LOCUS V031376 7006 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031376 VERSION V031376 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..2496 /label="ALDH7A1(NM_001201377)" /note="ALDH7A1(NM_001201377)" /gene="ALDH7A1" CDS 2503..2568 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2606..2830 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2876..3304 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3318..3647 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3714..4508 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4682..4803 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4852..4868) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4876..4892 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4900..4930) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4945..4966 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5254..5839) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6010..6870) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6871..6975) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatgtcca ctctcctcat caatcagccc cagtatgcgt ggctgaaaga gctggggctc 1021 cgcgaggaaa acgagggcgt gtataatgga agctggggag gccggggaga ggttattacg 1081 acctattgcc ctgctaacaa cgagccaata gcaagagtcc gacaggccag tgtggcagac 1141 tatgaagaaa ctgtaaagaa agcaagagaa gcatggaaaa tctgggcaga tattcctgct 1201 ccaaaacgag gagaaatagt aagacagatt ggcgatgcct tgcgggagaa gatccaagta 1261 ctaggaagct tggtgtcttt ggagatgggg aaaatcttag tggaaggtgt gggtgaagtt 1321 caggagtatg tggatatctg tgactatgct gttggtttat caaggatgat tggaggacct 1381 atcttgcctt ctgaaagatc tggccatgca ctgattgagc agtggaatcc cgtaggcctg 1441 gttggaatca tcacggcatt caatttccct gtggcagtgt atggttggaa caacgccatc 1501 gccatgatct gtggaaatgt ctgcctctgg aaaggagctc caaccacttc cctcattagt 1561 gtggctgtca caaagataat agccaaggtt ctggaggaca acaagctgcc tggtgcaatt 1621 tgttccttga cttgtggtgg agcagatatt ggcacagcaa tggccaaaga tgaacgagtg 1681 aacctgctgt ccttcactgg gagcactcag gtgggaaaac aggtgggcct gatggtgcag 1741 gagaggtttg ggagaagtct gttggaactt ggaggaaaca atgccattat tgcctttgaa 1801 gatgcagacc tcagcttagt tgttccatca gctctcttcg ctgctgtggg aacagctggc 1861 cagaggtgta ccactgcgag gcgactgttt atacatgaaa gcatccatga tgaggttgta 1921 aacagactta aaaaggccta tgcacagatc cgagttggga acccatggga ccctaatgtt 1981 ctctatgggc cactccacac caagcaggca gtgagcatgt ttcttggagc agtggaagaa 2041 gcaaagaaag aaggtggcac agtggtctat gggggcaagg ttatggatcg ccctggaaat 2101 tatgtagaac cgacaattgt gacaggtctt ggccacgatg cgtccattgc acacacagag 2161 acttttgctc cgattctcta tgtctttaaa ttcaagaatg aagaagaggt ctttgcatgg 2221 aataatgaag taaaacaggg actttcaagt agcatcttta ccaaagatct gggcagaatc 2281 tttcgctggc ttggacctaa aggatcagac tgtggcattg taaatgtcaa cattccaaca 2341 agtggggctg agattggagg tgcctttgga ggagaaaagc acactggtgg tggcagggag 2401 tctggcagtg atgcctggaa acagtacatg agaaggtcta cttgtactat caactacagt 2461 aaagaccttc ctctggccca aggaatcaag tttcagctcg aggactacaa agaccatgac 2521 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gtgagggccc 2581 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 2641 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 2701 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 2761 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 2821 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 2881 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 2941 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 3001 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 3061 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 3121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 3181 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 3241 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 3301 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 3361 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 3421 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 3481 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 3541 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 3601 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 3661 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg 3721 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 3781 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 3841 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg 3901 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 3961 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 4021 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 4081 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 4141 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 4201 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg 4261 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 4321 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 4381 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 4441 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 4501 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc 4561 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 4621 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 4681 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 4741 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 4801 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 4861 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 4921 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 4981 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 5041 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 5101 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 5161 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 5221 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 5281 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 5341 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 5401 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 5461 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 5521 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 5581 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 5641 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 5701 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 5761 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg 5821 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 5881 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 5941 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 6001 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 6061 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 6121 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 6181 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6241 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6301 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 6361 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 6421 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 6481 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 6541 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 6601 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 6661 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 6721 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 6781 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 6841 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 6901 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 6961 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc //