LOCUS V041814 7252 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041814 VERSION V041814 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..2742 /label="ARSD(NM_001669)" /note="ARSD(NM_001669)" /gene="ARSD" CDS 2749..2814 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2852..3076 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3122..3550 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3564..3893 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3960..4754 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4928..5049 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5098..5114) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5122..5138 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5146..5176) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5191..5212 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5500..6085) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6256..7116) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7117..7221) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatgcgat ccgccgcgcg gaggggacgc gccgcgcccg ccgccaggga ctctttgccg 1021 gtgctactgt ttttatgctt gcttctgaag acgtgtgaac ctaaaactgc aaatgccttt 1081 aaaccaaata tcctactgat catggcggat gatctaggca ctggggatct cggttgctac 1141 gggaacaata cactgagaac gccgaatatt gaccagcttg cagaggaagg tgtgaggctc 1201 actcagcacc tggcggccgc cccgctctgc accccaagcc gagctgcatt cctcacaggg 1261 agacattcct tcagatcagg catggacgcc agcaatggat accgggccct tcagtggaac 1321 gcaggctcag gtggactccc tgagaacgaa accacttttg caagaatctt gcagcagcat 1381 ggctatgcaa ccggcctcat aggaaaatgg caccagggtg tgaattgtgc atcccgcggg 1441 gatcactgcc accaccccct gaaccacgga tttgactatt tctacggcat gcccttcacg 1501 ctcacaaacg actgtgaccc aggcaggccc cccgaagtgg acgccgccct gagggcgcag 1561 ctctggggtt acacccagtt cctggcgctg gggattctca ccctggctgc cggccagacc 1621 tgcggtttct tctctgtctc cgcgagagca gtcaccggca tggccggcgt gggctgcctg 1681 tttttcatct cttggtactc ctccttcggg tttgtgcgac gctggaactg tatcctgatg 1741 agaaaccatg acgtcacgga gcaacccatg gttctggaga aaacagcgag tcttatgcta 1801 aaggaagctg tttcctatat tgaaagacac aagcatgggc catttctcct cttcctttct 1861 ttgctgcatg tgcacattcc ccttgtgacc acgagtgcat tcctggggaa aagtcagcat 1921 ggcttatatg gtgataatgt ggaggagatg gactggctca taggtaaggt tcttaatgcc 1981 atcgaagaca atggtttaaa gaactcaaca ttcacgtatt tcacctctga ccatggagga 2041 catttagagg caagagatgg acacagccag ttagggggat ggaacggaat ttacaaaggt 2101 gggaagggca tgggaggatg ggaaggtggg atccgcgtgc ccgggatctt ccactggccg 2161 ggggtgctcc cggccggccg agtgattgga gagcccacga gcctgatgga cgtgttccct 2221 actgtggtcc agctggtggg tggcgaggtg ccccaggaca gggtgattga tggccacagc 2281 ctggtaccct tgctgcaggg agctgaggca cgctcggcac atgagttcct gtttcattac 2341 tgtgggcagc atcttcacgc agcacgctgg caccagaagg acagtggaag cgtctggaag 2401 gttcattaca cgaccccgca gttccacccc gagggagcgg gggcctgcta cggccgaggc 2461 gtctgcccat gctccgggga gggcgtgacc catcacagac cccctttgct ctttgacctc 2521 tccagggacc cctccgaggc acggcccctg acccccgact ccgagcccct gtaccacgcc 2581 gtgatagcaa gggtaggtgc cgcggtgtcg gagcatcggc agaccctgag tcctgtgccc 2641 cagcagtttt ccatgagcaa catcctgtgg aagccgtggc tgcagccgtg ctgcggacat 2701 ttcccgttct gttcatgcca cgaggatggg gatggcaccc ccctcgagga ctacaaagac 2761 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagtga 2821 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 2881 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 2941 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 3001 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 3061 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 3121 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 3181 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 3241 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 3301 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 3361 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 3421 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 3481 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 3541 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 3601 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 3661 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 3721 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 3781 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 3841 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 3901 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca 3961 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 4021 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 4081 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 4141 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 4201 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 4261 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 4321 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 4381 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 4441 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 4501 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 4561 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 4621 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 4681 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 4741 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 4801 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 4861 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 4921 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 4981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 5041 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 5101 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 5161 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 5221 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 5281 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 5341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 5401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 5461 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 5521 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 5581 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 5641 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 5701 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 5761 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 5821 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 5881 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 5941 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 6001 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat 6061 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 6121 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 6181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 6241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 6301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 6361 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 6421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 6481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 6541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 6601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 6661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 6721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 6781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 6841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 6901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 6961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 7021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 7081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 7141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 7201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //