LOCUS V021447 6949 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021447 VERSION V021447 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 872..1678 /label="AQP1(NM_198098)" /note="AQP1(NM_198098)" /gene="AQP1" CDS 1706..2416 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" polyA_signal 2516..2740 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2786..3214 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3228..3557 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3624..4418 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4592..4713 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4762..4778) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4786..4802 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4810..4840) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4855..4876 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5164..5749) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5920..6780) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6781..6885) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagccac catggccagc gagttcaaga agaagctctt 901 ctggagggca gtggtggccg agttcctggc cacgaccctc tttgtcttca tcagcatcgg 961 ttctgccctg ggcttcaaat acccggtggg gaacaaccag acggcggtcc aggacaacgt 1021 gaaggtgtcg ctggccttcg ggctgagcat cgccacgctg gcgcagagtg tgggccacat 1081 cagcggcgcc cacctcaacc cggctgtcac actggggctg ctgctcagct gccagatcag 1141 catcttccgt gccctcatgt acatcatcgc ccagtgcgtg ggggccatcg tcgccaccgc 1201 catcctctca ggcatcacct cctccctgac tgggaactcg cttggccgca atgacctggc 1261 tgatggtgtg aactcgggcc agggcctggg catcgagatc atcgggaccc tccagctggt 1321 gctatgcgtg ctggctacta ccgaccggag gcgccgtgac cttggtggct cagcccccct 1381 tgccatcggc ctctctgtag cccttggaca cctcctggct attgactaca ctggctgtgg 1441 gattaaccct gctcggtcct ttggctccgc ggtgatcaca cacaacttca gcaaccactg 1501 gattttctgg gtggggccat tcatcggggg agccctggct gtactcatct acgacttcat 1561 cctggcccca cgcagcagtg acctcacaga ccgcgtgaag gtgtggacca gcggccaggt 1621 ggaggagtat gacctggatg ccgacgacat caactccagg gtggagatga agcccaaagg 1681 aggaggggga agcggtaccg ccaccatggt gagcaagggc gaggaggata acatggccat 1741 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt 1801 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 1861 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta 1921 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt 1981 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt 2041 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac 2101 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc 2161 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct 2221 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt 2281 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga 2341 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 2401 cgagctgtac aagtagggat ccactagtcc agtgtggtgg aattctgcag atatccagca 2461 cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg 2521 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 2581 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 2641 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 2701 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 2761 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 2821 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 2881 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 2941 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 3001 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 3061 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 3121 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 3181 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 3241 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 3301 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3361 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 3421 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 3481 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 3541 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 3601 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 3661 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 3721 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 3781 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 3841 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 3901 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 3961 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 4021 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 4081 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 4141 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 4201 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 4261 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 4321 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 4381 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 4441 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 4501 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 4561 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 4621 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 4681 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 4741 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 4801 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 4861 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 4921 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4981 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 5041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 5101 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 5161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 5221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 5281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 5341 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 5401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 5461 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 5521 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 5581 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 5641 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 5701 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 5761 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 5821 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 5881 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 5941 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 6001 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6061 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6121 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 6181 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 6241 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 6301 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 6361 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 6421 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 6481 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 6541 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 6601 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 6661 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 6721 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 6781 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 6841 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 6901 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt //