LOCUS       .                       4849 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(10..759)
                     /gene="luxR"
                     /label="luxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             986..1018
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      1060..1146
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1184..1933)
                     /gene="luxR"
                     /label="luxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             2160..2738
                     /gene="luxI"
                     /label="luxI"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             2739..2771
                     /label="ssrA tag"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     terminator      complement(2795..2838)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(3043..3631)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(3719..3813)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3847..4638)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgagttaa tttttaaagt atgggcaatc aattgctcct gttaaaattg ctttagaaat
       61 actttggcag cggtttgttg tattgagttt catttgcgca ttggttaaat ggaaagtgac
      121 agtacgctca ctgcagccta atatttttga aatatcccaa gagctttttc cttcgcatgc
      181 ccacgctaaa cattcttttt ctcttttggt taaatcgttg tttgatttat tatttgctat
      241 atttattttt cgataattat caactagaga aggaacaatt aatggtatgt tcatacacgc
      301 atgtaaaaat aaactatcta tatagttgtc tttttctgaa tgtgcaaaac taagcattcc
      361 gaagccattg ttagccgtat gaatagggaa actaaaccca gtgataagac ctgatgtttt
      421 cgcttcttta attacatttg gagatttttt atttacagca ttgttttcaa atatattcca
      481 attaattggt gaatgattgg agttagaata atctactata ggatcatatt ttattaaatt
      541 agcgtcatca taatattgcc tccatttttt agggtaatta tctagaattg aaatatcaga
      601 tttaaccata gaatgaggat aaatgatcgc gagtaaataa tattcacaat gtaccatttt
      661 agtcatatca gataagcatt gattaatatc attattgctt ctacaagctt taattttatt
      721 aattattctg tatgtgtcgt cggcatttat gtttttcata cccatctctt tatccttacc
      781 tattgtttgt cgcaagtttt gcgtgttata tatcattaaa acggtaatgg attgacattt
      841 gattctaata aattggattt ttgtcacact attgtatcgc tgggaataca attacttaac
      901 ataagcacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaatga
      961 attcattaaa gaggagaaag gtaccgcagc gaacgacgaa aattacgccc ttgcagcgta
     1021 aaagcttaat tagctgatct agacgcgtgc tagaggcatc aaataaaacg aaaggctcag
     1081 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     1141 acaaatccgc cgccctagac ctagccgtct tcacctcgag ttaattttta aagtatgggc
     1201 aatcaattgc tcctgttaaa attgctttag aaatactttg gcagcggttt gttgtattga
     1261 gtttcatttg cgcattggtt aaatggaaag tgacagtacg ctcactgcag cctaatattt
     1321 ttgaaatatc ccaagagctt tttccttcgc atgcccacgc taaacattct ttttctcttt
     1381 tggttaaatc gttgtttgat ttattatttg ctatatttat ttttcgataa ttatcaacta
     1441 gagaaggaac aattaatggt atgttcatac acgcatgtaa aaataaacta tctatatagt
     1501 tgtctttttc tgaatgtgca aaactaagca ttccgaagcc attgttagcc gtatgaatag
     1561 ggaaactaaa cccagtgata agacctgatg ttttcgcttc tttaattaca tttggagatt
     1621 ttttatttac agcattgttt tcaaatatat tccaattaat tggtgaatga ttggagttag
     1681 aataatctac tataggatca tattttatta aattagcgtc atcataatat tgcctccatt
     1741 ttttagggta attatctaga attgaaatat cagatttaac catagaatga ggataaatga
     1801 tcgcgagtaa ataatattca caatgtacca ttttagtcat atcagataag cattgattaa
     1861 tatcattatt gcttctacaa gctttaattt tattaattat tctgtatgtg tcgtcggcat
     1921 ttatgttttt catacccatc tctttatcct tacctattgt ttgtcgcaag ttttgcgtgt
     1981 tatatatcat taaaacggta atggattgac atttgattct aataaattgg atttttgtca
     2041 cactattgta tcgctgggaa tacaattact taacataagc acctgtagga tcgtacaggt
     2101 ttacgcaaga aaatggtttg ttatagtcga atgaattcat taaagaggag aaaggtacca
     2161 tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta
     2221 ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa
     2281 ataaccttga atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg
     2341 atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga
     2401 aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg
     2461 aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa
     2521 ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat
     2581 atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc
     2641 atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta
     2701 tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc agcgaacgac gaaaattacg
     2761 cccttgcagc gtaaacgcgt gctagaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2821 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     2881 gccgccctag acctagggcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2941 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3001 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3061 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgacta gtgcttggat
     3721 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     3781 ctgaggtcat tactggatct atcaacagga gtccaagcga gctctcgaac cccagagtcc
     3841 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     3901 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     3961 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     4021 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     4081 cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg
     4141 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     4201 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     4261 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     4321 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     4381 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     4441 ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     4501 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     4561 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     4621 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc
     4681 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
     4741 aaccttacca gagggcgccc cagctggcaa ttccgacgtc taagaaacca ttattatcat
     4801 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcac
//
