LOCUS V022903 9368 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022903 VERSION V022903 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..1624 /codon_start=1 /note="mammalian codon-optimized" /product="enhanced YFP" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EYFP" CDS 1631..4882 /label="AMOT(NM_001113490)" /note="AMOT(NM_001113490)" /gene="AMOT" polyA_signal 4935..5159 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 5205..5633 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5647..5976 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6043..6837 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 7011..7132 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7181..7197) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7205..7221 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7229..7259) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7274..7295 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7583..8168) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8339..9199) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9200..9304) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 961 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 1021 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 1081 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca 1141 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 1201 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 1261 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 1321 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 1381 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 1441 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 1501 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca 1561 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 1621 caaggaattc atgagaaatt ctgaagaaca gccaagtgga gggaccacgg tattgcagcg 1681 tttgctacaa gagcagcttc gctatggcaa tcctagtgag aatcgcagcc tgcttgccat 1741 acaccagcaa gccacaggga atggccctcc tttccccagt ggcagtggga acccgggccc 1801 tcagagtgat gtgttgagtc cccaagacca ccaccaacag cttgtggctc atgctgctcg 1861 acaagaaccc caggggcagg aaatccagtc agaaaacctc atcatggaga agcagctgtc 1921 tcctcgaatg caaaataatg aagaactccc gacctatgaa gaagccaagg tccagtccca 1981 gtactttcgg ggccaacagc atgccagtgt tggagctgcc ttctatgtca ctggagtcac 2041 caaccagaag atgaggactg agggacgccc atcagttcag cggctcaatc ctggaaagat 2101 gcaccaagat gagggactca gagaccttaa gcaagggcat gtccgttcct tgagtgaacg 2161 actaatgcag atgtcactgg ccaccagtgg agttaaggcc catccacctg ttaccagtgc 2221 tcccctctcc ccaccacaac ccaatgacct ctacaagaat cccacaagtt ccagtgaatt 2281 ctacaaggcc caagggccac ttcctaacca gcatagcctg aagggcatgg aacaccgagg 2341 ccccccacca gaatatccct tcaagggcat gccaccccaa tctgtagtgt gcaagcccca 2401 agagccaggg cacttctata gtgagcatcg cctgaaccag ccagggagaa cagaggggca 2461 actgatgagg tatcagcatc cccctgagta tggagcagcc aggccagcgc aggacatctc 2521 attgccattg tcagccagga actcgcagcc tcacagccct acttcttctc tgacctctgg 2581 ggggtccttg cccttgctac aatctccacc atccactaga ttgtctcctg cccgacaccc 2641 cttggtccca aaccagggag accattcagc tcacctgcct aggccgcagc agcatttcct 2701 tcctaatcag gctcaccagg gggatcatta ccgtctctcc caacctggcc tgagtcagca 2761 gcagcagcaa cagcagcagc agcaccatca tcaccatcac caccaacaac agcagcagca 2821 gcagccacag cagcagccag gagaagccta ttcagctatg cctcgggctc agccatcctc 2881 tgcttcttat cagccagtgc cagcagaccc ttttgccatt gtttccagag cccagcagat 2941 ggttgagatc ctctcagacg agaaccggaa cttgaggcaa gagttggaag gatgctatga 3001 gaaggtggca agactgcaga aggtggagac agaaatccag cgcgtctcgg aggcatatga 3061 gaacctcgtg aagtcatcct ccaaaagaga ggccctagag aaagccatga gaaacaagct 3121 agagggcgag attcggagga tgcatgattt caacagggat ctgagagagc gtctagagac 3181 tgccaacaag cagcttgcag agaaggaata tgaggggtca gaggacacca gaaaaaccat 3241 ctcgcagctc tttgcaaaaa ataaagaaag ccagcgtgag aaggagaagc tggaagcgga 3301 gctggccact gcccgttcta ccaatgagga ccaaagacga cacatcgaaa tccgagatca 3361 ggccctgagt aatgcccagg ccaaggtggt aaagctggaa gaagagctga aaaagaagca 3421 agtgtacgtt gacaaggtgg agaagatgca gcaggccctt gtacagctcc aggcagcatg 3481 tgaaaaacgt gagcagctag agcaccgtct ccggacacga ctggagaggg aactggaatc 3541 cctgagaatc cagcagcgtc agggcaactg tcagcccacc aacgtttcag aatacaatgc 3601 tgctgcactg atggagctcc ttcgggagaa agaggagagg attctggctc tggaagctga 3661 tatgacaaag tgggagcaga aatatttgga ggagaatgtg atgagacatt ttgctctgga 3721 tgctgctgca actgtggctg ctcagaggga cacaacagtc atcagtcact ctcctaacac 3781 cagctatgac acagctctag aagctcgcat ccagaaagag gaggaagaaa tcttgatggc 3841 caataagcgt tgccttgaca tggagggcag gattaagacc ctccatgccc agattattga 3901 gaaggatgcc atgatcaaag tactccagca gcgttcccgg aaggagccga gcaagacaga 3961 gcagctgtcg tgcatgcggc cagcgaagtc tctgatgtcc atttccaatg ctggatcagg 4021 cttgctctcc cactcatcca ccctgactgg ctcccccatc atggaagaaa agcgagacga 4081 caagagctgg aaggggagcc taggcattct cctgggtgga gactaccgtg ctgaatatgt 4141 cccttccaca ccctcgcctg tgccaccctc gactcccctg ctctcggctc actccaagac 4201 aggcagccga gactgcagta cccaaactga acgtgggacg gaatcgaaca aaactgcagc 4261 tgttgctccc atctctgttc ctgctccagt tgctgctgcc gccactgctg ccgccatcac 4321 tgccactgct gccaccatca ccaccaccat ggtagctgct gctccagttg ctgttgctgc 4381 tgctgctgct ccagctgctg ctgctgcccc gtctccagcc actgccgctg ctactgctgc 4441 tgctgtttct ccagctgctg ctggtcagat tccagctgct gcctctgttg cctcagctgc 4501 tgccgttgct ccttctgctg ctgctgctgc tgctgttcag gttgctccag ctgctccggc 4561 tccagttcca gctccggctc tggttccggt tccagctcca gcagcggctc aggcttctgc 4621 tcctgctcag actcaggcac caacttcagc tccggctgtg gctccaactc cagctccaac 4681 tccaactcca gctgtggctc aggctgaggt tcctgcaagt ccagctaccg gtcctggacc 4741 acatcgtttg tctataccaa gtttgacctg caatccagac aaaacagatg ggcctgtgtt 4801 ccactccaat actctggaaa gaaaaactcc cattcagatc ctgggacaag agcctgatgc 4861 agagatggtg gaatatctca tcaccggtta gctcgagtct agagggcccg tttaaacccg 4921 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 4981 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 5041 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 5101 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 5161 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 5221 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 5281 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 5341 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 5401 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 5461 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 5521 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 5581 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 5641 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 5701 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 5761 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 5821 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 5881 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 5941 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 6001 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 6061 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 6121 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 6181 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 6241 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 6301 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 6361 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 6421 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 6481 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 6541 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 6601 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 6661 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 6721 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 6781 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 6841 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 6901 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 6961 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 7021 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 7081 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 7141 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 7201 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 7261 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 7321 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 7381 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7441 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7501 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7561 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 7621 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 7681 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 7741 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 7801 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 7861 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 7921 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 7981 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 8041 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 8101 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 8221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 8281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 8341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 8401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 8461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 8581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 8641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 8701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 8761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 8821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 8881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 8941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 9241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 9301 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 9361 cctatggt //