LOCUS       Exported                4748 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pcDNA1-hFADD-V121N, complete sequence.
ACCESSION   LT727123
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4748)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Mammalian expression vector pcDNA1-hFADD-V121N"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4607."
                     /db_xref="taxon:1944921"
     rep_origin      25..160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(36..111)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     rep_origin      complement(262..374)
                     /direction=LEFT
                     /label=polyoma ori
                     /note="polyoma ori"
     polyA_signal    1006..1140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1066..1162)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      1066..1162
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     misc_feature    complement(1163..1561)
                     /label=3' UTR of SV40 small t-antigen
                     /note="3' UTR of SV40 small t-antigen"
     CDS             complement(1412..1432)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1562..1628)
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     intron          1562..1627
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1629..1703)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL87512.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     promoter        1711..1729
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      1711..1727
                     /regulatory_class="promoter"
                     /note="SP6 promoter"
     misc_feature    complement(1871..1909)
                     /label=E-tag
                     /note="E-tag"
     CDS             complement(1919..2542)
                     /codon_start=1
                     /note="unnamed protein product; hFADD mutant"
                     /protein_id="SJL87513.1"
                     /translation="MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDL
                     FSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVI
                     CDNVGKDWRRLARQLKNSDTKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVG
                     ALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSWNSDASTSEAS"
     misc_feature    complement(2180..2185)
                     /label=V121N
                     /note="V121N"
     promoter        complement(2561..2579)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(2563..2579)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     regulatory      complement(2624..3207)
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     promoter        complement(2624..2827)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2828..3207
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    complement(3362..3564)
                     /label=supF
                     /note="supF"
     tRNA            complement(3366..3545)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     rep_origin      3688..4146
                     /label=M13 ori
                     /note="M13 ori"
     rep_origin      complement(4165..4397)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
ORIGIN
        1 gaattagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
       61 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
      121 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagta agacccgcac
      181 ccgttcccaa actgcttatt aaagggggta tggaggtgct ggaccttgtg acagggccag
      241 acagtgtgac agaaatagaa gcagtcctgt tttgacaagt tgcctctgga agcctctaca
      301 atgcctctct tctttttctc cagagtaagc gggcccaggg gtccccggcc tctgcttaat
      361 actaaaaaaa acagctgttg tcatagtaat gattgggtgg aaacattcta ggcctgggtg
      421 gagaggcttt tgcttcctct tgcaaaacca cacgccctct ggagggcgtt gcctagcaac
      481 taattaaaag aggatgtcgc acggccagct gcgtcagtta gtccacttcc tgcttaactg
      541 acttgacatt ttctatttta agagtcggga ggaaaattac tgtgttggag gccctccgcc
      601 atcttctgaa gctgatcaag taagtgaatt ttcaaaatgg gagccgcact gactattcta
      661 gtagacctca tcgagggatt agctgaagtg tctaccctta cgggactctc ggcagaagct
      721 attttatctg gagaagccct cgctgccctt gatggcgaaa ttacagctct gactttggag
      781 ggtgtaatga gttcggagac agccctagca actatgggta tttcagagga ggtgtatggg
      841 tttgtcagta ctgtgcctgt gtttgtaagt cgaacagcgg gggctatatg gctgatgcag
      901 acagttcaag gtgcctctac tatttcccta ggaatacagc ggtacctaca caacgaagag
      961 gtccctactg taaatagaaa tatggcgttg ataccatggc gggatgatcc agacatgata
     1021 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1081 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1141 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     1201 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
     1261 tatacatcaa atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg
     1321 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta
     1381 gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag
     1441 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca
     1501 ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa
     1561 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta
     1621 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga
     1681 agtaaggttc cttcacaaag atcctctagc atttaggtga cactatagaa tagggccctc
     1741 tagagtcgac attccacaga cagccctcat agttagcgta acgatctaaa gttttgtcgt
     1801 ctttccagac gttagtaaat gaattttctg tatgaggttt tgctaaacaa ctttcaacag
     1861 tctatgcggc acgcggttcc agcggatccg gatacggcac cggcgcacct gcggccgcgg
     1921 acgcttcgga ggtagatgcg tctgagttcc atgacatcgg ggacatggcc ccactcctgt
     1981 tctggaggtc acgggcctgc tgaacctctt gtaccaggtc agccaccagg ttcatctggc
     2041 aggacctgag agcccccacc aggtgggcca ctgttgcgtt ctccttctct gtgttcttcc
     2101 agattctcag tgactcccgc acacgctctg tcaggttgcg ggggtatctg tcctcgatgc
     2161 tgtcgatctt ggtgtctgaa ttcttgagct gacgagccag ccttctccaa tctttcccca
     2221 cattatcaca tatgacgtta aatgctgcac acaggtcttc ttccccaggc gcggccccgg
     2281 ccgccgcccc cgcctcgaag tcgtcgacgc gccgcagcag gtcgtggcgc cgcagggagg
     2341 cgagcagctc gcgcaggagc tcggtgtgcc cgggctccag gtcgttctgc tccagcagca
     2401 tggagaagag gtctaggccg ctctgcacgc gctccagctt gcgcttgccc acgcgcccga
     2461 ggcataggaa cttgagctcg gtcagctcgc tgctcgacag gctggacgac accgagtgca
     2521 gcagcaccag gaacgggtcc atggcggaag cttgggtctc cctatagtga gtcgtattaa
     2581 tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga
     2641 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     2701 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     2761 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     2821 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     2881 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     2941 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     3001 agtccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     3061 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     3121 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     3181 attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca
     3241 gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg
     3301 ttgtagctta aattttactc gcgcactact cagcgacctc caacacacaa gcagggagca
     3361 gattctttcg gacttttgaa agtgatggtg gtgggggaag gattcgaacc ttcgaagtcg
     3421 atgacggcag atttagagtc tgctcccttt ggccgctcgg gaaccccacc acgggtaatg
     3481 cttttactgg cctgctccct tatcgggaag cggggcgcat catatcaaat gacgcgccgc
     3541 tgtaaagtgt tacgttgaga aagaccgcgg taatccagct ggcgtaccgt tcctgtctaa
     3601 aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt
     3661 atacggtctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     3721 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3781 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3841 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3901 atttgggtga tggttcacgt agtgggcggc catcgccctg atagacggtt tttcgccctt
     3961 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4021 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4081 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4141 caatttctag ctagaagcta gcttgcgttg ctggcgtttt tccataggct ccgcccccct
     4201 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4261 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4321 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4381 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4441 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4501 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4561 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     4621 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4681 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4741 attacgcc
//
