gctactctgt
     3841 caccgccaga tgttttggcc acttgaccag gcagctgatg accctggcag ggggccgggt
     3901 ggtgctggcc ctggagggag gccatgactt gaccgccatc tgtgatgcct ctgaggcttg
     3961 tgtctcggct ctgctcagtg tagagctgca gcccttggat gaggcagtct tgcagcaaaa
     4021 gcccaacatc aacgcagtgg ccacgctaga gaaagtcatc gagatccaga gcaaacactg
     4081 gagctgtgtg cagaagttcg ccgctggtct gggccggtcc ctgcgagagg cccaagcagg
     4141 tgagaccgag gaggccgaga ctgtgagcgc catggccttg ctgtcggtgg gggccgagca
     4201 ggcccaggct gcggcagccc gggaacacag ccccaggccg gcagaggagc ccatggagca
     4261 ggagcctgcc ctgctcgagt acccatacga cgtacctgat tatgcaactt atccttatga
     4321 tgtacctgat tatgctagct atccttatga tgtcccagac tacgcatgag ggcccgttta
     4381 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4441 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4501 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4561 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4621 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4681 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4741 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4801 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4861 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4921 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4981 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5041 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5101 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5161 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5221 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5281 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5341 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5401 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5461 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5521 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5581 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5641 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     5701 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5761 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5821 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5881 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5941 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6001 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     6061 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6121 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6181 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6241 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6301 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6361 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6421 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6481 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6541 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6601 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6661 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6721 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6781 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6841 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6901 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6961 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7021 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7081 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7261 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7381 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7441 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7501 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7561 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7861 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7921 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7981 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8041 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8101 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8161 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8221 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8281 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8341 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8401 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8461 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8521 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8581 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8641 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8701 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8761 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     8821 cgatccccta tggt
//
