LOCUS V029694 6649 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029694 VERSION V029694 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..2169 /label="HABP4(NM_014282)" /note="HABP4(NM_014282)" /gene="HABP4" polyA_signal 2216..2440 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2486..2914 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2928..3257 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3324..4118 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4292..4413 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4462..4478) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4486..4502 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4510..4540) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4555..4576 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4864..5449) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5620..6480) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6481..6585) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgaagggcg ctctggggag tcccgtggct 961 gccgctggcg ccgcgatgca ggagagtttc ggctgcgtgg tggccaaccg cttccatcag 1021 ctgctggacg acgagtcgga cccgttcgac atcctgcgcg aggccgagcg ccggcgccag 1081 cagcagctgc agcgcaagag gcgcgacgag gcggcggcgg cggccggggc cggtccccgc 1141 ggcggcagga gcccagccgg ggcctcgggc cacagagccg gcgcgggcgg ccggagggag 1201 tcgcagaagg agcgcaagag cctcccggcg cccgtcgctc agcggcccga tagccccggg 1261 ggcggcctgc aggcgccggg ccagaagcgg actcctagaa gaggggagca gcaaggatgg 1321 aatgacagcc gtgggccgga ggggatgctc gaaagagctg agcggagatc ctacagggaa 1381 taccgaccct atgagacaga gaggcaggca gacttcacag ctgagaagtt tccagatgaa 1441 aaaccaggtg ataggtttga tcgagacaga ccgttgagag gacgtggagg cccgagaggg 1501 ggtatgcgcg gcagaggcag aggtggccct gggaacagag tttttgacgc ttttgaccag 1561 agaggaaagc gagaatttga aagatatggt gggaatgaca aaatagcagt cagaactgaa 1621 gacaacatgg gtggatgtgg agttcgaacc tggggatcgg gtaaagatac cagtgatgtg 1681 gagccaactg caccgatgga ggaacccaca gtggtggagg agtcccaggg caccccggaa 1741 gaggagtctc cagccaaagt tcctgagttg gaggtagaag aagaaaccca agttcaagag 1801 atgactttag atgagtggaa aaatcttcaa gaacagacca gaccaaagcc tgagtttaac 1861 atccggaaac cagaatccac tgttccttcc aaagccgtgg tgattcacaa gtcaaaatac 1921 agagatgata tggtaaaaga tgactatgag gacgattccc atgttttccg gaaacccgcc 1981 aatgacatca catcccagct ggagattaat tttggtaacc tccctcgtcc tgggcgtgga 2041 gccagaggag gcacccgggg aggccgggga aggatcagga gggcagagaa ctatggaccc 2101 agagcagaag tggtgatgca agatgttgcc cccaacccag atgacccgga agatttccct 2161 gcgctgtctt gactcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg 2221 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 2281 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 2341 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 2401 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 2461 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 2521 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 2581 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 2641 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 2701 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 2761 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 2821 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 2881 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 2941 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 3001 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3061 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 3121 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 3181 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 3241 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 3301 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 3361 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 3421 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 3481 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 3541 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 3601 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 3661 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 3721 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 3781 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 3841 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 3901 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 3961 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 4021 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 4081 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 4141 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 4201 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 4261 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 4321 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 4381 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 4441 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 4501 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 4561 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 4621 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4681 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4741 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 4801 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 4861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 4921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 4981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 5041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 5101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 5161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 5221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 5281 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 5341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 5401 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 5461 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 5521 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 5581 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 5641 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 5701 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 5761 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 5821 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 5881 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 5941 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 6001 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 6061 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 6121 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 6181 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 6241 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 6301 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 6361 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 6421 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 6481 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 6541 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 6601 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt //