LOCUS       Exported               11739 bp DNA     circular SYN 21-JUL-2025
DEFINITION  Reporter vector pUC18T-mini-Tn7T-lux-Tp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, 
            Goldberg JB.
  TITLE     Construction of conjugatable Mini-Tn7 Vectors for Bioluminescent 
            Detection and Single Copy Promoter lux Reporter Analysis in 
            Gram-negative Bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, 
            Goldberg JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2013) Microbiology, Immunology, and Cancer 
            Biology, University of Virginia, PO Box 800734, Charlottesville, VA 
            22908, USA
REFERENCE   3  (bases 1 to 11739)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11739)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11739)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-MAR-2013) Microbiology, Immunology, and Cancer Biology,
            University of Virginia, PO Box 800734, Charlottesville, VA 22908,
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     349..365
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      393..487
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      590..676
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    722..769
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(834..1067)
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     regulatory      1263..1304
                     /label=promoter 2
                     /note="promoter 2"
                     /regulatory_class="promoter"
     promoter        complement(1393..1421)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1473..1520
                     /label=FRT
                     /note="FRT"
     protein_bind    1473..1520
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1473..1495
                     /label=Plux seq F
                     /note="Plux seq F"
     promoter        1616..1644
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     2041..2063
                     /label=Plux seq R
                     /note="Plux seq R"
     CDS             2491..3930
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             3946..4866
                     /codon_start=1
                     /label=LuxD
                     /note="LuxD acyltransferase"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             4918..5997
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             6015..6995
                     /codon_start=1
                     /label=LuxB
                     /note="LuxB luciferase subunit"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             7177..8286
                     /label=LuxE
                     /note="LuxE"
     mobile_element  complement(9020..9185)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(9344..9452)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(9684..10272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10446..11303)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(11304..11408)
                     /label=AmpR promoter
ORIGIN
        1 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
       61 ctattacgcc agctagagga ccagccgcgt aacctggcaa aatcggttac ggttgagtaa
      121 taaatggatg ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa
      181 tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca
      241 gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca
      301 ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggggttcg aggtcgacgg
      361 tatcgataag ctagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac
      421 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
      481 tgagaatcca agctagactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
      541 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg
      601 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      661 cctgagtagg acaaatccgc cgctaggagc ttgcggcccg gacgatcgaa ttggggatct
      721 tgaagtacct attccgaagt tcctattctc tagaaagtat aggaacttca gagcgctttt
      781 gaagctgatg tgcttaaaaa cttactcaat ggaataatcc tagataattc ttaggccaca
      841 cgttcaagtg cagccacagg ataaatttgc actgagcctg ggtgggattc ggactcgacc
      901 gcatagcctt caggagtgag ttttgtgcaa taccaaccga cgacttgacc ctgccaagcg
      961 gcaccagatt tcttgcgtac gcgatcccct aagccaaagg tggcactcag gggaagcgca
     1021 aactgccctg caacgggagc gttggcttca tcgctacttt gacccatgtc gaatccttct
     1081 tgtgaatcta ttatggcgac aagcaaatcg agctctgact gcctacccca caacaactat
     1141 cagaaagcac cagcacaacg gctgcctaac tttgttttag ggcgactgcc ctgctgcgta
     1201 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     1261 gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
     1321 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     1381 tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg tgaattatcc
     1441 attgctgttg acaaagggaa tcaggggatc ttgaagttcc tattccgaag ttcctattct
     1501 ctagaaagta taggaacttc agagcgcttt tgaagctaat tcgatcatgc atgagctcac
     1561 tagtggatcc actagtaacg gccgccagtg tgctggaatt cggcttcgaa cccagttgac
     1621 ataagcctgt tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc
     1681 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1741 tgactgtttt tttgtacagt ctagcctcgg gcatccaagc tagctaagcg cgttacgccg
     1801 tgggtcgatg tttgatgtta tggaacagca acgatgttac gcagcagggt agtcgcccta
     1861 aaacaaagtt aggcagccgt tgtgctggtg ctttctagta gttgttgtgg ggtaggcagt
     1921 cagagttcga tttgcttgtc gccataatag attcacaaga agcatatgac atgggtcaag
     1981 ccgaattctg cagatatcca tcacactggc ggccgctcga gaagctgatc cccaattctt
     2041 ctttagaaat ctgccggtaa aaattagatt gctattcaat ctatttctat cggtatttgt
     2101 gaaataatac tcaggataat aatttacata aatattatca cgcattagag aagagcatga
     2161 cttttttaat ttaaactttt cattaacaaa tcttgttgat atgaaaattt tcctttgcta
     2221 ttttaacaga tattaaaacg ggaataggcg ttatattgac gatccattca gttagattaa
     2281 aaaccttgag cagaaaattt atattattat cataattatg acgaaagtta caggccagga
     2341 accacgtagt cagaatctga ttttctatat atttgttatt tacatcgtca taacacaaaa
     2401 atataagaag caagtgttgg tacgaccagt tcgcaagata gttaaacagc aacttaagtt
     2461 gaaattaccc ccattaaatg gatggcaaat atgactaaaa aaatttcatt cattattaac
     2521 ggccaggttg aaatctttcc cgaaagtgat gatttagtgc aatccattaa ttttggtgat
     2581 aatagtgttt acctgccaat attgaatgac tctcatgtaa aaaacattat tgattgtaat
     2641 ggaaataacg aattacggtt gcataacatt gtcaattttc tctatacggt agggcaaaga
     2701 tggaaaaatg aagaatactc aagacgcagg acatacattc gtgacttaaa aaaatatatg
     2761 ggatattcag aagaaatggc taagctagag gccaattgga tatctatgat tttatgttct
     2821 aaaggcggcc tttatgatgt tgtagaaaat gaacttggtt ctcgccatat catggatgaa
     2881 tggctacctc aggatgaaag ttatgttcgg gcttttccga aaggtaaatc tgtacatctg
     2941 ttggcaggta atgttccatt atctgggatc atgtctatat tacgcgcaat tttaactaag
     3001 aatcagtgta ttataaaaac atcgtcaacc gatcctttta ccgctaatgc attagcgtta
     3061 agttttattg atgtagaccc taatcatccg ataacgcgct ctttatctgt tatatattgg
     3121 ccccaccaag gtgatacatc actcgcaaaa gaaattatgc gacatgcgga tgttattgtc
     3181 gcttggggag ggccagatgc gattaattgg gcggtagagc acgcgccatc ttatgctgat
     3241 gtgattaaat ttggttctaa aaagagtctt tgcattatcg ataatcctgt tgatttgacg
     3301 tccgcagcga caggtgcggc tcatgatgtt tgtttttacg atcagcgagc ttgtttttct
     3361 gcccaaaaca tatattacat gggaaatcat tatgaggaat ttaagttagc gttgatagaa
     3421 aaacttaatc tatatgcgca tatattaccg aatgccaaaa aagattttga tgaaaaggcg
     3481 gcctattctt tagttcaaaa agaaagcctg tttgctggat taaaagtaga ggtggatatt
     3541 catcaacgtt ggatgattat tgagtcaaat gcaggtgtgg aatttaatca accacttggc
     3601 agatgtgtgt accttcatca cgtcgataat attgagcaaa tattgcctta tgttcaaaaa
     3661 aataagacgc aaaccatatc tatttttcct tgggagtcat catttaaata tcgagatgcg
     3721 ttagcattaa aaggtgcgga aaggattgta gaagcaggaa tgaataacat atttcgagtt
     3781 ggtggatctc atgacggaat gagaccgttg caacgattag tgacatatat ttctcatgaa
     3841 aggccatcta actatacggc taaggatgtt gcggttgaaa tagaacagac tcgattcctg
     3901 gaagaagata agttccttgt atttgtccca taataggtaa aaagtatgga aaatgaatca
     3961 aaatataaaa ccatcgacca cgttatttgt gttgaaggaa ataaaaaaat tcatgtttgg
     4021 gaaacgctgc cagaagaaaa cagcccaaag agaaagaatg ccattattat tgcgtctggt
     4081 tttgcccgca ggatggatca ttttgctggt ctggcggaat atttatcgcg gaatggattt
     4141 catgtgatcc gctatgattc gcttcaccat gttggcttga gttcagggac aattgatgaa
     4201 tttacaatgt ctataggaaa gcagagcttg ttagcagtgg ttgattggtt aactacacga
     4261 aaaataaata acttcggtat gttggcttcc agcttatctg cgcggatagc ttatgcaagc
     4321 ctatctgaaa tcaatgcttc gtttttaatc accgcagtcg gtgttgttaa cttaagatat
     4381 tctcttgaaa gagctttagg gtttgattat ctcagtctac ccattaatga attgccggat
     4441 aatctggatt ttgaaggcca taaattgggt gctgaagtct ttgcgagaga ttgtcttgat
     4501 tttggttggg aagatttagc ttctacaatt aataacatga tgtatcttga tataccgttt
     4561 attgctttta ctgcaaataa cgataattgg gtcaagcaag atgaagttat cacattgtta
     4621 tcaaatattc gtagtaatcg atgcaagata tattctttgt taggaagttc gcatgacttg
     4681 agtgaaaatt tagtggtcct gcgcaatttt tatcaatcgg ttacgaaagc cgctatcgcg
     4741 atggataatg atcatctgga tattgatgtt gatattactg aaccgtcatt tgaacattta
     4801 actattgcga cagtcaatga acgccgaatg agaattgaga ttgaaaatca agcaatttct
     4861 ctgtcttaaa atctattgag atattctatc actcaaatag caatataagg actctctatg
     4921 aaatttggaa actttttgct tacataccaa cctccccaat tttctcaaac agaggtaatg
     4981 aaacgtttgg ttaaattagg tcgcatctct gaggagtgtg gttttgatac cgtatggtta
     5041 ctggagcatc atttcacgga gtttggtttg cttggtaacc cttatgtcgc tgctgcatat
     5101 ttacttggcg cgactaaaaa attgaatgta ggaactgccg ctattgttct tcccacagcc
     5161 catccagtac gccaacttga agatgtgaat ttattggatc aaatgtcaaa aggacgattt
     5221 cggtttggta tttgccgagg gctttacaac aaggactttc gcgtattcgg cacagatatg
     5281 aataacagtc gcgccttagc ggaatgctgg tacgggctga taaagaatgg catgacagag
     5341 ggatatatgg aagctgataa tgaacatatc aagttccata aggtaaaagt aaaccccgcg
     5401 gcgtatagca gaggtggcgc accggtttat gtggtggctg aatcagcttc gacgactgag
     5461 tgggctgctc aatttggcct accgatgata ttaagttgga ttataaatac taacgaaaag
     5521 aaagcacaac ttgagcttta taatgaagtg gctcaagaat atgggcacga tattcataat
     5581 atcgaccatt gcttatcata tataacatct gtagatcatg actcaattaa agcgaaagag
     5641 atttgccgga aatttctggg gcattggtat gattcttatg tgaatgctac gactattttt
     5701 gatgattcag accaaacaag aggttatgat ttcaataaag ggcagtggcg tgactttgta
     5761 ttaaaaggac ataaagatac taatcgccgt attgattaca gttacgaaat caatcccgtg
     5821 ggaacgccgc aggaatgtat tgacataatt caaaaagaca ttgatgctac aggaatatca
     5881 aatatttgtt gtggatttga agctaatgga acagtagacg aaattattgc ttccatgaag
     5941 ctcttccagt ctgatgtcat gccatttctt aaagaaaaac aacgttcgct attatattag
     6001 ctaaggagaa agaaatgaaa tttggattgt tcttccttaa cttcatcaat tcaacaactg
     6061 ttcaagaaca aagtatagtt cgtatgcagg aaataacgga gtatgttgat aagttgaatt
     6121 ttgaacagat tttagtgtat gaaaatcatt tttcagataa tggtgttgtc ggcgctcctc
     6181 tgactgtttc tggttttctg ctcggtttaa cagagaaaat taaaattggt tcattaaatc
     6241 acatcattac aactcatcat cctgtccgca tagcggagga agcgtgctta ttggatcagt
     6301 taagtgaagg gagatttatt ttagggttta gtgattgcga aaaaaaagat gaaatgcatt
     6361 tttttaatcg cccggttgaa tatcaacagc aactatttga agagtgttat gaaatcatta
     6421 acgatgcttt aacaacaggc tattgtaatc cagataacga tttttatagc ttccctaaaa
     6481 tatctgtaaa tccccatgct tatacgccag gcggacctcg gaaatatgta acagcaacca
     6541 gtcatcatat tgttgagtgg gcggccaaaa aaggtattcc tctcatcttt aagtgggatg
     6601 attctaatga tgttagatat gaatatgctg aaagatataa agccgttgcg gataaatatg
     6661 acgttgacct atcagagata gaccatcagt taatgatatt agttaactat aacgaagata
     6721 gtaataaagc taaacaagag acgcgtgcat ttattagtga ttatgttctt gaaatgcacc
     6781 ctaatgaaaa tttcgaaaat aaacttgaag aaataattgc agaaaacgct gtcggaaatt
     6841 atacggagtg tataactgcg gctaagttgg caattgaaaa gtgtggtgcg aaaagtgtat
     6901 tgctgtcctt tgaaccaatg aatgatttga tgagccaaaa aaatgtaatc aatattgttg
     6961 atgataatat taagaagtac cacatggaat atacctaata gatttcgagt tgcagcgagg
     7021 cggcaagtga acgaatcccc aggagcatag ataactatgt gactggggtg agtgaaagca
     7081 gccaacaaag cagcagcttg aaagatgaag ggtataaaag agtatgacag cagtgctgcc
     7141 atactttcta atattatctt gaggagtaaa acaggtatga cttcatatgt tgataaacaa
     7201 gaaattacag caagctcaga aattgatgat ttgatttttt cgagcgatcc attagtgtgg
     7261 tcttacgacg agcaggaaaa aatcagaaag aaacttgtgc ttgatgcatt tcgtaatcat
     7321 tataaacatt gtcgagaata tcgtcactac tgtcaggcac acaaagtaga tgacaatatt
     7381 acggaaattg atgacatacc tgtattccca acatcggttt ttaagtttac tcgcttatta
     7441 acttctcagg aaaacgagat tgaaagttgg tttaccagta gcggcacgaa tggtttaaaa
     7501 agtcaggtgg cgcgtgacag attaagtatt gagagactct taggctctgt gagttatggc
     7561 atgaaatatg ttggtagttg gtttgatcat caaatagaat tagtcaattt gggaccagat
     7621 agatttaatg ctcataatat ttggtttaaa tatgttatga gtttggtgga attgttatat
     7681 cctacgacat ttaccgtaac agaagaacga atagattttg ttaaaacatt gaatagtctt
     7741 gaacgaataa aaaatcaagg gaaagatctt tgtcttattg gttcgccata ctttatttat
     7801 ttactctgcc attatatgaa agataaaaaa atctcatttt ctggagataa aagcctttat
     7861 atcataaccg gaggcggctg gaaaagttac gaaaaagaat ctctgaaacg tgatgatttc
     7921 aatcatcttt tatttgatac tttcaatctc agtgatatta gtcagatccg agatatattt
     7981 aatcaagttg aactcaacac ttgtttcttt gaggatgaaa tgcagcgtaa acatgttccg
     8041 ccgtgggtat atgcgcgagc gcttgatcct gaaacgttga aacctgtacc tgatggaacg
     8101 ccggggttga tgagttatat ggatgcgtca gcaaccagtt atccagcatt tattgttacc
     8161 gatgatgtcg ggataattag cagagaatat ggtaagtatc ccggcgtgct cgttgaaatt
     8221 ttacgtcgcg tcaatacgag gacgcagaaa gggtgtgctt tgagcttaac cgaagcgttt
     8281 gatagttgat atcctttgcc taattgtaag tggaatgctt gcgttatata aatctgaatg
     8341 acatctacac tttacaaaat tctccaaaac atccacattt gggtacttga tagaggttta
     8401 tggggttggt ttaacattgt tctcattgtt attattggct caaagcaaaa ggagataaca
     8461 tgaaaaaatt ggcagttatg cttgcattgg gaatgattag ctttggtgca atggcagttg
     8521 atgggtataa agatgcaaag tttggcatga cagaagaaga gtttctttcg aagaggttat
     8581 gtgattttga aaaatttgag ggagattctc gaatagaaga agtatcactt tattcatgtt
     8641 ctgacttttc gtttgctaac aaaaagcgtg aagcaatggc atttttttta aatgggaaat
     8701 ttaaaagatt agagattaat attggcagac ttgtgaagcc agtaagcaaa tcgttaacga
     8761 aaaagtacgg agatggatca tcgtatccat caaaagaaga atttgagaac gcgctaaaat
     8821 acaatggaac tatgtctata ggttatgata ataatacggt attagttgat atacatataa
     8881 tatgtggcaa agaaggcata gaaaccagtc aactgattta tacgagtcca gatgtttata
     8941 cgctcccaga tttcggagaa aaaatccagg aattaaaggg attaaaggaa ttgggtacct
     9001 cgcgaaggcc ttgcaggcca accagataag tgaaatctag ttccaaacta ttttgtcatt
     9061 tttaattttc gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc
     9121 cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc
     9181 ccacagcggg gcatttttct tcctgttatg tttgggcgct gcattaatga atcggccaac
     9241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgacccgc
     9301 tgcgctcggt cgttcggctg cggcgagcgg tatcagagct tatcggccag cctcgcagag
     9361 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc
     9421 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa
     9481 agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg
     9541 aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaagtata ccttaaggaa
     9601 tcccctgata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9721 aatcgacgct cgagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10081 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    10141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    10441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    10501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    10561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    10621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    10681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    10741 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    11101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    11221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    11281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    11341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    11401 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    11461 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    11521 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    11581 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    11641 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    11701 ataccgcaca gatgcgtaag gagaaaatac cgcatcagg
//
