LOCUS       pcDNA3.1-Esr2(mouse)    7088 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027760
VERSION     V027760
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..2608
                     /label="Esr2(NM_207707)"
                     /note="Esr2(NM_207707)"
                     /gene="Esr2"
     polyA_signal    2655..2879
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      2925..3353
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3367..3696
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3763..4557
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4731..4852
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(4901..4917)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4925..4941
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4949..4979)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4994..5015
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5303..5888)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6059..6919)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6920..7024)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg tccatctgtg cctcttctca caaggatttt tctcagctga gacctacgca
      961 agacatggag atcaaaaact caccatcaag ccttacttcc cctgcttctt ataactgtag
     1021 ccagtccatc ctacccttgg agcatggtcc catctatatc ccttcctcct atgtagagag
     1081 ccgtcacgaa tactcagcca tgacattcta cagtcctgct gtgatgaact acagtgttcc
     1141 cagcagcacc ggtaacctgg aaggtgggcc tgttcgccag actgcaagcc caaatgtgct
     1201 atggccaact tctggacacc tctctccttt agccacccac tgccaatcat cgcttctcta
     1261 tgcagaacct caaaagagtc cttggtgtga agcaagatca ctagaacaca ccttgcctgt
     1321 aaacagagag accctgaaga ggaagcttgg cgggagcggt tgtgccagcc ctgttactag
     1381 tccaagcgcc aagagggatg ctcacttctg cgccgtctgc agtgattatg catctgggta
     1441 tcattacggt gtctggtcct gtgaaggatg taaggccttt tttaaaagaa gcattcaagg
     1501 acataatgac tatatctgtc cagccacgaa tcagtgtacc atagacaaga accggcgtaa
     1561 aagctgccag gcctgccgac ttcgcaagtg ttacgaagta ggaatggtca agtgtggatc
     1621 caggagagaa aggtgtgggt accgaatagt acgaagacag agaagtgcca gcgagcaggt
     1681 gcattgcctg aacaaagcca agagaaccag tgggcacaca ccccgggtga aggagctact
     1741 gctgaactct ctgagtcccg agcagctggt gctcaccctg ctggaagctg agccacccaa
     1801 tgtgctagtg agccgtccca gcatgccctt caccgaggcc tccatgatga tgtccctcac
     1861 gaagctggct gacaaggaac tggtgcacat gattggctgg gccaagaaaa tccctggctt
     1921 tgtggagctc agcctgttgg accaagtccg cctcttggaa agctgctgga tggaggtgct
     1981 gatggtgggg ctgatgtggc gctccatcga ccaccccggc aagctcatct ttgctccaga
     2041 cctcgttctg gacaggtcct cagaagaccc tcactggcac gttgcgcaga cgaagagtgc
     2101 tgtcccaagg gatgagggga agtgcgtgga agggattctg gaaatctttg acatgctcct
     2161 ggcgacgacg gcacggttcc gtgagttaaa actgcagcac aaagaatatc tgtgtgtgaa
     2221 ggccatgatt ctcctcaact ccagtatgta ccccttggct accgcaagcc aggaagcaga
     2281 gagtagccgg aagctgacac acctattgaa cgcagtgaca gatgccctgg tctgggtgat
     2341 ttcgaagagt ggaatctctt cccagcagca gtcagtccgt ctggccaacc tcctgatgct
     2401 tctttctcat gtcaggcaca tcagtaacaa gggcatggaa catctgctca gcatgaagtg
     2461 caaaaatgtg gtcccggtgt acgacctgct gctggagatg ctgaatgctc acacgcttcg
     2521 agggtacaag tcctcaatct cggggtctga gtgctgctcg acagaggaca gtaagagcaa
     2581 agagggctcc cagaacctcc agtcacagtg actcgagtct agagggcccg tttaaacccg
     2641 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     2701 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     2761 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2821 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     2881 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     2941 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3001 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3061 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3121 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3181 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3241 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3301 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     3361 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3421 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3481 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3541 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3601 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     3661 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     3721 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     3781 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     3841 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     3901 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     3961 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     4021 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     4081 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     4141 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     4201 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     4261 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     4321 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     4381 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     4441 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     4501 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     4561 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     4621 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     4681 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     4741 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4801 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4861 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4921 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4981 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5041 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5701 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5821 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5881 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5941 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6001 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6061 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6121 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6181 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6241 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6301 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6361 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6421 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6481 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6541 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6601 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6661 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6721 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6781 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6841 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6901 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6961 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7021 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     7081 cctatggt
//
