LOCUS V020481 8846 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020481 VERSION V020481 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..4336 /label="DAB2IP(NM_032552)" /note="DAB2IP(NM_032552)" /gene="DAB2IP" CDS 4343..4408 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 4446..4670 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4716..5144 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5158..5487 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5554..6348 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6522..6643 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6692..6708) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6716..6732 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6740..6770) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6785..6806 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7094..7679) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7850..8710) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8711..8815) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggagcccg actcccttct 961 ggaccaagac gactcctacg agtcgcctca agaaaggccg ggctctcggc gcagcctgcc 1021 tggcagcctt tccgagaaga gccccagcat ggagccctcg gccgccacgc cgttccgggt 1081 cacgggcttc ctcagccgcc gcctcaaggg ctccatcaag cgcaccaaga gccagcccaa 1141 gctggaccgc aaccacagct tccgccacat cctgccgggg ttccggagcg ccgccgccgc 1201 cgccgcggac aatgagaggt cccatctgat gccgaggctg aaggagtctc gctcccacga 1261 gtccctgctc agccccagca gtgcggtgga ggcgctggac ctcagcatgg aggaagaggt 1321 ggtcatcaag cccgtgcaca gcagcatcct tggccaggac tactgcttcg aggtgacgac 1381 gtcatcagga agcaagtgct tttcctgccg gtctgcagct gagcgggata agtggatgga 1441 gaacctccgg cgagcggtgc atcccaacaa ggacaacagc cggcgtgtgg agcacatcct 1501 gaagctgtgg gtgatcgagg ccaaggacct gccagccaag aagaagtacc tgtgcgagct 1561 gtgcctggac gatgtgctct atgcccgcac cacgggcaag ctcaagacgg acaatgtttt 1621 ctggggcgag cacttcgagt tccacaactt gccgcctctg cgcacggtca ctgtccacct 1681 gtaccgggag accgacaaga agaagaagaa ggagcgcaac agttacctgg gcctggtgag 1741 cctacctgct gcctcggtgg ccgggcggca gttcgtggag aagtggtacc cggtggtgac 1801 gcccaacccc aagggcggca agggccctgg acccatgatc cgcatcaagg cgcgctacca 1861 aaccatcacc atcctgccca tggagatgta caaagagttc gctgagcaca tcaccaacca 1921 ctacctgggg ctgtgtgcag ccctcgagcc catcctcagt gccaagacca aggaggagat 1981 ggcatctgcc ctggtgcaca tcctgcagag cacgggcaag gtgaaggact tcctgacaga 2041 cctgatgatg tcagaggtgg accgctgcgg ggacaacgag cacctcatct tccgggagaa 2101 cacactggcc accaaggcca ttgaggagta cctcaagcta gtgggccaga agtacctgca 2161 ggacgcccta ggtgagttca tcaaagcgct gtatgagtca gatgagaact gcgaagtgga 2221 tcccagcaag tgctcggccg ctgacctccc agagcaccag ggcaacctca agatgtgctg 2281 cgagctggcc ttctgcaaga tcatcaactc ctactgtgtc ttcccacggg agttgaaaga 2341 ggtgtttgcc tcgtggaggc aggagtgcag cagtcgcggc cgcccggaca tcagtgagcg 2401 gctcatcagc gcctccctct tcctgcgctt cctctgccca gccatcatgt cgccctcact 2461 cttcaacctg ctgcaggagt accctgatga ccgcactgcc cgcaccctca ccctcatcgc 2521 caaggtcacc cagaacctgg ccaactttgc caaatttggc agcaaggagg aatacatgtc 2581 cttcatgaac cagttcctag agcatgagtg gaccaacatg cagcgcttcc tgctggagat 2641 ctccaacccc gagaccctct ccaatacagc cggcttcgag ggctacatcg acctgggccg 2701 cgagctctcc agcctgcact cactgctctg ggaggccgtc agccagctgg agcagagcat 2761 agtatccaaa ctgggacccc tgcctcggat cctgagggac gtccacacag cactgagcac 2821 cccaggtagc gggcagctcc cagggaccaa tgacctggcc tccacaccgg gctctggcag 2881 cagcagcatc tcagctgggc tgcagaagat ggtgattgag aacgatcttt ccggtctgat 2941 agatttcacc cggttaccgt ctccaacccc cgaaaacaag gacttgtttt ttgtcacaag 3001 gtcctccggg gtccagccct cacctgcccg cagctcgagt tactcggaag ccaacgagcc 3061 tgatcttcag atggccaacg gtggcaagag cctctccatg gtggacctcc aggacgcccg 3121 cacgctggat ggggaggcag gctccccggc gggccccgac gtcctcccca cagatgggca 3181 ggccgctgca gctcagctgg tggccgggtg gccggcccgg gcaaccccag tgaacctggc 3241 agggctggcc acggtgcggc gggcaggcca gacaccaacc acaccaggca cctccgaggg 3301 cgcgccaggc cggccccagc tgttggcacc gctctccttc cagaaccctg tgtaccagat 3361 ggcggctggc ctgccgctgt caccccgtgg ccttggcgac tcaggctctg agggccacag 3421 ctccctgagc tcacacagca acagcgagga gttggcggct gctgccaagc tgggaagttt 3481 cagcactgcc gcggaggagc tggctcggcg gcccggtgag ctggcacggc gacagatgtc 3541 actgactgaa aaaggcgggc agcccacggt gccacggcag aacagtgctg gcccccagag 3601 gaggatcgac cagcctccgc ccccaccccc gccgccacct cctgcccccc gcggccggac 3661 gccccccaac ctgctgagca ccctgcagta cccaagaccc tcaagcggaa ccctggcgtc 3721 ggcctcacct gattgggtgg gccccagtac ccgcctgagg cagcagtcct cttcctccaa 3781 gggggacagc ccagaactga agccacgggc agtgcacaag cagggccctt cacctgtgag 3841 ccccaatgcc ctggaccgca cagccgcttg gctcttgacc atgaacgcgc agttgttaga 3901 agacgagggc ctgggcccag acccccccca cagggatagg ctaaggagta aggacgagct 3961 cagccaagca gaaaaggacc tggcggtgct gcaggacaag ctgcgaatct ccaccaagaa 4021 gctggaggag tatgagaccc tgttcaagtg ccaggaggag acgacgcaga agctggtgct 4081 ggagtaccag gcacggctgg aggagggcga ggagcggctg cggcggcagc aggaggacaa 4141 ggacatccag atgaagggca tcatcagcag gttgatgtcc gtggaggaag aactgaagaa 4201 ggaccacgca gagatgcaag cggctgtgga ctccaaacag aagatcattg atgcccagga 4261 gaagcgcatt gcctcgttgg atgccgccaa tgcccgcctc atgagtgccc tgacccagct 4321 gaaagagagt atgcatctcg aggactacaa agaccatgac ggtgattata aagatcatga 4381 catcgactac aaggatgacg atgacaagta gtgagggccc gtttaaaccc gctgatcagc 4441 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 4501 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 4561 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 4621 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 4681 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 4741 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 4801 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 4861 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 4921 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 4981 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 5041 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 5101 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg 5161 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 5221 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 5281 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 5341 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 5401 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc 5461 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga 5521 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 5581 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 5641 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 5701 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg 5761 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 5821 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 5881 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 5941 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 6001 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 6061 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc 6121 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 6181 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 6241 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 6301 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 6361 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 6421 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 6481 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt 6541 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 6601 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt 6661 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 6721 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 6781 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 6841 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 6901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 6961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 7021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 7081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 7141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 7201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 7261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 7321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 7381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 7441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 7501 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 7561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 7621 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7681 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7741 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7801 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7861 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7921 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7981 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 8041 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 8101 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 8161 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 8221 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 8281 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 8341 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 8401 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8461 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8521 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8581 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8641 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8701 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8761 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8821 ttccccgaaa agtgccacct gacgtc //