LOCUS       V014802                17509 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014802
VERSION     V014802
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 17509)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..17509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..37
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             174..1223
                     /gene="aroG"
                     /label="Phospho-2-dehydro-3-deoxyheptonate aldolase,
                     Phe-sensitive"
                     /note="Phospho-2-dehydro-3-deoxyheptonate aldolase,
                     Phe-sensitive from Escherichia coli (strain K12).
                     Accession#: P0AB91"
     CDS             1227..2294
                     /gene="aroF"
                     /label="Phospho-2-dehydro-3-deoxyheptonate aldolase,
                     Tyr-sensitive"
                     /note="Phospho-2-dehydro-3-deoxyheptonate aldolase,
                     Tyr-sensitive from Escherichia coli (strain K12).
                     Accession#: P00888"
     CDS             2298..4286
                     /gene="tktA"
                     /label="Transketolase 1"
                     /note="Transketolase 1 from Escherichia coli (strain K12).
                     Accession#: P27302"
     CDS             4704..5933
                     /gene="serA"
                     /label="D-3-phosphoglycerate dehydrogenase"
                     /note="D-3-phosphoglycerate dehydrogenase from Escherichia
                     coli (strain K12). Accession#: P0A9T0"
     promoter        6040..6068
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             6116..7303
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             7945..8133
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    8238..8378
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8564..9152)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        9299..9376
                     /label="lacI promoter"
     promoter        10455..10483
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    10491..10507
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10515..10531
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             10613..12172
                     /gene="trpE"
                     /label="Anthranilate synthase component 1"
                     /note="Anthranilate synthase component 1 from Escherichia
                     coli (strain K12). Accession#: P00895"
     CDS             12175..13767
                     /gene="trpGD"
                     /label="Bifunctional protein TrpGD"
                     /note="Bifunctional protein TrpGD from Escherichia coli
                     (strain K12). Accession#: P00904"
     CDS             13771..15129
                     /gene="trpC"
                     /label="Tryptophan biosynthesis protein TrpCF"
                     /note="Tryptophan biosynthesis protein TrpCF from
                     Escherichia coli (strain K12). Accession#: P00909"
     CDS             15144..16334
                     /gene="trpB"
                     /label="Tryptophan synthase beta chain"
                     /note="Tryptophan synthase beta chain from Shigella
                     flexneri. Accession#: P0A880"
     CDS             16337..17140
                     /gene="trpA"
                     /label="Tryptophan synthase alpha chain"
                     /note="Tryptophan synthase alpha chain from Escherichia
                     coli (strain K12). Accession#: P0A877"
ORIGIN
        1 gaattctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga gcggataaca
       61 atttcacaca ggaaacagct atgaccatga ttacggattc actggaattc cttgttgatc
      121 gcgctttgat atacgccgag atctaagata agtatggcaa cactggaaca gacatgaatt
      181 atcagaacga cgatttacgc atcaaagaaa tcaaagagtt acttcctcct gtcgcattgc
      241 tggaaaaatt ccccgctact gaaaatgccg cgaatacggt tgcccatgcc cgaaaagcga
      301 tccataagat cctgaaaggt aatgatgatc gcctgttggt tgtgattggc ccatgctcaa
      361 ttcatgatcc tgtcgcggca aaagagtatg ccactcgctt gctggcgctg cgtgaagagc
      421 tgaaagatga gctggaaatc gtaatgcgcg tctattttga aaagccgcgt accacggtgg
      481 gctggaaagg gctgattaac gatccgcata tggataatag cttccagatc aacgacggtc
      541 tgcgtatagc ccgtaaattg ctgcttgata ttaacgacag cggtctgcca gcggcaggtg
      601 agtttctcaa tatgatcacc ccacaatatc tcgctgacct gatgagctgg ggcgcaattg
      661 gcgcacgtac caccgaatcg caggtgcacc gcgaactggc atcagggctt tcttgtccgg
      721 tcggcttcaa aaatggcacc gacggtacga ttaaagtggc tatcgatgcc attaatgccg
      781 ccggtgcgcc gcactgcttc ctgttcgtaa cgaaatgggg gcattcggcg attgtgaata
      841 ccagcggtaa cggcgattgc catatcattc tgcgcggcgg taaagagcct aactacagcg
      901 cgaagcacgt tgctgaagtg aaagaagggc tgaacaaagc aggcctgcca gcacaggtga
      961 tgatcgattt cagccatgct aactcgtcca aacaattcaa aaagcagatg gatgtttgtg
     1021 ctgacgtttg ccagcagatt gccggtggcg aaaaggccat tattggcgtg atggtggaaa
     1081 gccatctggt ggaaggcaat cagagcctcg agagcgggga gccgctggcc tacggtaaga
     1141 gcatcaccga tgcctgcatc ggctgggaag ataccgatgc tctgttacgt caactggcga
     1201 atgcagtaaa agcgcgtcgc gggtaaatgc aaaaagacgc gctgaataac gtacatatta
     1261 ccgacgaaca ggttttaatg actccggaac aactgaaggc cgcttttcca ttgagcctgc
     1321 aacaagaagc ccagattgct gactcgcgta aaagcatttc agatattatc gccgggcgcg
     1381 atcctcgtct gctggtagta tgtggtcctt gttccattca tgatccggaa actgctctgg
     1441 aatatgctcg tcgatttaaa gcccttgccg cagaggtcag cgatagcctc tatctggtaa
     1501 tgcgcgtcta ttttgaaaaa ccccgtacca ctgtcggctg gaaagggtta attaacgatc
     1561 cccatatgga tggctctttt gatgtagaag ccgggctgca gatcgcgcgt aaattgctgc
     1621 ttgagctggt gaatatggga ctgccactgg cgacggaagc gttagatctg aatagcccgc
     1681 aatacctggg cgatctgttt agctggtcag caattggtgc tcgtacaacg gaatcgcaaa
     1741 ctcaccgtga aatggcctcc gggctttcca tgccggttgg ttttaaaaac ggcaccgacg
     1801 gcagtctggc aacagcaatt aacgctatgc gcgccgccgc ccagccgcac cgttttgttg
     1861 gcattaacca ggcagggcag gttgcgttgc tacaaactca ggggaatccg gacggccatg
     1921 tgatcctgcg cggtggtaaa gcgccgaact atagccctgc ggatgttgcg caatgtgaaa
     1981 aagagatgga acaggcggga ctgcgcccgt ctctgatggt agattgcagc cacggtaatt
     2041 ccaataaaga ttatcgccgt cagcctgcgg tggcagaatc cgtggttgct caaatcaaag
     2101 atggcaatcg ctcaattatt ggtctgatga tcgaaagtaa tatccacgag ggcaatcagt
     2161 cttccgagca accgcgcagt gaaatgaaat acggtgtatc cgtaaccgat gcctgcatta
     2221 gctgggaaat gaccgatgcc ttgctgcgtg aaattcatca ggatctgaac gggcagctga
     2281 cggctcgcgt ggcttaaatg tcctcacgta aagagcttgc caatgctatt cgtgcgctga
     2341 gcatggacgc agtacagaaa gccaaatccg gtcacccggg tgcccctatg ggtatggctg
     2401 acattgccga agtcctgtgg cgtgatttcc tgaaacacaa cccgcagaat ccgtcctggg
     2461 ctgaccgtga ccgcttcgtg ctgtccaacg gccacggctc catgctgatc tacagcctgc
     2521 tgcacctcac cggttacgat ctgccgatgg aagaactgaa aaacttccgt cagctgcact
     2581 ctaaaactcc gggtcacccg gaagtgggtt acaccgctgg tgtggaaacc accaccggtc
     2641 cgctgggtca gggtattgcc aacgcagtcg gtatggcgat tgcagaaaaa acgctggcgg
     2701 cgcagtttaa ccgtccgggc cacgacattg tcgaccacta cacctacgcc ttcatgggcg
     2761 acggctgcat gatggaaggc atctcccacg aagtttgctc tctggcgggt acgctgaagc
     2821 tgggtaaact gattgcattc tacgatgaca acggtatttc tatcgatggt cacgttgaag
     2881 gctggttcac cgacgacacc gcaatgcgtt tcgaagctta cggctggcac gttattcgcg
     2941 acatcgacgg tcatgacgcg gcatctatca aacgcgcagt agaagaagcg cgcgcagtga
     3001 ctgacaaacc ttccctgctg atgtgcaaaa ccatcatcgg tttcggttcc ccgaacaaag
     3061 ccggtaccca cgactcccac ggtgcgccgc tgggcgacgc tgaaattgcc ctgacccgcg
     3121 aacaactggg ctggaaatat gcgccgttcg aaatcccgtc tgaaatctat gctcagtggg
     3181 atgcgaaaga agcaggccag gcgaaagaat ccgcatggaa cgagaaattc gctgcttacg
     3241 cgaaagctta tccgcaggaa gccgctgaat ttacccgccg tatgaaaggc gaaatgccgt
     3301 ctgacttcga cgctaaagcg aaagagttca tcgctaaact gcaggctaat ccggcgaaaa
     3361 tcgccagccg taaagcgtct cagaatgcta tcgaagcgtt cggtccgctg ttgccggaat
     3421 tcctcggcgg ttctgctgac ctggcgccgt ctaacctgac cctgtggtct ggttctaaag
     3481 caatcaacga agatgctgcg ggtaactaca tccactacgg tgttcgcgag ttcggtatga
     3541 ccgcgattgc taacggtatc tccctgcacg gtggcttcct gccgtacacc tccaccttcc
     3601 tgatgttcgt ggaatacgca cgtaacgccg tacgtatggc tgcgctgatg aaacagcgtc
     3661 aggtgatggt ttacacccac gactccatcg gtctgggcga agacggcccg actcaccagc
     3721 cggttgagca ggtcgcttct ctgcgcgtaa ccccgaacat gtctacatgg cgtccgtgtg
     3781 accaggttga atccgcggtc gcgtggaaat acggtgttga gcgtcaggac ggcccgaccg
     3841 cactgatcct ctcccgtcag aacctggcgc agcaggaacg aactgaagag caactggcaa
     3901 acatcgcgcg cggtggttat gtgctgaaag actgcgccgg tcagccggaa ctgattttca
     3961 tcgctaccgg ttcagaagtt gaactggctg ttgctgccta cgaaaaactg actgccgaag
     4021 gcgtgaaagc gcgcgtggtg tccatgccgt ctaccgacgc atttgacaag caggatgctg
     4081 cttaccgtga atccgtactg ccgaaagcgg ttactgcacg cgttgctgta gaagcgggta
     4141 ttgctgacta ctggtacaag tatgttggcc tgaacggtgc tatcgtcggt atgaccacct
     4201 tcggtgaatc tgctccggca gagctgctgt ttgaagagtt cggcttcact gttgataacg
     4261 ttgttgcgaa agcaaaagaa ctgctgtaaa ctagtccatg gggtttaatt gtcggatgcg
     4321 ccgtcagagt ggcgtatccg atgaatcacc acaggcctga taagacgcgc agcgtcgcat
     4381 caggcaatgt gctccattgt tagcaacaaa aaagccgact cacttgcagt cggctttctc
     4441 attttaaacg aatgacgttt acttcgcttt accctggttt gcaaccgccg ctgctttcgc
     4501 tgcgatctcg tcagcattac ccagataata gcgtttcagc ggtttgaaat tctcgtcgaa
     4561 ctcatacacc agcggcacgc cagtcgggat attaagctca agaatccgac gcaaacgttc
     4621 atattgccgc aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca
     4681 tttcaaaaga caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt
     4741 ttctgctggt agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gctggttaca
     4801 ccaacatcga atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg
     4861 atgcccactt catcggcctg cgatcccgta cccatctgac tgaagacgtg atcaacgccg
     4921 cagaaaaact ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg
     4981 cggcggcaaa gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg
     5041 cggagctggt gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta
     5101 aagcgcaccg tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa
     5161 agctgggtat catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc
     5221 tgggaatgta tgtttacttt tatgatattg aaaataaact gccgctgggc aacgccactc
     5281 aggtacagca tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag
     5341 agaatccgtc caccaaaaat atgatgggcg cgaaagaaat ttcactaatg aagcccggct
     5401 cgctgctgat taatgcttcg cgcggtactg tggtggatat tccggcgctg tgtgatgcgc
     5461 tggcgagcaa acatctggcg ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca
     5521 atagcgatcc atttacctct ccgctgtgtg aattcgacaa cgtccttctg acgccacaca
     5581 ttggcggttc gactcaggaa gcgcaggaga atatcggcct ggaagttgcg ggtaaattga
     5641 tcaagtattc tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc
     5701 cactgcacgg tgggcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg
     5761 cgctgaacaa aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat ctgcaaactt
     5821 ccgcccagat gggttatgtg gttattgata ttgaagccga cgaagacgtt gccgaaaaag
     5881 cgctgcaggc aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaattcc
     5941 ccttctctga aaatcaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac
     6001 accgttgccg cgttgctgct ctagagtcaa ttctcatgtt tgacagctta tcatcgataa
     6061 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa
     6121 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     6181 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     6241 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     6301 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     6361 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg
     6421 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     6481 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     6541 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     6601 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     6661 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     6721 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     6781 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     6841 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     6901 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     6961 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     7021 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     7081 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     7141 tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc
     7201 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
     7261 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg
     7321 cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact
     7381 gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc
     7441 cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc
     7501 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
     7561 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
     7621 acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc
     7681 tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct
     7741 acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc
     7801 atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca
     7861 gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga
     7921 aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc
     7981 cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat
     8041 gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc
     8101 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     8161 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     8221 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     8281 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     8341 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     8401 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8461 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8521 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8581 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8641 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8701 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8761 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8821 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8881 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8941 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9001 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9061 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9121 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9181 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9241 ggatcttcac ctagatcctt ttccggaatt aattcgagct cggtacccca tttacgttga
     9301 caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
     9361 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
     9421 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
     9481 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
     9541 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
     9601 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
     9661 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
     9721 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
     9781 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
     9841 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
     9901 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
     9961 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
    10021 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
    10081 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
    10141 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
    10201 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
    10261 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
    10321 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcga taagctctaa
    10381 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
    10441 cttcaagaat tctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg
    10501 ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg gaattccttg
    10561 ttgatcgcgc tttgatatac gccgagatca tgaacaaaat tagagaataa caatgcaaac
    10621 acaaaaaccg actctcgaac tactaacctg cgaaggcgct tatcgcgaca acccgaccgc
    10681 gctttttcac cagttgtgtg gggatcgtcc ggcaacgctg ctgctggaat ccgcagatat
    10741 cgacagcaaa gatgatttaa aaagcctgct actggtagac agtgcgctgc gcattacagc
    10801 attaggtgac actgtcacaa ttaaggcgtt atccggcaat ggtgaagccc tgctggcact
    10861 actggataac gccctgcctg cgggtgtgga aaatgaacaa tcaccaaact gccgtgtgct
    10921 gcgcttcccc cctgtcagtc cactgctgga tgaagacgct cgcttatgct ccctttcggt
    10981 ttttgacgct ttccgtttat tgcagaatct gttgaatgta ccgaaggaag aacgagaagc
    11041 catgttcttc ggcggcctgt tctcttatga ccttgtggcg ggatttgaag atttaccgca
    11101 actgtcagcg gaaaataact gccctgattt ctgtttttat ctcgctgaaa cgctgatggt
    11161 gattgaccat cagaaaaaaa gcacccgtat tcaggccagc ctgtttgctc cgaatgaaga
    11221 agaaaaacaa cgtctcactg ctcgcctgaa cgaactacgt cagcaactga ccgaagccgc
    11281 gccgccgctg ccagtggttt ccgtgccgca tatgcgttgt gaatgtaatc agagcgatga
    11341 agagttcggt ggcgtagtgc gtttgttgca aaaagcgatt cgcgctggag aaattttcca
    11401 ggtggtgcca tctcgccgtt tctctctgcc ctgcccgtca ccgctggcgg cctattacgt
    11461 gctgaaaaag agtaatccca gcccgtacat gttttttatg caggataatg atttcaccct
    11521 atttggcgcg tcgccggaaa gctcgctcaa gtatgatgcc accagccgcc agattgagat
    11581 ctacccgatt gccggaacac gcccacgcgg tcgtcgcgcc gatggttcac tggacagaga
    11641 tctcgacagc cgtattgaac tggaaatgcg taccgatcat aaagagctgt ctgaacatct
    11701 gatgctggtt gatctcgccc gtaatgatct ggcacgcatt tgcacccccg gcagccgcta
    11761 cgtcgccgat ctcaccaaag ttgaccgtta ttcctatgtg atgcacctcg tctctcgcgt
    11821 agtcggcgaa ctgcgtcacg atcttgacgc cctgcacgct tatcgcgcct gtatgaatat
    11881 ggggacgtta agcggtgcgc cgaaagtacg cgctatgcag ttaattgccg aggcggaagg
    11941 tcgtcgccgc ggcagctacg gcggcgcggt aggttatttc accgcgcatg gcgatctcga
    12001 cacctacatt gtgatccgct cggcgctggt ggaaaacggt atcgccaccg tgcaagcggg
    12061 tgctggtgta gtccttgatt ctgttccgca gtcggaagcc gacgaaaccc gtaacaaagc
    12121 ccgcgctgta ctgcgcgcta ttgccaccgc gcatcatgca caggagactt tctgatggct
    12181 gacattctgc tgctcgataa tatcgactct tttacgtaca acctggcaga tcagttgcgc
    12241 agcaatgggc ataacgtggt gatttaccgc aaccatattc cggcgcaaac cttaattgaa
    12301 cgcctggcga ccatgagcaa tccggtgctg atgctttctc ctggccccgg tgtgccgagc
    12361 gaagccggtt gtatgccgga actcctcacc cgcttgcgtg gcaagctgcc cattattggc
    12421 atttgcctcg gacatcaggc gattgtcgaa gcttacgggg gctatgtcgg tcaggcgggc
    12481 gaaattctcc acggtaaagc ctccagcatt gaacatgacg gtcaggcgat gtttgccgga
    12541 ttaacaaacc cgctgccggt ggcgcgttat cactcgctgg ttggcagtaa cattccggcc
    12601 ggtttaacca tcaacgccca ttttaatggc atggtgatgg cagtacgtca cgatgcggat
    12661 cgcgtttgtg gattccagtt ccatccggaa tccattctca ccacccaggg cgctcgcctg
    12721 ctggaacaaa cgctggcctg ggcgcagcag aaactagagc cagccaacac gctgcaaccg
    12781 attctggaaa aactgtatca ggcgcagacg cttagccaac aagaaagcca ccagctgttt
    12841 tcagcggtgg tgcgtggcga gctgaagccg gaacaactgg cggcggcgct ggtgagcatg
    12901 aaaattcgcg gtgagcaccc gaacgagatc gccggggcag caaccgcgct actggaaaac
    12961 gcagcgccgt tcccgcgccc ggattatctg tttgctgata tcgtcggtac tggcggtgac
    13021 ggcagcaaca gtatcaatat ttctaccgcc agtgcgtttg tcgccgcggc ctgtgggctg
    13081 aaagtggcga aacacggcaa ccgtagcgtc tccagtaaat ctggttcgtc cgatctgctg
    13141 gcggcgttcg gtattaatct tgatatgaac gccgataaat cgcgccaggc gctggatgag
    13201 ttaggtgtat gtttcctctt tgcgccgaag tatcacaccg gattccgcca cgcgatgccg
    13261 gttcgccagc aactgaaaac ccgcaccctg ttcaatgtgc tggggccatt gattaacccg
    13321 gcgcatccgc cgctggcgtt aattggtgtt tatagtccgg aactggtgct gccgattgcc
    13381 gaaaccttgc gcgtgctggg gtatcaacgc gcggcggtgg tgcacagcgg cgggatggat
    13441 gaagtttcat tacacgcgcc gacaatcgtt gccgaactgc atgacggcga aattaaaagc
    13501 tatcagctca ccgcagaaga ctttggcctg acaccctacc accaggagca actggcaggc
    13561 ggaacaccgg aagaaaaccg tgacatttta acacgtttgt tacaaggtaa aggcgacgcc
    13621 gcccatgaag cagccgtcgc tgcgaacgtc gccatgttaa tgcgcctgca tggccatgaa
    13681 gatctgcaag ccaatgcgca aaccgttctt gaggtactgc gcagtggttc cgcttacgac
    13741 agagtcaccg cactggcggc acgagggtaa atgatgcaaa ccgttttagc gaaaatcgtc
    13801 gcagacaagg cgatttgggt agaagcccgc aaacagcagc aaccgctggc cagttttcag
    13861 aatgaggttc agccgagcac gcgacatttt tatgatgcgc tacagggtgc gcgcacggcg
    13921 tttattctgg agtgcaagaa agcgtcgccg tcaaaaggcg tgatccgtga tgatttcgat
    13981 ccagcacgca ttgccgccat ttataaacat tacgcttcgg caatttcggt gctgactgat
    14041 gagaaatatt ttcaggggag ctttaatttc ctccccatcg tcagccaaat cgccccgcag
    14101 ccgattttat gtaaagactt cattatcgac ccttaccaga tctatctggc gcgctattac
    14161 caggccgatg cctgcttatt aatgctttca gtactggatg acgaccaata tcgccagctt
    14221 gccgccgtcg ctcacagtct ggagatgggg gtgctgaccg aagtcagtaa tgaagaggaa
    14281 caggagcgcg ccattgcatt gggagcaaag gtcgttggca tcaacaaccg cgatctgcgt
    14341 gatttgtcga ttgatctcaa ccgtacccgc gagcttgcgc cgaaactggg gcacaacgtg
    14401 acggtaatca gcgaatccgg catcaatact tacgctcagg tgcgcgagtt aagccacttc
    14461 gctaacggtt ttctgattgg ttcggcgttg atggcccatg acgatttgca cgccgccgtg
    14521 cgccgggtgt tgctgggtga gaataaagta tgtggcctga cgcgtgggca agatgctaaa
    14581 gcagcttatg acgcgggcgc gatttacggt gggttgattt ttgttgcgac atcaccgcgt
    14641 tgcgtcaacg ttgaacaggc gcaggaagtg atggctgcgg caccgttgca gtatgttggc
    14701 gtgttccgca atcacgatat tgccgatgtg gtggacaaag ctaaggtgtt atcgctggcg
    14761 gcagtgcaac tgcatggtaa tgaagaacag ctgtatatcg atacgctgcg tgaagctctg
    14821 ccagcacatg ttgccatctg gaaagcatta agcgtcggtg aaaccctgcc cgcccgcgag
    14881 tttcagcacg ttgataaata tgttttagac aacggccagg gtggaagcgg gcaacgtttt
    14941 gactggtcac tattaaatgg tcaatcgctt ggcaacgttc tgctggcggg gggcttaggc
    15001 gcagataact gcgtggaagc ggcacaaacc ggctgcgccg gacttgattt taattctgct
    15061 gtagagtcgc aaccgggcat caaagacgca cgtcttttgg cctcggtttt ccagacgctg
    15121 cgcgcatatt aaggaaagga acaatgacaa cattacttaa cccctatttt ggtgagtttg
    15181 gcggcatgta cgtgccacaa atcctgatgc ctgctctgcg ccagctggaa gaagcttttg
    15241 tcagtgcgca aaaagatcct gaatttcagg ctcagttcaa cgacctgctg aaaaactatg
    15301 ccgggcgtcc aaccgcgctg accaaatgcc agaacattac agccgggacg aacaccacgc
    15361 tgtatctcaa gcgtgaagat ttgctgcacg gcggcgcgca taaaactaac caggtgctgg
    15421 ggcaggcgtt gctggcgaag cggatgggta aaaccgaaat catcgccgaa accggtgccg
    15481 gtcagcatgg cgtggcgtcg gcccttgcca gcgccctgct cggcctgaaa tgccgtattt
    15541 atatgggtgc caaagacgtt gaacgccagt cgcctaacgt ttttcgtatg cgcttaatgg
    15601 gtgcggaagt gatcccggtg catagcggtt ccgcgacgct gaaagatgcc tgtaacgagg
    15661 cgctgcgcga ctggtccggt agttacgaaa ccgcgcacta tatgctgggc accgcagctg
    15721 gcccgcatcc ttatccgacc attgtgcgtg agtttcagcg gatgattggc gaagaaacca
    15781 aagcgcagat tctggaaaga gaaggtcgcc tgccggatgc cgttatcgcc tgtgttggcg
    15841 gcggttcgaa tgccatcggc atgtttgctg atttcatcaa tgaaaccaac gtcggcctga
    15901 ttggtgtgga gccaggtggt cacggtatcg aaactggcga gcacggcgca ccgctaaaac
    15961 atggtcgcgt gggtatctat ttcggtatga aagcgccgat gatgcaaacc gaagacgggc
    16021 agattgaaga atcttactcc atctccgccg gactggattt cccgtctgtc ggcccacaac
    16081 acgcgtatct taacagcact ggacgcgctg attacgtgtc tattaccgat gatgaagccc
    16141 ttgaagcctt caaaacgctg tgcctgcacg aagggatcat cccggcgctg gaatcctccc
    16201 acgccctggc ccatgcgttg aaaatgatgc gcgaaaaccc ggataaagag cagctactgg
    16261 tggttaacct ttccggtcgc ggcgataaag acatcttcac cgttcacgat attttgaaag
    16321 cacgagggga aatctgatgg aacgctacga atctctgttt gcccagttga aggagcgcaa
    16381 agaaggcgca ttcgttcctt tcgtcacgct cggtgatccg ggcattgagc agtcattgaa
    16441 aattatcgat acgctaattg aagccggtgc tgacgcgctg gagttaggta tccccttctc
    16501 cgacccactg gcggatggcc cgacgattca aaacgccact ctgcgcgcct ttgcggcagg
    16561 tgtgactccg gcacaatgtt ttgaaatgct ggcactgatt cgccagaaac acccgaccat
    16621 tcccattggc ctgttgatgt atgccaatct ggtgtttaac aaaggcattg atgagtttta
    16681 tgcccagtgc gaaaaagtcg gcgtcgattc ggtgctggtt gccgatgtgc cagttgaaga
    16741 gtccgcgccc ttccgccagg ccgcgttgcg tcataatgtc gcacctatct tcatctgccc
    16801 gccaaatgcc gatgacgacc tgctgcgcca gatagcctct tacggtcgtg gttacaccta
    16861 tttgctgtca cgagcaggcg tgaccggcgc agaaaaccgc gccgcgttac ccctcaatca
    16921 tctggttgcg aagctgaaag agtacaacgc tgcacctcca ttgcagggat ttggtatttc
    16981 cgccccggat caggtaaaag cagcgattga tgcaggagct gcgggcgcga tttctggttc
    17041 ggccattgtt aaaatcatcg agcaacatat taatgagcca gagaaaatgc tggcggcact
    17101 gaaagttttt gtacaaccga tgaaagcggc gacgcgcagt taatcccaca gccgccagtt
    17161 ccgctggcgg cattttaact ttctttaatg aagccggaaa aatcctaaat tcatttaata
    17221 tttatctttt taccgtttcg cttaccccgg tcgaacgtca acttacgtca tttttccgcc
    17281 caacagtaat ataatcaaac aaattaatcc cgcaacataa caccagtaaa atcaataatt
    17341 ttctctaagt cacttattcc tcaggtaatt gttaatatat ccagaatgtt cctcaaaata
    17401 tattttccct ctatcttctc gttgcgctta atttgactaa ttctcattag cgactaattt
    17461 taatgagtgt cgaggatccc cgggtacccg gggatcctct agaactagg
//
