LOCUS       V031362                 8138 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031362
VERSION     V031362
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3625
                     /label="GRIA1(NM_000827)"
                     /note="GRIA1(NM_000827)"
                     /gene="GRIA1"
     polyA_signal    3705..3929
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3975..4403
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4417..4746
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4813..5607
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5781..5902
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5951..5967)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5975..5991
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5999..6029)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6044..6065
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6353..6938)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7109..7969)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7970..8074)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg cagcacattt ttgccttctt ctgcaccggt ttcctaggcg cggtagtagg
      961 tgccaatttc cccaacaata tccagatcgg gggattattt ccaaaccagc agtcacagga
     1021 acatgctgct tttagatttg ctttgtcgca actcacagag cccccgaagc tgctccccca
     1081 gattgatatt gtgaacatca gcgacagctt tgagatgacc tatagattct gttcccagtt
     1141 ctccaaagga gtctatgcca tctttgggtt ttatgaacgt aggactgtca acatgctgac
     1201 ctccttttgt ggggccctcc acgtctgctt cattacgccg agctttcccg ttgatacatc
     1261 caatcagttt gtccttcagc tgcgccctga actgcaggat gccctcatca gcatcattga
     1321 ccattacaag tggcagaaat ttgtctacat ttatgatgcc gaccggggct tatccgtcct
     1381 gcagaaagtc ctggatacag ctgctgagaa gaactggcag gtgacagcag tcaacatttt
     1441 gacaaccaca gaggagggat accggatgct ctttcaggac ctggagaaga aaaaggagcg
     1501 gctggtggtg gtggactgtg aatcagaacg cctcaatgct atcttgggcc agattataaa
     1561 gctagagaag aatggcatcg gctaccacta cattcttgca aatctgggct tcatggacat
     1621 tgacttaaac aaattcaagg agagtggcgc caatgtgaca ggtttccagc tggtgaacta
     1681 cacagacact attccggcca agatcatgca gcagtggaag aatagtgatg ctcgagacca
     1741 cacacgggtg gactggaaga gacccaagta cacctctgcg ctcacctacg atggggtgaa
     1801 ggtgatggct gaggctttcc agagcctgcg gaggcagaga attgatatat ctcgccgggg
     1861 gaatgctggg gattgtctgg ctaacccagc tgttccctgg ggccaaggga tcgacatcca
     1921 gagagctctg cagcaggtgc gatttgaagg tttaacagga aacgtgcagt ttaatgagaa
     1981 aggacgccgg accaactaca cgctccacgt gattgaaatg aaacatgacg gcatccgaaa
     2041 gattggttac tggaatgaag atgataagtt tgtccctgca gccaccgatg cccaagctgg
     2101 gggcgataat tcaagtgttc agaacagaac atacatcgtc acaacaatcc tagaagatcc
     2161 ttatgtgatg ctcaagaaga acgccaatca gtttgagggc aatgaccgtt acgagggcta
     2221 ctgtgtagag ctggcggcag agattgccaa gcacgtgggc tactcctacc gtctggagat
     2281 tgtcagtgat ggaaaatacg gagcccgaga ccctgacacg aaggcctgga atggcatggt
     2341 gggagagctg gtctatggaa gagcagatgt ggctgtggct cccttaacta tcactttggt
     2401 ccgggaagaa gttatagatt tctccaaacc atttatgagt ttggggatct ccatcatgat
     2461 taaaaaacca cagaaatcca agccgggtgt cttctccttc cttgatcctt tggcttatga
     2521 gatttggatg tgcattgttt ttgcctacat tggagtgagt gttgtcctct tcctggtcag
     2581 ccgcttcagt ccctatgaat ggcacagtga agagtttgag gaaggacggg accagacaac
     2641 cagtgaccag tccaatgagt ttgggatatt caacagtttg tggttctccc tgggagcctt
     2701 catgcagcaa ggatgtgaca tttctcccag gtccctgtct ggtcgcatcg ttggtggcgt
     2761 ctggtggttc ttcaccttaa tcatcatctc ctcatataca gccaatctgg ccgccttcct
     2821 gaccgtggag aggatggtgt ctcccattga gagtgcagag gacctagcga agcagacaga
     2881 aattgcctac gggacgctgg aagcaggatc tactaaggag ttcttcagga ggtctaaaat
     2941 tgctgtgttt gagaagatgt ggacatacat gaagtcagca gagccatcag tttttgtgcg
     3001 gaccacagag gaggggatga ttcgagtgag gaaatccaaa ggcaaatatg cctacctcct
     3061 ggagtccacc atgaatgagt acattgagca gcggaaaccc tgtgacacca tgaaggtggg
     3121 aggtaacttg gattccaaag gctatggcat tgcaacaccc aaggggtctg ccctgagaaa
     3181 tccagtaaac ctggcagtgt taaaactgaa cgagcagggg cttttggaca aattgaaaaa
     3241 caaatggtgg tacgacaagg gcgagtgcgg cagcggggga ggtgattcca aggacaagac
     3301 aagcgctctg agcctcagca atgtggcagg cgtgttctac atcctgatcg gaggacttgg
     3361 actagccatg ctggttgcct taatcgagtt ctgctacaaa tcccgtagtg aatccaagcg
     3421 gatgaagggt ttttgtttga tcccacagca atccatcaac gaagccatac ggacatcgac
     3481 cctcccccgc aacagcgggg caggagccag cagcggcggc agtggagaga atggtcgggt
     3541 ggtcagccat gacttcccca agtccatgca atcgattcct tgcatgagcc acagttcagg
     3601 gatgcccttg ggagccacgg gattgtaaga attctgcaga tatccagcac agtggcggcc
     3661 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     3721 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3781 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3841 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3901 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     3961 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4021 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4081 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4141 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4201 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4261 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4321 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4381 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4441 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4501 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4561 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4621 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4681 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4741 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     4801 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4861 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4921 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4981 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5041 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5101 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5161 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     5221 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     5281 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5341 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5401 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5461 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5521 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5581 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     5641 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     5701 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     5761 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     5821 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5881 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     5941 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6001 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6061 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6121 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6181 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6241 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6301 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6361 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6421 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6481 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6541 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6601 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6661 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6721 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6781 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6841 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt
     6901 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6961 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7021 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7081 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7141 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7201 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7261 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7321 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7381 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7441 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7501 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7561 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7621 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7681 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7741 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7801 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7861 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7921 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7981 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8041 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8101 acgtcgacgg atcgggagat ctcccgatcc cctatggt
//
