LOCUS pcDNA3.1-CD14(human) 6545 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031435 VERSION V031435 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..2032 /label="CD14(NM_000591)" /note="CD14(NM_000591)" /gene="CD14" polyA_signal 2112..2336 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2382..2810 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2824..3153 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3220..4014 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4188..4309 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4358..4374) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4382..4398 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4406..4436) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4451..4472 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4760..5345) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5516..6376) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6377..6481) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gagcgcgcgt cctgcttgtt gctgctgctg ctgccgctgg tgcacgtctc 961 tgcgaccacg ccagaacctt gtgagctgga cgatgaagat ttccgctgcg tctgcaactt 1021 ctccgaacct cagcccgact ggtccgaagc cttccagtgt gtgtctgcag tagaggtgga 1081 gatccatgcc ggcggtctca acctagagcc gtttctaaag cgcgtcgatg cggacgccga 1141 cccgcggcag tatgctgaca cggtcaaggc tctccgcgtg cggcggctca cagtgggagc 1201 cgcacaggtt cctgctcagc tactggtagg cgccctgcgt gtgctagcgt actcccgcct 1261 caaggaactg acgctcgagg acctaaagat aaccggcacc atgcctccgc tgcctctgga 1321 agccacagga cttgcacttt ccagcttgcg cctacgcaac gtgtcgtggg cgacagggcg 1381 ttcttggctc gccgagctgc agcagtggct caagccaggc ctcaaggtac tgagcattgc 1441 ccaagcacac tcgcctgcct tttcctgcga acaggttcgc gccttcccgg cccttaccag 1501 cctagacctg tctgacaatc ctggactggg cgaacgcgga ctgatggcgg ctctctgtcc 1561 ccacaagttc ccggccatcc agaatctagc gctgcgcaac acaggaatgg agacgcccac 1621 aggcgtgtgc gccgcactgg cggcggcagg tgtgcagccc cacagcctag acctcagcca 1681 caactcgctg cgcgccaccg taaaccctag cgctccgaga tgcatgtggt ccagcgccct 1741 gaactccctc aatctgtcgt tcgctgggct ggaacaggtg cctaaaggac tgccagccaa 1801 gctcagagtg ctcgatctca gctgcaacag actgaacagg gcgccgcagc ctgacgagct 1861 gcccgaggtg gataacctga cactggacgg gaatcccttc ctggtccctg gaactgccct 1921 cccccacgag ggctcaatga actccggcgt ggtcccagcc tgtgcacgtt cgaccctgtc 1981 ggtgggggtg tcgggaaccc tggtgctgct ccaaggggcc cggggctttg cctaagaatt 2041 ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg 2101 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 2161 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 2221 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 2281 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 2341 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 2401 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 2461 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 2521 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 2581 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 2641 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 2701 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 2761 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 2821 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 2881 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 2941 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3001 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3061 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga 3121 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt 3181 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 3241 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 3301 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 3361 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta 3421 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 3481 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 3541 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 3601 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 3661 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 3721 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc 3781 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 3841 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 3901 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 3961 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga 4021 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 4081 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 4141 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 4201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 4261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 4321 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 4381 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 4441 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 4501 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 4561 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 4621 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 4681 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 4741 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 4801 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 4861 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 4921 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 4981 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 5041 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 5101 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 5161 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 5221 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 5281 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 5341 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 5401 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 5461 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 5521 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 5581 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 5641 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 5701 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 5761 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 5821 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 5881 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 5941 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 6001 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6061 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 6121 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 6181 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 6241 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 6301 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 6361 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 6421 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 6481 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct 6541 atggt //