LOCUS       62056_22205        1835 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..1835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(80..668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(842..1699)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1700..1804)
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatat cggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
       61 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
      901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      961 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1801 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
