LOCUS       V035952                 9653 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035952
VERSION     V035952
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             874..1590
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1660..5025
                     /label="HDAC5(NM_001015053)"
                     /note="HDAC5(NM_001015053)"
                     /gene="HDAC5"
     CDS             5032..5058
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5062..5088
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5092..5118
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    5220..5444
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5490..5918
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5932..6261
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6328..7122
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7296..7417
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7466..7482)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7490..7506
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7514..7544)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7559..7580
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7868..8453)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8624..9484)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9485..9589)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc
      901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1561 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagg cggcggaggg
     1621 agtggaggag ggggaagcgg aggcggaggg agtaagctta tgaactctcc caacgagtcg
     1681 gatgggatgt caggtcggga accatccttg gaaatcctgc cgcggacttc tctgcacagc
     1741 atccctgtga cagtggaggt gaagccggtg ctgccaagag ccatgcccag ttccatgggg
     1801 ggtgggggtg gaggcagccc cagccctgtg gagctacggg gggctctggt gggctctgtg
     1861 gaccccacac tgcgggagca gcaactgcag caggagctcc tggcgctcaa gcagcagcag
     1921 cagctgcaga agcagctcct gttcgctgag ttccagaaac agcatgacca cctgacaagg
     1981 cagcatgagg tccagctgca gaagcacctc aagcagcagc aggagatgct ggcagccaag
     2041 cagcagcagg agatgctggc agccaagcgg cagcaggagc tggagcagca gcggcagcgg
     2101 gagcagcagc ggcaggaaga gctggagaag cagcggctgg agcagcagct gctcatcctg
     2161 cggaacaagg agaagagcaa agagagtgcc attgccagca ctgaggtaaa gctgaggctc
     2221 caggaattcc tcttgtcgaa gtcaaaggag cccacaccag gcggcctcaa ccattccctc
     2281 ccacagcacc ccaaatgctg gggagcccac catgcttctt tggaccagag ttcccctccc
     2341 cagagcggcc cccctgggac gcctccctcc tacaaactgc ctttgcctgg gccctacgac
     2401 agtcgagacg acttccccct ccgcaaaaca gcctctgaac ccaacttgaa agtgcgttca
     2461 aggctaaaac agaaggtggc tgagcggaga agcagtcccc tcctgcgtcg caaggatggg
     2521 actgttatta gcacctttaa gaagagagct gttgagatca caggtgccgg gcctggggcg
     2581 tcgtccgtgt gtaacagcgc acccggctcc ggccccagct ctcccaacag ctcccacagc
     2641 accatcgctg agaatggctt tactggctca gtccccaaca tccccactga gatgctccct
     2701 cagcaccgag ccctccctct ggacagctcc cccaaccagt tcagcctcta cacgtctcct
     2761 tctctgccca acatctccct agggctgcag gccacggtca ctgtcaccaa ctcacacctc
     2821 actgcctccc cgaagctgtc gacacagcag gaggccgaga ggcaggccct ccagtccctg
     2881 cggcagggtg gcacgctgac cggcaagttc atgagcacat cctctattcc tggctgcctg
     2941 ctgggcgtgg cactggaggg cgacgggagc ccccacgggc atgcctccct gctgcagcat
     3001 gtgctgttgc tggagcaggc ccggcagcag agcaccctca ttgctgtgcc actccacggg
     3061 cagtccccac tagtgacggg tgaacgtgtg gccaccagca tgcggacggt aggcaagctc
     3121 ccgcggcatc ggcccctgag ccgcactcag tcctcaccgc tgccgcagag tccccaggcc
     3181 ctgcagcagc tggtcatgca acaacagcac cagcagttcc tggagaagca gaagcagcag
     3241 cagctacagc tgggcaagat cctcaccaag acaggggagc tgcccaggca gcccaccacc
     3301 caccctgagg agacagagga ggagctgacg gagcagcagg aggtcttgct gggggaggga
     3361 gccctgacca tgccccggga gggctccaca gagagtgaga gcacacagga agacctggag
     3421 gaggaggacg aggaagacga tggggaggag gaggaggatt gcatccaggt taaggacgag
     3481 gagggcgaga gtggtgctga ggaggggccc gacttggagg agcctggtgc tggatacaaa
     3541 aaactgttct cagatgccca gccgctgcag cctttgcagg tgtaccaggc gcccctcagc
     3601 ctggccactg tgccccacca ggccctgggc cgtacccagt cctcccctgc tgcccctggg
     3661 ggcatgaaga gccccccaga ccagcccgtc aagcacctct tcaccacagg tgtggtctac
     3721 gacacgttca tgctaaagca ccagtgcatg tgcgggaaca cacacgtgca ccctgagcat
     3781 gctggccgga tccagagcat ctggtcccgg ctgcaggaga caggcctgct tagcaagtgc
     3841 gagcggatcc gaggtcgcaa agccacgcta gatgagatcc agacagtgca ctctgaatac
     3901 cacaccctgc tctatgggac cagtcccctc aaccggcaga agctagacag caagaagttg
     3961 ctcggcccca tcagccagaa gatgtatgct gtgctgcctt gtgggggcat cggggtggac
     4021 agtgacaccg tgtggaatga gatgcactcc tccagtgctg tgcgcatggc agtgggctgc
     4081 ctgctggagc tggccttcaa ggtggctgca ggagagctca agaatggatt tgccatcatc
     4141 cggcccccag gacaccacgc cgaggaatcc acagccatgg gattctgctt cttcaactct
     4201 gtagccatca ccgcaaaact cctacagcag aagttgaacg tgggcaaggt cctcatcgtg
     4261 gactgggaca ttcaccatgg caatggcacc cagcaggcgt tctacaatga cccctctgtg
     4321 ctctacatct ctctgcatcg ctatgacaac gggaacttct ttccaggctc tggggctcct
     4381 gaagaggttg gtggaggacc aggcgtgggg tacaatgtga acgtggcatg gacaggaggt
     4441 gtggaccccc ccattggaga cgtggagtac cttacagcct tcaggacagt ggtgatgccc
     4501 attgcccacg agttctcacc tgatgtggtc ctagtctccg ccgggtttga tgctgttgaa
     4561 ggacatctgt ctcctctggg tggctactct gtcaccgcca gatgttttgg ccacttgacc
     4621 aggcagctga tgaccctggc agggggccgg gtggtgctgg ccctggaggg aggccatgac
     4681 ttgaccgcca tctgtgatgc ctctgaggct tgtgtctcgg ctctgctcag tgtagagctg
     4741 cagcccttgg atgaggcagt cttgcagcaa aagcccaaca tcaacgcagt ggccacgcta
     4801 gagaaagtca tcgagatcca gagcaaacac tggagctgtg tgcagaagtt cgccgctggt
     4861 ctgggccggt ccctgcgaga ggcccaagca ggtgagaccg aggaggccga gactgtgagc
     4921 gccatggcct tgctgtcggt gggggccgag caggcccagg ctgcggcagc ccgggaacac
     4981 agccccaggc cggcagagga gcccatggag caggagcctg ccctgggtac ctacccatac
     5041 gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag ctatccttat
     5101 gatgtcccag actacgcata ggatccacta gtccagtgtg gtggaattct gcagatatcc
     5161 agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     5221 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5281 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5341 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5401 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5461 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     5521 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5581 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5641 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5701 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5761 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5821 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5881 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     5941 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6001 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6061 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6121 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6181 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6241 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6301 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     6361 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     6421 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     6481 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     6541 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     6601 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     6661 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     6721 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     6781 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     6841 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     6901 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     6961 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     7021 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     7081 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     7141 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     7201 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7261 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     7321 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7381 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     7441 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7501 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7561 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7621 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7681 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7741 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7801 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7861 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7921 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7981 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8041 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8101 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8161 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8221 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8281 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8341 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8401 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8461 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8521 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8581 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8641 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8701 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8761 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8821 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8881 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8941 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9001 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9061 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9121 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9181 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9241 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9301 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9361 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9421 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9481 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9541 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9601 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggt
//
