LOCUS V041124 8502 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041124 VERSION V041124 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 887..3271 /label="ALDH18A1(NM_002860)" /note="ALDH18A1(NM_002860)" /gene="ALDH18A1" CDS 3308..4018 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" polyA_signal 4069..4293 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4339..4767 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4781..5110 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5177..5971 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6145..6266 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6315..6331) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6339..6355 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6363..6393) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6408..6429 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6717..7302) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7473..8333) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8334..8438) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcaac ttaaggatcc gccaccatgt tgagtcaagt 901 ttaccgctgt gggttccagc ccttcaacca acatcttctg ccctgggtca agtgtacaac 961 cgtcttcaga tctcattgta tccagccttc agtcatcaga catgttcgtt cttggagcaa 1021 catcccgttt atcactgtac ccctcagtcg tacacatggc aagtccttcg cccaccgcag 1081 tgagctgaag catgccaaga gaatcgtggt gaagctcggc agtgccgtgg tgacccgagg 1141 ggatgaatgt ggcctggccc tggggcgctt ggcatctatt gttgagcagg tatcagtgct 1201 gcagaatcag ggcagagaga tgatgctggt gaccagtgga gccgtagcct ttggcaaaca 1261 acgcttgcgc catgagatcc ttctgtctca gagcgtgcgg caggccctcc actcggggca 1321 gaaccagctg aaagaaatgg caattccagt cttagaggca cgagcctgtg cagctgccgg 1381 acagagtggg ctgatggcct tgtatgaggc tatgtttacc cagtacagca tctgtgctgc 1441 ccagattttg gtgaccaatt tggatttcca tgatgagcag aagcgccgga acctcaatgg 1501 aacacttcat gaactcctta gaatgaacat tgtccccatt gtcaacacaa atgatgctgt 1561 tgtcccccca gctgagccca acagtgacct gcagggggta aatgttatta gtgttaaaga 1621 taatgatagc ctggctgccc gactggctgt ggaaatgaaa actgatctct tgattgttct 1681 ttcagatgta gaaggccttt ttgacagccc cccaggttca gatgatgcaa agcttattga 1741 tatattttat cccggagatc agcagtctgt gacatttgga accaagtcta gagtgggaat 1801 gggtggcatg gaagccaagg tgaaagcagc cctctgggct ttgcaaggtg gcacttctgt 1861 tgttattgcc aatggaaccc acccaaaggt gtctgggcac gtcatcacag acattgtgga 1921 ggggaagaaa gttggtacct tcttttcaga agtaaagcct gcaggcccta ctgttgagca 1981 gcagggagaa atggcgcgat ctggaggaag gatgttggcc accttggaac ctgagcagag 2041 agcagaaatt atccatcatc tggctgatct gttgacggac cagcgtgatg agatcctgtt 2101 agccaacaaa aaagacttgg aggaggcaga ggggagactt gcagctcctc tgctgaaacg 2161 tttaagcctc tccacatcca aattgaacag cctggccatc ggtctgcgac agatcgcagc 2221 ctcctcccag gacagcgtgg gacgtgtttt gcgccgcacc cgaatcgcca aaaacttgga 2281 actggaacaa gtgactgtcc caattggagt tctgctggtg atctttgaat ctcgtcctga 2341 ctgtctaccc caggtggcag ctttggctat cgcaagtggc aatggcttgt tactcaaagg 2401 agggaaggag gctgcacaca gcaaccggat tctccacctc ctgacccagg aggctctctc 2461 aatccatgga gtcaaggagg ccgtgcaact ggtgaatacc agagaagaag ttgaagatct 2521 ttgccgccta gacaaaatga tagatctgat cattccacgt ggctcttccc agctggtcag 2581 agacatccag aaagctgcta aggggattcc agtgatgggg cacagcgaag ggatctgtca 2641 catgtatgtg gattccgagg ccagtgttga taaggtcacc aggctagtca gagactctaa 2701 atgtgaatat ccagctgcct gtaatgcttt ggagactttg ttaatccacc gggatctgct 2761 caggacacca ttatttgacc agatcattga tatgctgaga gtggaacagg taaaaattca 2821 tgcaggcccc aaatttgcct cctatctgac cttcagcccc tccgaagtga agtcactccg 2881 aactgagtat ggggacctgg aattatgcat tgaagtagtg gacaacgttc aggatgccat 2941 tgaccacatc cacaagtatg gcagctccca cacggatgtc atcgtcacag aggacgaaaa 3001 cacagcggag ttcttcctgc agcacgtaga cagtgcctgt gtgttctgga atgccagcac 3061 tcgcttttct gatggttacc gctttggact gggagctgaa gtgggaatca gtacatcgag 3121 aatccacgcc cggggaccag taggacttga gggactgctt actactaagt ggctgctgcg 3181 agggaaggac cacgtggtct cagatttctc agagcatgga agtttaaaat atcttcatga 3241 gaacctccct attcctcaga gaaacaccaa cgaattcggt ggaggcggat caggaggcgg 3301 tggatctatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg 3361 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg 3421 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc 3481 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt 3541 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg 3601 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct 3661 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg 3721 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga 3781 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta 3841 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta 3901 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca 3961 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaggc 4021 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta 4081 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 4141 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 4201 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 4261 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg 4321 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 4381 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 4441 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 4501 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 4561 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 4621 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 4681 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 4741 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg 4801 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 4861 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 4921 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 4981 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 5041 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 5101 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 5161 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 5221 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 5281 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 5341 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 5401 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 5461 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 5521 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 5581 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 5641 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 5701 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 5761 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 5821 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 5881 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 5941 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac 6001 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg 6061 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc 6121 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa 6181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 6241 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc 6301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 6361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 6421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 6481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 6541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 6601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 6661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 6721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 6781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 6841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 6901 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 6961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 7021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 7081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 7141 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 7201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt 7261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 7381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 7441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 7501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 7561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 7621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 7681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 7741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 7801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 7861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 7921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 7981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 8101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 8161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 8221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 8281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 8341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 8401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 8461 cctgacgtcg acggatcggg agatctcccg atcccctatg gt //