LOCUS V037303 6608 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037303 VERSION V037303 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..2095 /label="APOA4(NM_000482)" /note="APOA4(NM_000482)" /gene="APOA4" CDS 2111..2128 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" polyA_signal 2175..2399 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2445..2873 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2887..3216 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3283..4077 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4251..4372 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4421..4437) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4445..4461 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4469..4499) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4514..4535 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4823..5408) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5579..6439) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6440..6544) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg ttcctgaagg ccgtggtcct gaccctggcc ctggtggctg tcgccggagc 961 cagggctgag gtcagtgctg accaggtggc cacggtgatg tgggactact tcagccagct 1021 gagcaacaat gccaaggagg ccgtggaaca tctccagaaa tctgaactca cccagcaact 1081 caatgccctc ttccaggaca aacttggaga agtgaacact tacgcaggtg acctgcagaa 1141 gaagctggtg ccctttgcca ccgagctgca tgaacgcctg gccaaggact cggagaaact 1201 gaaggaggag attgggaagg agctggagga gctgagggcc cggctgctgc cccatgccaa 1261 tgaggtgagc cagaagatcg gggacaacct gcgagagctt cagcagcgcc tggagcccta 1321 cgcggaccag ctgcgcaccc aggtcagcac gcaggccgag cagctgcggc gccagctgac 1381 cccctacgca cagcgcatgg agagagtgct gcgggagaac gccgacagcc tgcaggcctc 1441 gctgaggccc cacgccgacg agctcaaggc caagatcgac cagaacgtgg aggagctcaa 1501 gggacgcctt acgccctacg ctgacgaatt caaagtcaag attgaccaga ccgtggagga 1561 gctgcgccgc agcctggctc cctatgctca ggacacgcag gagaagctca accaccagct 1621 tgagggcctg accttccaga tgaagaagaa cgccgaggag ctcaaggcca ggatctcggc 1681 cagtgccgag gagctgcggc agaggctggc gcccttggcc gaggacgtgc gtggcaacct 1741 gaggggcaac accgaggggc tgcagaagtc actggcagag ctgggtgggc acctggacca 1801 gcaggtggag gagttccgac gccgggtgga gccctacggg gaaaacttca acaaagccct 1861 ggtgcagcag atggaacagc tcaggcagaa actgggcccc catgcggggg acgtggaagg 1921 ccacttgagc ttcctggaga aggacctgag ggacaaggtc aactccttct tcagcacctt 1981 caaggagaaa gagagccagg acaagactct ctccctccct gagctggagc aacagcagga 2041 acagcagcag gagcagcagc aggagcaggt gcagatgctg gcccctttgg agagcggtgg 2101 aggcggatca catcaccatc atcaccattg actcgagtct agagggcccg tttaaacccg 2161 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 2221 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 2281 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 2341 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 2401 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 2461 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 2521 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 2581 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 2641 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 2701 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 2761 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 2821 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 2881 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 2941 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 3001 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 3061 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 3121 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 3181 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 3241 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 3301 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 3361 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 3421 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 3481 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 3541 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 3601 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 3661 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 3721 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 3781 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 3841 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 3901 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 3961 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 4021 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 4081 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 4141 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 4201 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 4261 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 4321 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 4381 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 4441 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 4501 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 4561 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 4621 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4681 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4741 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4801 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4861 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4921 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4981 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 5041 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 5101 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5161 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5221 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 5281 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5341 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 5401 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 5461 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 5521 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 5581 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 5641 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 5701 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 5761 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 5821 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 5881 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 5941 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 6001 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 6061 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 6121 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 6181 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 6241 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 6301 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 6361 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 6421 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 6481 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 6541 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 6601 cctatggt //