LOCUS       V027429                 8038 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027429
VERSION     V027429
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3522
                     /label="Akap3(NM_009650)"
                     /note="Akap3(NM_009650)"
                     /gene="Akap3"
     CDS             3529..3558
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    3605..3829
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3875..4303
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4317..4646
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4713..5507
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5681..5802
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5851..5867)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5875..5891
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5899..5929)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5944..5965
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6253..6838)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7009..7869)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7870..7974)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggcggata gggttgactg gttacaaagt
      961 caaagtggcg tttgcaaagt tggtgtctat tcacctggag acaaccaaca ccaagactgg
     1021 aaaatggaca catcaacaga tcctgtccga gtgctcagct ggctccgcaa agacctggag
     1081 aaaagtacag cgggcttcca ggactcgagg ttcaagcctg gagagtcatc gtttgtggag
     1141 gaagtggctt acccagtgga ccaacgaaaa ggtttctgtg ttgattatta caataccacc
     1201 aacaagggca gtccaggaag attgcatttt gagatgtctc acaaggagaa cccttcccag
     1261 ggcctcattt cccatgttgg taatgggggt tccatagacg aagtttcctt ctatgccaac
     1321 cgcctcacaa acctagtgat cgccatggcc cgaaaggaga tcaatgagaa gatccacggc
     1381 gctgaaaaca aatgtgtcca tcagtcattg tatatggggg atgagcccac accccacaaa
     1441 agcttgagta cagtggcctc tgagctcgtg aacgagacag tcaccgcatg ttccaagaac
     1501 atttccagtg acaaagctcc cggctctgga gacagggcct cggggtcgtc acaggcccct
     1561 ggtctaagat acacgagcac tctgaagatc aaggagagta caaaggaagg caagtgtcca
     1621 gacgacaagc ccggcactaa gaagtctttc ttctataagg aagtgtttga gtcccggaat
     1681 gcaggagatg ccaaggaggg cgggaggtcc ttaccaggag atcaaaaact gttcaggacc
     1741 agccccgaca acaggcctga tgacttttca aactctatca gtcaagggat catgacctac
     1801 gccaacagcg tggtgtctga catgatggtc tccatcatga agacgctgaa gatccaggtg
     1861 aaggacacaa ccatcgccac gattctgctg aagaaggtgc tgatgaagca cgcaaaggag
     1921 gttgtctccg acctcatcga ctccttcatg aagaacctcc acggcgtcac gggaagcctc
     1981 atgactgaca cagactttgt ctcggccgtg aaacgaagtt ttttttctca tggaagccaa
     2041 aaggccacag atatcatgga tgccatgctg ggcaagctat acaatgtgat gtttgccaag
     2101 aaattccctg agaacatccg gagagccagg gacaagtcgg agagttactc ccttatctcc
     2161 acgaaatcac gggctggtga cccaaagctc tcaaatttga actttgcgat gaagtcagaa
     2221 tcaaagctga aagaaaattt gttttctaca tgcaaactag agaaagagaa gacgtgtgcc
     2281 gaaactctgg gtgagcatat tattaaggag ggactgcaca tgtggcacaa gagtcagcaa
     2341 aaatctcctg gcttggagcg tgccgcaaaa ctgggtaacg ctccacagga ggtctccttt
     2401 gagtgcccag atccttgtga ggcaaaccct cctcaccaac ctcagccacc agagaatttt
     2461 gcaaatttta tgtgtgactc agactcctgg gccaaggacc tgattgtatc cgccctgctt
     2521 ctgattcagt atcacctggc acagggagga aagatggatg ctcagagctt cctggaagct
     2581 gctgccagca ccaattttcc caccaacaag ccacctcctc cttctcctgt agttcaggat
     2641 gagtgcaaac ttaagtctcc tccccacaag atatgtgacc aagaacaaac agaaaagaaa
     2701 gatctgatga gtgtcatctt caattttatc cggaacttac tcagcgagac catattcaag
     2761 agtagccgta actgtgaatc caatgtgcat gagcagaaca ctcaggaaga agagatacac
     2821 ccgtgtgaaa ggcctaagac tccatgtgaa aggcctatta ccccgcctgc cccgaaattc
     2881 tgtgaggatg aggaggccac tggtggtgcc ttatctgggc taaccaagat ggttgccaac
     2941 cagctagaca actgtatgaa tgggcagatg gtggagcacc tgatggactc ggtgatgaag
     3001 ttatgcctca ttattgccaa gtcctgtgac tctcccctgt cggagctggg agaggaaaag
     3061 tgtggggatg ccagccggcc aaattctgcc ttcccagata acttatatga gtgcctacca
     3121 gtcaagggca cagggacagc tgaagccctc ctgcagaacg cctacctcac catccataat
     3181 gaactgagag gtttgtcagg acagcccccc gagggctgtg aaatccccaa ggtgatcgtc
     3241 agcaaccaca atctggctga caccgttcag aacaagcaac tgcaagctgt ccttcagtgg
     3301 gtggctgcct cagagctcaa tgtccctatt ttgtactttg ctggtgacga tgaaggaatc
     3361 caggagaagc tgcttcagct ctcagccact gccgtggaga aaggccgcag cgtcggggag
     3421 gttctgcagt cggtgctgag gtacgagaag gagcgacagc tggatgaagc agtgggaaat
     3481 gtcacgcggc tgcagctgct ggactggctg atggcaaacc tggatatcga gcagaagctg
     3541 atctcagagg aggacctgta gctcgagtct agagggcccg tttaaacccg ctgatcagcc
     3601 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3661 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3721 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     3781 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     3841 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     3901 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3961 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4021 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4081 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4141 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4201 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4261 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     4321 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4381 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4441 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4501 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4561 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     4621 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     4681 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     4741 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4801 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4861 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     4921 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4981 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5041 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     5101 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5161 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5221 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5281 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5341 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5401 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5461 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     5521 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5581 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5641 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     5701 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5761 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     5821 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5941 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6001 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6061 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6241 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6781 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7861 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7921 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7981 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggt
//
