LOCUS       V037986                14109 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037986
VERSION     V037986
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     regulatory      913..922
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             922..987
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             991..8919
                     /label="Bahcc1(NM_001347621)"
                     /note="Bahcc1(NM_001347621)"
                     /gene="Bahcc1"
     CDS             8959..9678
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    9709..9933
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      9979..10407
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        10421..10750
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             10817..11611
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    11785..11906
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11955..11971)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11979..11995
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12003..12033)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12048..12069
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12357..12942)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13113..13973)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13974..14078)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac
      961 atcgactaca aggatgacga tgacaagctt atggatggcc gagacttcgc gccgccgccg
     1021 catttgctat cggagcgcgg gagcctgggc caccgcagcg ccgccgccgc cgcgcgcctc
     1081 gcccccgccg gccccgccgc gcaaccggcc gcgcatttcc agcccggaaa gtacttcccg
     1141 tcgccgctgc cgatggcttc gcacacagcc agcagccgtc tgatgggaaa ccccccagcc
     1201 tcctctttta tgggcagctt cctcaccagc agcctgggct cagcagcgtc ggcacacccc
     1261 agtggcccca catcctctcc ctctgagccg gcctaccgag gatcccaccc tgccacctct
     1321 cagatctggt tctcccactc ccatgaagct cctgcgtacc ccagattttc ggggagtctg
     1381 gcatctacct tcctacccgt gagccacttg gatcaccatg gaaacagcaa tgttctctat
     1441 gggcaacatc gtttctatgg aacccaaaaa gataacttct acctgcgcaa cctgccccca
     1501 cagcctacca tactacccgc caaccacaac ttccccggag tgccccgagc cacccctgcc
     1561 catcccatcg ggtcctgcag ccgggaccgc atcgaggctg cctcactgca gaagggtcct
     1621 aaggagtttg accgcttcct catgggtaaa gaggtgggca aagagaaagt tagcaaggga
     1681 gcagagggca gggagcggcc agctgtggag gaagacagtg gtaaagatcg acagaagctg
     1741 gtgccgccca tgcctgccga ggggccctgc aaggaggcag gccctgcacc ccgggggtcc
     1801 tgtgagggcc gccccaaaca cctcacctct tgcctgctca acaccaaggt actcaatgga
     1861 gacatgggca aggcctccct ggccagctgt gcaggaggca tgctgggcag gcctgggaca
     1921 ggtgtggcag cacccggacg ctgtgccaag gaggtggcag gccccgtgga gcctgggcca
     1981 gccttcagtg agtgcctgga gcggcggcag atgctgcatc atgctgtatc ctacacagta
     2041 ccctctggcc tgcctaccgg gccacccccg cccctcagca caggcccagc gggctctttc
     2101 ccctgcctgc agctgcatgc aggcccagat gggctctgcc ctctgcagga caaagtctcc
     2161 cgggacctaa aggccagcgg gcccaccttt gtgccttctg tgggacacct ggctgacaag
     2221 agccgctctt tccaagtagc agaggcctgt gccgtggcag gtgatggaaa ggaccggcac
     2281 ctggatgcgg ccatggccac cgaccatggt gcaccctatg gagtctccta cgcccaccta
     2341 aaagctgagg gcaagggcga gcggcgaccc gggggctttg aggcggccct ccacccccgg
     2401 ctgaaaggcc tggagtatct cagttcaggc cccgaggccc ccttccctgg acttcccaaa
     2461 ggcggtctgg acaaaagtgg ctactttgag ttgcccactt cacaggactg tgcccggtcc
     2521 aatcaccaag acccactggg tgggaaggcc acccaggcct gctgcacttt agacaaagtg
     2581 gccaacaaag aggcccctgc tggccctcca gtgggacaga aggtagccag gatccggcac
     2641 cagcagcact tggtggctcc tgaggtagaa tcgggaggca gcggggccga gacgaaacgc
     2701 aagtccgtgg agctggcttc tctgggttat agcggccacc acatgcctcc gtggggtgta
     2761 cagacgggcc acgacacctc catggccatc atcgaggaac gaaagggcag tgcctacctc
     2821 gacccttttg gaagtggctt acagcaggca gccctcctgt cccaggagct gcccactccg
     2881 ccagatgagg tctcggccat gaagaacctg ctgaaataca gcaaccaagc cctagtcgtg
     2941 ggccagaagg ctccgttcgt gggcctgggc agcctcaaag ccagctgcgt ccagcaggaa
     3001 gccaagtttc cagccactaa gggcccaggc ccagtggaaa gacccgactg tgcccgcagc
     3061 agggagcatg aggccccaca tggagacggg gaggtgaggc agccacccgt gggcatcgca
     3121 gtggccttgg ccaggcagaa ggacacggtc ggcaggcccg atacagccta caataccaac
     3181 agtgggcggc agggcagggc cgcccccacc ttcaaaggtg ctggaggacc acgtgccagc
     3241 catgcgctag acctggagag cgaagaggag aggtcacggg cgtgcgagga gcgcctgggt
     3301 ctgcctggac gtgagctgct gttacaagac aacaaagacc tcgtggaatt tgcccggatc
     3361 cacccttcaa gcagctgccc tggagacctg cccccccacc tcatgatgca aggcggggac
     3421 ccagcccccc acccccatcc tgcccatccc cactggctgc cccgcacccg cagtccctcc
     3481 ttgtggatgg ggggacattc ctatggtctc ggacaccccg ccctgcacca gaacctaccc
     3541 cctggcttcc ccgcatctgt gcccggctcc atgccctccg tgttccccct cccccaagat
     3601 gcagccacgc agttggtcat cttgccctcg gaacccacac cccacaccac ccctcacaca
     3661 ctcgctgaag ttatggacca ggcctccctg tggcccccca tgtatggggc ccggggccct
     3721 gcctctcaca tgcagcaccc gggccagctc cctgtgtatt ctcggtccca gctgcttcgg
     3781 cagcaagagc tctatgcact gcagcagcag cagcaacaac agcagcagca gcaacaacag
     3841 cagcaacagc agcagcaaca acaacagcag cagcagcaac agcagcagca gcaacatcgg
     3901 gccacacagg ccctggagct acagcgagtt gcccagttcc agcgcaagcc tgaggatcgc
     3961 cacatggagc tggaggaagc tgcccaggag aagaccccaa agtccaccca caagccagtt
     4021 gccttaaccc ccatggccaa gggcaccccc tcctccgcca ctgcaggcct ggtcaagcta
     4081 tcaccctgct gtcagtcgcc cactttgaag acccctgcga gttgccccac accaccacct
     4141 cggcccagcg ccccctgcac tttacccatc tgccctactg gcagcccagg gcctggctcc
     4201 aaggtgccta gtaccatgga caagagtgag gagggccagc gggctggaac caacctgacc
     4261 acgctggaac cagacctgac tccaggattg aaccccacag ctggcctgga tctctccctc
     4321 ccttcagacg tgcactcctc tgacctccaa gaccccaaaa ctatgcaaac cactacccca
     4381 gggactcggc ctgagccccc aaggacgttc ctccctgggg agccaccccc atgcagcccc
     4441 aggaacttag aagagcctgg gctgctctca agggccaggg atgccaccca ggaccttgcc
     4501 aatctacccc ctcctgttga gggaggactc ccaccaggga aggcagaaga ccccagccca
     4561 ctcgaggggt tacaagcact gaaatttgga gacctcctcg agggaggggg aactgaggct
     4621 actggccaga cgaattctac tcagggaggg atgcaaaatg agaggactgt agatcagggg
     4681 gcaccacagc cccctttggg ggctacccct caagcactag aacaggaagc agggagccca
     4741 gctgccctgg acaagcgtga aggtccacag aaggtccctg atgtggccca gctacaggag
     4801 gaagaaaccc agcttgagga gagcgggggg gactcggagg tggactgggg gactcccaac
     4861 catagccacc cacccaaagc gctgccaggc ttggatgcct tggtggctgc tactgtggac
     4921 ctaggagacc tgcctgacat cagcctgaca gaccctcaga ccccagcagc ctccgtgccc
     4981 cttagcacgg ctcctctgcc gcatagctca gggattcatg ggattgccct gctcagtgag
     5041 ctggctgacc tggagaccca gcggcagaag agtgagctgt ccatgcaaga agatgaggat
     5101 gtgctggcct tcaacctgca gcacctggcc acactggcca cagcctggtc cctggtggag
     5161 gctgctaacc tggacagccc agtcacttca ctgcaggccc cagctgctga cccagacaga
     5221 ggccccaggc tcacccccag aatgcaaatc ctgcaacgca aggacacctg ggcccctaaa
     5281 acaaagcctg tatgtcccct gaaggctgcc atcgaccggc tggacacaca ggaagtggag
     5341 atgcgcatgc agctagcaga gctgcagagg cgctacaagg agaagcaacg ggagctggct
     5401 cgcctgcagc gcaggcatga ccacgagaga gaggagagct cacggagccc tgcacggcgg
     5461 gggcctggca ggccaagaaa gcgcaaacac tcaagctcac tgcctgccct gcgtcccggg
     5521 ggccagcttg ccagaagtga cagcaagaaa gccaaggctg ttcgtgcaag cctaagtctg
     5581 ctgtgtgctg agctacgagg tgatgagccc ccaaggaagc gaagcaaact gggaaagagt
     5641 ccttacactg gcctgcagtc cgtctcctcg gagaaggtgc ggtgcaagaa gagctgtggc
     5701 caggccgagt tgccatcctc ggtggcccac aaggtggccc agctgaagcc aaaggtcaag
     5761 agcaaggggc tgcctgccgg cctcggtgcc ttccagcgca aagaggctgc cccaggtggg
     5821 cgcatccaga agaagctatc tagagccaag agcgtcacgg cgtcaggggc ggcacggcat
     5881 ccacaccccg atggggacag tggcagggag atgcacaaat tccaagccca acccgcagtg
     5941 gctgtggcac atgaggcagg cagcggctat gacagtgagg actgccaggc actcctgggg
     6001 acagaggcag ctcccagaga gcctgggctg gtgttgcacc cagggtctgg tgtggcagtg
     6061 ctgggaccct caccttcctc cgtggtcaag atggaagcca atcagaaggc caagaagaaa
     6121 aaggagaggc agggcttgct aggggcctgc cgcctgtcca gccccgaggg cgaggttaag
     6181 atcaagagac gaacagtaaa gaccaaggtg ggagccaagc tggagcgggc gccagggcgg
     6241 cggcccccgg gtgcaccagg caagaagaag gccaagggca aggtgaaaac tggcctgcgt
     6301 acagagccag gaaccgccac cagcagagac acccttttca gtcccacccg gacttttgcc
     6361 tgccgagagg agggcagcaa acttgccagt gagcgcctca agagagccac ccgcaagagc
     6421 gccatgttgc agccagtcct gaggcggaag aatggggcct tgtccatcgc tctgtcagcc
     6481 cgcaacgcca aggccatcct gggaaagagc aggaagctga cgaaggtgaa gagagaggct
     6541 gtcagcaagc agggccaggg gcgggcggtg agccggctgc tggagagctt tgctgtggag
     6601 gatgactttg agtttgacga ggatgacacc agcttctcgg atgaggaaga agaagaggaa
     6661 gaagccggtg tacagctcag cgcagagcag agcgccgccc tggcacggtc ctgcaccatc
     6721 cacaaggaag acctgcagga cggactgccc gtcctgatcc cgaaggagga cagcctgctg
     6781 tatgcgggca gtgtcaggac cctgcaaccc cccgacatct acagcatcgt cattgagggg
     6841 gagagaggca accgtcaacg tatctactcg ctggaacagc tgctgcagga ggcggttctt
     6901 gatgtccagc cacagtccag tcggtacctt ccacctggta cccgggtctg tgcctactgg
     6961 agtcagaagt cccggtgtct atatccaggc aatgtggttc gaggtgcttc aagcgatgaa
     7021 gaagacctgg actcagtgtt ggtagagttc gatgacggag acacaggcca catagctgtc
     7081 tctaacatca ggctgctgcc tccggacttc aagatccagt gtacagagcc ctcgccagcc
     7141 ctgttggtgt ccagcagctg ccggaggacc aaaaaagcag ccaatgaggg ccacccgccc
     7201 agcgaagccc ccacacccag cctgtcccct aaagtgccgg atggccctga aacttctaag
     7261 acccctggga agaaatctgg cagcaaagac aaagctggca aagtcgacct cctaacctca
     7321 ggtgccaagt cccccacggg ggcctcagac cacttcctag gccgcagagg cagccccctg
     7381 ctgagctggt cagcagtggc tcagaccaag cggaaagcgg tggctgcggc agcggcggcg
     7441 gcaggtggca aagggccggg ggtactacag aacctcttcc agctcaacgg aagcaccaag
     7501 aagctgcggg cccgggacac cctgtttccc atgcatagca tggctacccc cgtgtttggt
     7561 aacagcttcc gagctgactc cttcagcagc ctggcgagtt cctacacacc cttccttgga
     7621 ggggctgggg caggccttcc cggaggagcc cacaagctgc ttagggccaa gaaggctgaa
     7681 cgggctgaag cagaaaaggc cgggaggcgg cgggcaggcg gcgagtttct ggttaagctg
     7741 gaccatgagg gtgtgacctc ccccaaaaac aaaaactgca aggccctgct catgagcgac
     7801 aaggacttcg gacccaagct ggggcgacct cttagcaacc ccagttacgc acacccagcc
     7861 ctcattggca aggacaagaa gggtcgggcg cctgtgcatc cgctacccat ggggctggct
     7921 ctgcgcaagt acccactgcc ctgtgatagt gactgcccca gctcctactc cgatgaggac
     7981 gaggatgggc cggggctggc caccggtgtt ccctcccgat tcctcacccg cctatccatg
     8041 tcgtcgtcct cctctggctc gtccacgtct tcttcctcag gctccgtgtc cacgtccagc
     8101 ctctgttcct cagacaatga ggactcatct tatagctcag atgacgagga ccctgccctg
     8161 ctgctgcaga cctgtctcac ccgccctgtg cccgccctcc tggccccacc cgaagccctg
     8221 cgctctaagg gtagcagccc ccacgcgcac acccacgccc agcgctgctt cctgtccagg
     8281 gctggggtgg ctggtgcagg tgcaggggcc agccccagtg gtagcaaatc caagttcaag
     8341 cgcaaggagg ccctgagctt ctccaaagcc aaagagcttt ctcggaggca acggctgccc
     8401 tccgtagaaa accggccaaa gatctcagcc ttcctgcctg cccggcagct ctggaagtgg
     8461 tcaggaaacc ccacgcagag acgaggcatg aaggggaaag ccaggaaact attctacaag
     8521 gccattgtcc gaggcaagga gacactgcgc atcggggact gtgcggtctt cctttcggcc
     8581 ggccggccca acctgcccta cataggccgc atcgagagct tgtgggagtc ctggggcagc
     8641 aacatggtgg tgaaggtcaa atggttctac cacccagagg agaccaagct ggggaagcgg
     8701 cagagtgacg ggaagaacgc actgtaccag tcctgccacg aagatgagaa tgacgtgcag
     8761 accatctccc acaaatgtca ggtggtgggc cgagagcagt atgaacagat gatgcggggc
     8821 cgcaagtacc aggaccagca ggacctgtac tacttggcag gcacctatga tcctactact
     8881 gggcgcctgg tgacagctga cggcgtgccc gtcctgtgcc actcgagtgg cggtggagga
     8941 agcggcggag gtggatctat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     9001 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     9061 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     9121 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     9181 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     9241 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     9301 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     9361 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     9421 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     9481 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     9541 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     9601 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     9661 gacgagctgt acaagtaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     9721 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     9781 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     9841 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     9901 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     9961 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
    10021 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
    10081 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
    10141 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
    10201 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
    10261 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
    10321 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
    10381 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
    10441 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    10501 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    10561 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
    10621 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    10681 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    10741 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
    10801 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
    10861 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
    10921 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
    10981 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
    11041 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
    11101 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
    11161 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
    11221 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
    11281 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
    11341 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
    11401 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
    11461 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
    11521 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
    11581 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
    11641 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
    11701 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    11761 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
    11821 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    11881 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
    11941 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    12001 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    12061 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    12121 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    12181 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    12241 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    12301 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    12361 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    12421 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    12481 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    12541 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    12601 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    12661 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    12721 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    12781 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    12841 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt
    12901 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    12961 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    13021 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    13081 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    13141 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    13201 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    13261 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    13321 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    13381 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    13441 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    13501 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    13561 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    13621 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    13681 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    13741 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    13801 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    13861 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    13921 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    13981 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    14041 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    14101 cctgacgtc
//
