LOCUS       V036798                 7787 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036798
VERSION     V036798
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3226
                     /label="CPT1A(NM_001876)"
                     /note="CPT1A(NM_001876)"
                     /gene="CPT1A"
     CDS             3233..3259
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3263..3289
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3293..3319
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3354..3578
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3624..4052
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4066..4395
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4462..5256
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5430..5551
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5600..5616)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5624..5640
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5648..5678)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5693..5714
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6002..6587)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6758..7618)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7619..7723)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcagaagctc accaagctgt ggcctttcag ttcacggtca ctccggacgg
      961 gattgacctg cggctgagcc atgaagctct tagacaaatc tatctctctg gacttcattc
     1021 ctggaaaaag aagttcatca gattcaagaa cggcatcatc actggcgtgt acccggcaag
     1081 cccctccagt tggcttatcg tggtggtggg cgtgatgaca acgatgtacg ccaagatcga
     1141 cccctcgtta ggaataattg caaaaatcaa tcggactctg gaaacggcca actgcatgtc
     1201 cagccagacg aagaacgtgg tcagcggcgt gctgtttggc accggcctgt gggtggccct
     1261 catcgtcacc atgcgctact ccctgaaagt gctgctctcc taccacgggt ggatgttcac
     1321 tgagcacggc aagatgagtc gtgccaccaa gatctggatg ggtatggtca agatcttttc
     1381 aggccgaaaa cccatgttgt acagcttcca gacatcgctg cctcgcctgc cggtcccggc
     1441 tgtcaaagac actgtgaaca ggtatctaca gtcggtgagg cctcttatga aggaagaaga
     1501 cttcaaacgg atgacagcac ttgctcaaga ttttgctgtc ggtcttggac caagattaca
     1561 gtggtatttg aagttaaaat cctggtgggc tacaaattac gtgagcgact ggtgggagga
     1621 gtacatctac ctccgaggac gagggccgct catggtgaac agcaactatt atgccatgga
     1681 tctgctgtat atccttccaa ctcacattca ggcagcaaga gccggcaacg ccatccatgc
     1741 catcctgctt tacaggcgca aactggaccg ggaggaaatc aaaccaattc gtcttttggg
     1801 atccacgatt ccactctgct ccgctcagtg ggagcggatg tttaatactt cccggatccc
     1861 aggagaggag acagacacca tccagcacat gagagacagc aagcacatcg tcgtgtacca
     1921 tcgaggacgc tacttcaagg tctggctcta ccatgatggg cggctgctga agccccggga
     1981 gatggagcag cagatgcaga ggatcctgga caatacctcg gagcctcagc ccggggaggc
     2041 caggctggca gccctcaccg caggagacag agttccctgg gccaggtgtc gtcaggccta
     2101 ttttggacgt gggaaaaata agcagtctct tgatgctgtg gagaaagcag cgttctttgt
     2161 gacgttagat gaaactgaag aaggatacag aagtgaagac ccggatacgt caatggacag
     2221 ctacgccaaa tctctactac acggccgatg ttacgacagg tggtttgaca agtcgttcac
     2281 gtttgttgtc ttcaaaaacg ggaagatggg cctcaacgct gaacactcct gggcagatgc
     2341 gccgatcgtg gcccaccttt gggagtacgt catgtccatt gacagcctcc agctgggcta
     2401 tgcggaggat gggcactgca aaggcgacat caatccgaac attccgtacc ccaccaggct
     2461 gcagtgggac atcccggggg aatgtcaaga ggttatagag acctccctga acaccgcaaa
     2521 tcttctggca aacgacgtgg atttccattc cttcccattc gtagcctttg gtaaaggaat
     2581 catcaagaaa tgtcgcacga gcccagacgc ctttgtgcag ctggccctcc agctggcgca
     2641 ctacaaggac atgggcaagt tttgcctcac atacgaggcc tccatgaccc ggctcttccg
     2701 agaggggagg acggagaccg tgcgctcctg caccactgag tcatgcgact tcgtgcgggc
     2761 catggtggac ccggcccaga cggtggaaca gaggctgaag ttgttcaagt tggcgtctga
     2821 gaagcatcag catatgtatc gcctcgccat gaccggctct gggatcgatc gtcacctctt
     2881 ctgcctttac gtggtgtcta aatatctcgc tgtggagtcc cctttcctta aggaagtttt
     2941 atctgagcct tggagattat caacaagcca gacccctcag cagcaagtgg agctgtttga
     3001 cttggagaat aacccagagt acgtgtccag cggagggggc tttggaccgg ttgctgatga
     3061 cggctatggt gtgtcgtaca tccttgtggg agagaacctc atcaatttcc acatttcttc
     3121 caagttctct tgccctgaga cggattctca tcgctttgga aggcacctga aagaagcaat
     3181 gactgacatc atcactttgt ttggtctcag ttctaattcc aaaaagctcg agtacccata
     3241 cgacgtacct gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta
     3301 tgatgtccca gactacgcat gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     3361 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3421 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3481 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3541 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     3601 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3661 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3721 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3781 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3841 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3901 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3961 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4021 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     4081 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4141 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4201 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4261 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4321 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4381 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     4441 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     4501 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     4561 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4621 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     4681 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4741 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4801 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4861 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4921 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4981 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     5041 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     5101 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     5161 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     5221 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     5281 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5341 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5401 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     5461 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     5581 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5641 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5701 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5761 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5821 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5881 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5941 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6001 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6061 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6121 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6181 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6241 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6301 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6361 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6421 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6481 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     6541 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6601 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6661 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6721 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6781 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6841 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6901 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6961 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7021 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7081 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7141 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7201 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7261 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7321 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7381 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7441 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7501 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7561 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7681 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7741 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggt
//
