LOCUS       V036666                 8203 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036666
VERSION     V036666
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             964..3693
                     /label="Ddr1(NM_013137)"
                     /note="Ddr1(NM_013137)"
                     /gene="Ddr1"
     CDS             3700..3765
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3803..4027
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4073..4501
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4515..4844
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4911..5705
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5879..6000
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6049..6065)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6073..6089
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6097..6127)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6142..6163
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6451..7036)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7207..8067)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8068..8172)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc
      961 accatgggga cagggaccct ctcatctctc ctgctgctgc tactcttggt gacaattgga
     1021 gatgctgaca tgaagggaca ttttgaccca gccaagtgcc gctatgccct gggcatgcag
     1081 gaccgcacca ttccagacag cgatatctct gtgtccagct cctggtcgga ctctaccgcc
     1141 gcccgccaca gcaggctgga aagcagtgat ggagatgggg cttggtgccc tgcagggcct
     1201 gtgttcccca aagaagagga gtacctgcag gtggaccttc gtaggctaca cctggtggct
     1261 ctggtgggca cccagggccg ccatgctggg ggtctgggca aagagttctc ccgaagctac
     1321 cggttgcgtt actcccgaga tggccgacga tggatggact ggaaagacag atggggtcag
     1381 gaggtgattt cgggtaacga ggatcctggg ggagtggtgc tgaaggatct cgggcccccc
     1441 atggtggccc ggctggtccg tttctacccc agggctgacc gggtcatgag tgtctgtctt
     1501 cgggtggagc tctatggctg cctctggcgg gacggactct tgtcctatac agcccccgtg
     1561 gggcagacca tgcaattatc tgagatggtg tatctcaatg attccactta cgatggatat
     1621 actgctggag ggctgcagta cggtggtctg ggccaactgg cagacggcgt ggtggggctg
     1681 gatgatttca ggcagagcca ggagctgcgg gtttggccag gctatgacta tgtgggatgg
     1741 agcaaccata gcttccccag cggctacgtg gagatggagt ttgagtttga ccggctgagg
     1801 tctttccaga ccatgcaggt ccactgtaac aacatgcaca ctctgggagc ccgcctccca
     1861 ggcggggtgg aatgccggtt taaaaggggt ccggccatgg cctgggaagg agagcctgta
     1921 cgccatgccc tggggggtag ccttggagac cccagagcca gggccatctc ggtgcccctg
     1981 ggtggtcacg tgggccgctt cctgcagtgc agattcctct ttgcaggacc ctggttactc
     2041 ttcagtgaga tctctttcat ctcggatgtg gtgaacgact cgtctgacac cttcccacca
     2101 gccccctggt ggccgcctgg cccgcctccc accaacttca gcagcttgga gctggagccc
     2161 cggggtcaac agcctgtggc caaggcggag gggagcccaa ctgccatcct cattggctgc
     2221 ctggtggcca tcatccttct cctcctcctt atcattgccc tgatgctctg gaggctgcac
     2281 tggcgccggc tgctcagcaa ggcagagcgc cgggtgttgg aggaggagct gacggttcac
     2341 ctctctgtcc ctggggacac catcctcatc aacaaccgcc cagggccccg agagccaccc
     2401 ccttaccagg aaccccggcc tcgggggact ccaactcact ctgcgccctg cgtccccaat
     2461 ggctctgcgt tgctgctctc caatccggcc taccgcctcc ttctggccac ttacgcccgt
     2521 ccccctcgag gcccgggccc ccccacaccc gcctgggcca aacccaccaa cacccaggcc
     2581 tgcagtgggg actacatgga gcccgagaag ccgggcgccc cacttctacc cccacctccc
     2641 cagaacagcg tcccccatta cgccgaggct gacattgtca ccctgcaggg cgtcacaggg
     2701 ggcaacacct acgctgttcc cgcactgccc ccaggggcgg ttggggatgg gccccccaga
     2761 gtggatttcc ctcggtcacg gctccgtttc aaggagaagc tcggcgaggg ccaatttggg
     2821 gaggtacatc tctgtgaagt agaggaccct caagatctgg tcactagtga cttccctatc
     2881 agtgtgcaaa agggacaccc cttgctggtg gcagtgaaga tcctccggcc agatgccacc
     2941 aaaaatgcca ggaatgactt cctgaaggag gtaaagatca tgtcacggct gaaggaccca
     3001 aacatcatcc ggctcctggg tgtgtgtgtg caggatgacc ctctctgcat gattacagac
     3061 tacatggaga acggtgacct gaaccaattc ctcagtgccc accagctgga gaacaaggtc
     3121 actcaggggc ttcctgggga cagagagtct gaccaggggc ccacaatcag ctaccctatg
     3181 ctgttacacg tgggggccca gatcgcctct ggcatgcgat atctggccac ccttaacttt
     3241 gtgcatcggg acctggccac ccggaactgc ttggttgggg aaaatttcac catcaaaatc
     3301 gccgactttg gcatgagccg gaatctctac gctggggatt attaccgtgt acagggccgg
     3361 gcagtgctgc ccatcaggtg gatggcctgg gagtgtatcc tcatggggaa gttcacaaca
     3421 gccagtgatg tgtgggcctt cggagtgacc ctgtgggagg tgcttatgct ctgcaggtca
     3481 cagcccttcg ggcaactcac agacgagcag gtcatcgaga acgccggcga gttcttcagg
     3541 gaccagggcc ggcaggtata cttatccagg ccacccgcct gcccgcagac cttgtatgag
     3601 ctgatgctcc ggtgttggag ccgggagccg gagcagcggc cgcccttctc ccagcttcat
     3661 cggttcctgg cggatgatgc actcaacaca gtgctcgagg actacaaaga ccatgacggt
     3721 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagtg agggcccgtt
     3781 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3841 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3901 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3961 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4021 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     4081 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4141 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4201 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4261 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4321 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4381 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4441 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4501 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4561 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4621 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4681 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4741 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4801 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     4861 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4921 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4981 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5041 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5101 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5161 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5221 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5281 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5341 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5401 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5461 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5521 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5581 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5641 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5701 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5761 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5821 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6001 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     6061 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6121 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6181 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6241 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6961 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     7021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8161 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
