LOCUS V035955 6488 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035955 VERSION V035955 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..2008 /label="ELAVL3(NM_001420)" /note="ELAVL3(NM_001420)" /gene="ELAVL3" polyA_signal 2055..2279 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2325..2753 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2767..3096 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3163..3957 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4131..4252 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4301..4317) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4325..4341 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4349..4379) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4394..4415 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4703..5288) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5459..6319) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6320..6424) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gtcactcaga tactgggggc catggagtct caggtggggg ggggcccggc 961 cggcccggcc ctgcccaacg ggccactcct tggtacaaat ggagccactg acgacagcaa 1021 gaccaacctc atcgtcaact acctgcccca gaacatgacc caggatgagt tcaagagtct 1081 cttcggcagc attggcgaca tcgagtcctg caagttggtt cgggacaaga tcacagggca 1141 gagccttggc tacgggtttg tgaactattc tgaccccaat gatgcagaca aagccatcaa 1201 caccctcaac ggcctcaaat tacagacgaa gaccatcaag gtgtcctatg ccagacccag 1261 ttcagcatcc atccgggatg ctaacctgta cgtcagcggg ctccccaaga ccatgagcca 1321 gaaagagatg gagcagctct tctcccagta cggccgcatc atcacgtccc gcatcctggt 1381 ggaccaggtc acaggtgtct ctcggggtgt gggattcatc cgctttgaca agaggattga 1441 ggccgaagag gctatcaaag gactgaatgg gcagaagccg ctgggcgcag ctgagcccat 1501 cacagtcaag ttcgcgaaca acccaagtca gaagacgggg caggcgctgc tcacccacct 1561 ctaccagtca tccgcccggc gctacgcagg ccccctacac catcagaccc agcgtttccg 1621 gctggacaat ttgctcaaca tggcctacgg cgtcaagagt cccctgtcgc tcatcgccag 1681 gttctcgccg atcgccatcg atggtatgag cggcctggcg ggcgtgggcc tgtcgggggg 1741 cgcggcgggc gccggctggt gcatcttcgt gtacaacctg tcaccggagg cagacgagag 1801 cgtgctgtgg cagctgttcg ggccttttgg ggcagtcacc aacgtcaagg tcatccgtga 1861 tttcaccacc aacaagtgca agggtttcgg cttcgtgacc atgaccaact atgacgaggc 1921 ggccatggcc atcgccagcc tgaacggcta tcgcctgggc gagcgcgtgc tgcaggtctc 1981 cttcaagacc agcaaacagc acaaggcgtg actcgagtct agagggcccg tttaaacccg 2041 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 2101 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 2161 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 2221 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 2281 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 2341 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 2401 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 2461 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 2521 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 2581 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 2641 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 2701 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 2761 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 2821 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 2881 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 2941 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 3001 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 3061 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 3121 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 3181 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 3241 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 3301 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 3361 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 3421 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 3481 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 3541 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 3601 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 3661 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 3721 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 3781 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 3841 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 3901 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 3961 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 4021 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 4081 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 4141 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 4201 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 4261 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 4321 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 4381 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 4441 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 4501 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4561 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4621 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4681 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4741 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4801 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4861 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4921 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4981 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5041 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5101 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 5161 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5221 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 5281 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 5341 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 5401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 5461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 5521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 5581 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 5641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 5701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 5761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 5821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 5881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 5941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 6001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 6061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 6121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 6181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 6241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 6301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 6361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 6421 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 6481 cctatggt //