LOCUS       V046604                 7039 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046604
VERSION     V046604
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..2478
                     /label="DHCR24(NM_014762)"
                     /note="DHCR24(NM_014762)"
                     /gene="DHCR24"
     CDS             2485..2511
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2515..2541
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2545..2571
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    2606..2830
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      2876..3304
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3318..3647
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3714..4508
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4682..4803
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(4852..4868)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4876..4892
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4900..4930)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4945..4966
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5254..5839)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6010..6870)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6871..6975)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggagcccg ccgtgtcgct ggccgtgtgc
      961 gcgctgctct tcctgctgtg ggtgcgcctg aaggggctgg agttcgtgct catccaccag
     1021 cgctgggtgt tcgtgtgcct cttcctcctg ccgctctcgc ttatcttcga tatctactac
     1081 tacgtgcgcg cctgggtggt gttcaagctc agcagcgctc cgcgcctgca cgagcagcgc
     1141 gtgcgggaca tccagaagca ggtgcgggaa tggaaggagc agggtagcaa gaccttcatg
     1201 tgcacggggc gccctggctg gctcactgtc tcactacgtg tcgggaagta caagaagaca
     1261 cacaaaaaca tcatgatcaa cctgatggac attctggaag tggacaccaa gaaacagatt
     1321 gtccgtgtgg agcccttggt gaccatgggc caggtgactg ccctgctgac ctccattggc
     1381 tggactctcc ccgtgttgcc tgagcttgat gacctcacag tggggggctt gatcatgggc
     1441 acaggcatcg agtcatcatc ccacaagtac ggcctgttcc aacacatctg cactgcttac
     1501 gagctggtcc tggctgatgg cagctttgtg cgatgcactc cgtccgaaaa ctcagacctg
     1561 ttctatgccg taccctggtc ctgtgggacg ctgggtttcc tggtggccgc tgagatccgc
     1621 atcatccctg ccaagaagta cgtcaagctg cgtttcgagc cagtgcgggg cctggaggct
     1681 atctgtgcca agttcaccca cgagtcccag cggcaggaga accacttcgt ggaagggctg
     1741 ctctactccc tggatgaggc tgtcattatg acaggggtca tgacagatga ggcagagccc
     1801 agcaagctga atagcattgg caattactac aagccgtggt tctttaagca tgtggagaac
     1861 tatctgaaga caaaccgaga gggcctggag tacattccct tgagacacta ctaccaccgc
     1921 cacacgcgca gcatcttctg ggagctccag gacattatcc cctttggcaa caaccccatc
     1981 ttccgctacc tctttggctg gatggtgcct cccaagatct ccctcctgaa gctgacccag
     2041 ggtgagaccc tgcgcaagct gtacgagcag caccacgtgg tgcaggacat gctggtgccc
     2101 atgaagtgcc tgcagcaggc cctgcacacc ttccaaaacg acatccacgt ctaccccatc
     2161 tggctgtgtc cgttcatcct gcccagccag ccaggcctag tgcaccccaa aggaaatgag
     2221 gcagagctct acatcgacat tggagcatat ggggagccgc gtgtgaaaca ctttgaagcc
     2281 aggtcctgca tgaggcagct ggagaagttt gtccgcagcg tgcatggctt ccagatgctg
     2341 tatgccgact gctacatgaa ccgggaggag ttctgggaga tgtttgatgg ctccttgtac
     2401 cacaagctgc gagagaagct gggttgccag gacgccttcc ccgaggtgta cgacaagatc
     2461 tgcaaggccg ccaggcacct cgagtaccca tacgacgtac ctgattatgc aacttatcct
     2521 tatgatgtac ctgattatgc tagctatcct tatgatgtcc cagactacgc atgagggccc
     2581 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     2641 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     2701 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     2761 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     2821 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     2881 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2941 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3001 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3061 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3181 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3241 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3301 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3361 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3421 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3481 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3541 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     3601 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     3661 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     3721 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     3781 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     3841 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     3901 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     3961 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4021 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4081 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4141 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4201 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     4261 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4321 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4381 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4441 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4501 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     4561 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     4621 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     4681 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4741 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4801 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     4861 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     4921 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4981 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5041 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5101 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5161 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5221 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5281 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5341 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5401 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5461 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5521 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5581 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5641 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5701 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5761 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg
     5821 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5881 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5941 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6001 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6061 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6121 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6181 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6241 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6301 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6361 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6421 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6481 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6541 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6601 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6661 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6721 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6781 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6841 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6901 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6961 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     7021 tctcccgatc ccctatggt
//
