LOCUS       Exported                4276 bp DNA     circular SYN 14-AUG-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pWB980-ori
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   A
  TITLE     Direct Submission
  JOURNAL   Exported Aug 14, 2024 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             309..1313
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /note="from Enterococcus faecalis plasmid pAM-alpha-1"
                     /db_xref="GI:22652809"
                     /protein_id="AAN03827.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             1473..2243
                     /codon_start=1
                     /product="aminoglycoside O-nucleotidyltransferase 
                     ANT(4')-Ia [Staphylococcus aureus]"
                     /label=Kan
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
                     QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHG
                     QFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTT
                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD
                     SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     rep_origin      complement(2373..2961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3727..3748
                     /label=pwb980-p43-F
     promoter        3772..4063
                     /label=P43
     CDS             4105..4191
                     /codon_start=1
                     /label=sacB signal peptide
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFA"
ORIGIN
        1 agaacctaaa aagaacgaat ttgaactaac tcataaccga gaggtaaaaa aagaacgaag
       61 tcgagatcag ggaatgagtt tataaaataa aaaaagcacc tgaaaaggtg tctttttttg
      121 atggttttga acttgttctt tcttatcttg atacatatag aaataacgtc atttttattt
      181 tagttgctga aaggtgcgtt gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc
      241 ttaaaattgg ttgcacagaa aaaccccatc tgttaaagtt ataagtgact aaacaaataa
      301 ctaaatagat gggggtttct tttaatatta tgtgtcctaa tagtagcatt tattcagatg
      361 aaaaatcaag ggttttagtg gacaagacaa aaagtggaaa agtgagacca tggagagaaa
      421 agaaaatcgc taatgttgat tactttgaac ttctgcatat tcttgaattt aaaaaggctg
      481 aaagagtaaa agattgtgct gaaatattag agtataaaca aaatcgtgaa acaggcgaaa
      541 gaaagttgta tcgagtgtgg ttttgtaaat ccaggctttg tccaatgtgc aactggagga
      601 gagcaatgaa acatggcatt cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc
      661 caacagttcg ttggttgttt ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat
      721 taaataagag tttgtcagat atggctcaag gatttcgccg aatgatgcaa tataaaaaaa
      781 ttaataaaaa tcttgttggt tttatgcgtg caacggaagt gacaataaat aataaagata
      841 attcttataa tcagcacatg catgtattgg tatgtgtgga accaacttat tttaagaata
      901 cagaaaacta cgtgaatcaa aaacaatgga ttcaattttg gaaaaaggca atgaaattag
      961 actatgatcc aaatgtaaaa gttcaaatga ttcgaccgaa aaataaatat aaatcggata
     1021 tacaatcggc aattgacgaa actgcaaaat atcctgtaaa ggatacggat tttatgaccg
     1081 atgatgaaga aaagaatttg aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa
     1141 ggttaatctc ctatggtggt ttgttaaaag aaatacataa aaaattaaac cttgatgaca
     1201 cagaagaagg cgatttgatt catacagatg atgacgaaaa agccgatgaa gatggatttt
     1261 ctattattgc aatgtggaat tgggaacgga aaaattattt tattaaagag tagttcaaca
     1321 aacgggccag tttgttgaag attagatgct ataattgtta ttaaaaggat tgaaggatgc
     1381 ttaggaagac gagttattaa tagctgaata agaacggtgc tctccaaata ttcttattta
     1441 gaaaagcaaa tctaaaatta tctgaaaagg gaatgagaat agtgaatgga ccaataataa
     1501 tgactagaga agaaagaatg aagattgttc atgaaattaa ggaacgaata ttggataaat
     1561 atggggatga tgttaaggct attggtgttt atggctctct tggtcgtcag actgatgggc
     1621 cctattcgga tattgagatg atgtgtgtca tgtcaacaga ggaagcagag ttcagccatg
     1681 aatggacaac cggtgagtgg aaggtggaag tgaattttga tagcgaagag attctactag
     1741 attatgcatc tcaggtggaa tcagattggc cgcttacaca tggtcaattt ttctctattt
     1801 tgccgattta tgattcaggt ggatacttag agaaagtgta tcaaactgct aaatcggtag
     1861 aagcccaaac gttccacgat gcgatttgtg cccttatcgt agaagagctg tttgaatatg
     1921 caggcaaatg gcgtaatatt cgtgtgcaag gaccgacaac atttctacca tccttgactg
     1981 tacaggtagc aatggcaggt gccatgttga ttggtctgca tcatcgcatc tgttatacga
     2041 cgagcgcttc ggtcttaact gaagcagtta agcaatcaga tcttccttca ggttatgacc
     2101 atctgtgcca gttcgtaatg tctggtcaac tttccgactc tgagaaactt ctggaatcgc
     2161 tagagaattt ctggaatggg attcaggagt ggacagaacg acacggatat atagtggatg
     2221 tgtcaaaacg cataccattt tgaagcggta tcagctcact caaaggcggt aatacggtta
     2281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3121 tggtctgaca gaatgattca ccggtttgta caggtgcgga gtcgtttatt gctggtactg
     3181 ctagttgccg cattgaagta gagggaattg atgaattata tcaacatatt aagcctttgg
     3241 gcattttgca ccccaataca tcattaaaag atcagtggtg ggatgaacga gactttgcag
     3301 taattgatcc cgacaacaat ttgattagct tttttcaaca aataaaaagc taaaatctat
     3361 tattaatctg ttcagcaatc gggcgcgatt gctgaataaa agatacgaga gacctctctt
     3421 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     3481 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     3541 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     3601 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     3661 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     3721 ggtcggcact gaattcgagc tcagcattat tgagtggatg attatattcc ttttgatagg
     3781 tggtatgttt tcgcttgaac ttttaaatac agccattgaa catacggttg atttaataac
     3841 tgacaaacat caccctcttg ctaaagcggc caaggacgct gccgccgggg ctgtttgcgt
     3901 ttttgccgtg atttcgtgta tcattggttt acttattttt ttgccaaagc tgtaatggct
     3961 gaaaattctt acatttattt tacattttta gaaatgggcg tgaaaaaaag cgcgcgatta
     4021 tgtaaaatat aaagtgatag cggtaccagg agggctggaa gaagcagacc gctaacacag
     4081 tacataaaaa aggagacatg aacgatgaac atcaaaaagt ttgcaaaaca agcaacagta
     4141 ttaaccttta ctaccgcact gctggcagga ggcgcaactc aagcttttgc ctcgagctcg
     4201 gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gctagcttca gcacaattcc
     4261 aagaaagaca cgattt
//
