LOCUS       pcDNA3.1-Ar(mouse)      8084 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042972
VERSION     V042972
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3604
                     /label="Ar(NM_013476)"
                     /note="Ar(NM_013476)"
                     /gene="Ar"
     polyA_signal    3651..3875
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3921..4349
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4363..4692
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4759..5553
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5727..5848
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5897..5913)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5921..5937
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5945..5975)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5990..6011
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6299..6884)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7055..7915)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7916..8020)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gaggtgcagt tagggctggg aagggtctac ccacggcccc catccaagac
      961 ctatcgagga gcgttccaga atctgttcca gagcgtgcgc gaagcgatcc agaacccggg
     1021 ccccaggcac cctgaggccg ctaacatagc acctcccggc gcctgtttac agcagaggca
     1081 ggagactagc ccccggcggc ggcggcggca gcagcacact gaggatggtt ctcctcaagc
     1141 ccacatcaga ggccccacag gctacctggc cctggaggag gaacagcagc cttcacagca
     1201 gcaggcagcc tccgagggcc accctgagag cagctgcctc cccgagcctg gggcggccac
     1261 cgctcctggc aaggggctgc cgcagcagcc accagctcct ccagatcagg atgactcagc
     1321 tgccccatcc acgttgtccc tgctgggccc cactttccca ggcttaagca gctgctccgc
     1381 cgacattaaa gacattttga acgaggccgg caccatgcaa cttcttcagc agcagcaaca
     1441 acagcagcag caccaacagc agcaccaaca gcaccaacag cagcaggagg taatctccga
     1501 aggcagcagc gcaagagcca gggaggccac gggggctccc tcttcctcca aggatagtta
     1561 cctagggggc aattcaacca tatctgacag tgccaaggag ttgtgtaaag cagtgtctgt
     1621 gtccatggga ttgggtgtgg aagcattgga acatctgagt ccaggggaac agcttcgggg
     1681 agactgcatg tacgcgtcgc tcctgggagg tccacccgcg gtgcgtccca ctccttgtgc
     1741 gccgctgccc gaatgcaaag gtcttcccct ggacgaaggc ccaggcaaaa gcactgaaga
     1801 gactgctgag tattcctctt tcaagggagg ttacgccaaa ggattggaag gtgagagctt
     1861 ggggtgctct ggcagcagtg aagcaggtag ctctgggaca cttgagatcc cgtcctctct
     1921 gtctctgtat aaatctggag cactagacga ggcagcagca taccagaatc gcgactacta
     1981 caactttccg ctggctctgt ccgggccgcc gcaccccccg ccccctaccc atccacacgc
     2041 ccgtatcaag ctggagaacc cattggacta cggcagcgcc tgggctgcgg cggcagcgca
     2101 atgccgctat ggggacttgg gtagtctaca tggagggagt gtagccgggc ccagcactgg
     2161 atcgccccca gccaccacct cttcttcctg gcatactctc ttcacagctg aagaaggcca
     2221 attatatggg ccaggaggcg ggggcggcag cagcagccca agcgatgccg ggcctgtagc
     2281 cccctatggc tacactcggc cccctcaggg gctgacaagc caggagagtg actactctgc
     2341 ctccgaagtg tggtatcctg gtggagttgt gaacagagta ccctatccca gtcccaattg
     2401 tgtcaaaagt gaaatgggac cttggatgga gaactactcc ggaccttatg gggacatgcg
     2461 tttggacagt accagggacc atgttttacc catcgactat tactttccac cccagaagac
     2521 ctgcctgatc tgtggagatg aagcttctgg ctgtcactac ggagctctca cttgtggcag
     2581 ctgcaaggtc ttcttcaaaa gagccgctga agggaaacag aagtatctat gtgccagcag
     2641 aaacgattgt accattgata aatttcggag gaaaaattgc ccatcttgtc gtctccggaa
     2701 atgttatgaa gcagggatga ctctgggagc tcgtaagctg aagaaacttg gaaatctaaa
     2761 actacaggag gaaggagaaa actccaatgc tggcagcccc actgaggacc catcccagaa
     2821 gatgactgta tcacacattg aaggctatga atgtcagcct atctttctta acgtcctgga
     2881 agccattgag ccaggagtgg tgtgtgccgg acatgacaac aaccaaccag attcctttgc
     2941 tgccttgtta tctagcctca atgagcttgg agagaggcag cttgtgcatg tggtcaagtg
     3001 ggccaaggcc ttgcctggct tccgcaactt gcatgtggat gaccagatgg cggtcattca
     3061 gtattcctgg atgggactga tggtatttgc catgggttgg cggtccttca ctaatgtcaa
     3121 ctccaggatg ctctactttg cacctgactt ggttttcaat gagtaccgca tgcacaagtc
     3181 tcggatgtac agccagtgtg tgaggatgag gcacctgtct caagagtttg gatggctcca
     3241 aataaccccc caggaattcc tgtgcatgaa agcactgctg ctcttcagca ttattccagt
     3301 ggatgggctg aaaaatcaaa aattctttga tgaacttcga atgaactaca tcaaggaact
     3361 cgatcgcatc attgcatgca aaagaaagaa tcccacatcc tgctcaaggc gcttctacca
     3421 gctcaccaag ctcctggatt ctgtgcagcc tattgcaaga gagctgcatc agttcacttt
     3481 tgacctgcta atcaagtccc atatggtgag cgtggacttt cctgaaatga tggcagagat
     3541 catctctgtg caagtgccca agatcctttc tgggaaagtc aagcccatct atttccacac
     3601 acagtgactc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     3661 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3721 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     3781 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3841 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     3901 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3961 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4021 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4081 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4141 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4201 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4261 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4321 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     4381 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4441 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4501 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4561 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4621 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4681 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     4741 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4801 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4861 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4921 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     4981 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5041 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5101 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5161 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5221 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5281 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5341 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5401 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5461 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5521 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5581 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     5641 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5701 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     5761 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5821 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     5881 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5941 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6001 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6061 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6721 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt
     6841 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6901 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6961 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7021 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7081 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7141 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7201 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7261 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7321 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7381 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7441 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7501 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7561 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7621 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7681 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7741 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7801 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7861 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7921 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7981 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8041 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggt
//
