LOCUS       V043818                 7652 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043818
VERSION     V043818
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..1179
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     promoter        1196..1214
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     protein_bind    1293..1317
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB1"
     CDS             1348..3552
                     /label="Slc26a6(NM_134420)"
                     /note="Slc26a6(NM_134420)"
                     /gene="Slc26a6"
     polyA_signal    3580..3804
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     protein_bind    3874..3907
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
                     /label="loxP"
     primer_bind     complement(4365..4381)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     promoter        4855..4959
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             4960..5820
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      5991..6579
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    6867..6888
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        6903..6933
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6941..6957
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     6965..6981
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7479..7512
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
                     /label="loxP"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctccg
     1321 cggccgcccc cttcaccatg ggccaccatg gagctgcaga ggagagacta ccatgtggaa
     1381 aggccattgc tgaaccagga gcagctagaa gatctggggc attggggccc agcggcgaag
     1441 acccaccagt ggcgaacttg gttccgatgc tcccgtgctc gggcccattc tcttctgctc
     1501 cagcacgttc cagtcctggg ctggttaccc cggtatcctg tgcgtgaatg gctcctgggt
     1561 gatctgttat ctggcctgag tgtggccatc atgcagcttc ctcagggctt ggcctatgcc
     1621 cttctggcgg gattgcctcc tatgtttggt ctgtacagtt ctttctaccc cgtcttcatc
     1681 tacttcctgt ttggtacctc cagacacatc tctgtgggga cctttgctgt aatgtctgtg
     1741 atggtgggca gtgtgacaga atctctgaca gcagataagg ccttcgtgca aggcttgaat
     1801 gccacagctg atgatgcacg cgtgcaggtg gcctacacac tcagcttcct ggtcggcctc
     1861 ttccaggtgg ggctgggcct ggtacacttc ggcttcgttg tcacctacct gtcagagcct
     1921 ctggtccgca gctataccac ggctgcatct gtgcaagtcc tcgtctctca gctcaagtat
     1981 gtgtttggca tcaaactcag cagtcactct gggccactgt ccgttatcta tacagtgctg
     2041 gaggtctgtg cccagctccc tgagactgtg cccggcaccg tggtcacggc gattgtggca
     2101 ggagtggcct tggtactggt gaagctactt aatgagaagc tgcatcggcg tctgcccctg
     2161 cccatccctg gggaactact cacgctcatt ggggccactg gtatttccta tggtgtgaag
     2221 ctgaatgaca gattcaaggt cgatgtggtg ggcaacatca ccacagggct catacccccg
     2281 gtggcaccca agacagagct gtttgcaacg cttgtgggaa atgcctttgc cattgctgtg
     2341 gtgggcttcg ccattgccat ctcactgggg aagatctttg ccctgaggca tggctaccgt
     2401 gtggacagta accaggagct ggtagccctt ggcctcagta acctcattgg aggcttcttc
     2461 cagtgcttcc ccgtgagctg ctccatgtct cggagcttgg tacaggagag cacgggaggc
     2521 aacacacagg ttgctggagc tgtatcttcc ctttttatcc ttcttattat cgtcaaactt
     2581 ggggaactct tccgagacct gcccaaggcc gtcctggctg ctgtcattat tgtgaaccta
     2641 aagggcatga tgaagcagtt ctcagacatc tgctctcttt ggaaggcaaa cagagtggac
     2701 ctgctaatct ggctggtgac ctttgtggcc acaatcctgc tgaacctgga cattggcctg
     2761 gcagtttcca tagtcttctc cttgctgctc gtggtcgtcc gaatgcagct gccccattac
     2821 tccgtcctgg ggcaggtgcc agatacggat atttatagag acgtggcaga atactctggg
     2881 gccaaggagg tcccgggtgt gaaagtcttc cgttcctcag ccacgctgta cttcgccaat
     2941 gctgagctct acagcgactc tctgaaagag aagtgcggtg tagatgttga ccgcctcatc
     3001 acccagaaga agaaacgaat caaaaagcag gagatgaagt taaagcgaat gaagaaagcc
     3061 aagaagtccc agaaacagga tgcttcttcc aagatctcct cagtttccgt caacgtcaac
     3121 accaacttgg aagacgtcaa aagcaatgac gtcgagggct ctgaggccaa ggtgcaccaa
     3181 ggggaggagc ttcaggatgt agtctccagc aatcaagaag atgccaaggc cccaaccatg
     3241 acctcactga agtccctggg cctgcctcag ccaggcttcc atagcctcat cctggacctg
     3301 agcaccctct cctttgtgga cactgtgtgc attaagagcc tgaagaatat tttccgtgac
     3361 ttccgggaga ttgaagtgga agtgtacatc gcagcctgtt acagtcctgt ggtcgcccag
     3421 cttgaagctg gacacttctt tgatgaatct atcactaagc agcatgtctt tgcctctgtc
     3481 catgacgctg tgacctttgc cctcagccac cggaagtctg tccctaagag ccctgttttg
     3541 gccaccaaac tctgatttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3601 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3661 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3721 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3781 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3841 ggtatcccca cgcgccctgt agcggcgcat taaataactt cgtataatgt atgctatacg
     3901 aagttatatc gtatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     3961 acaccctaac tgacacacat tccacagaat tccttgaaat acatccacag gtacacctcc
     4021 aattgactca aatgatgtca attagtctat cagaagcttc taaagccatg acatcatttt
     4081 ctggaatttt ccaagctgtt taaaggcaca gtcaacttag tgtatgtaaa cttctgaccc
     4141 actggaattg tgatacagtg aattataagt gaaataatct gtctgtaaac aattgttgga
     4201 aaaatgactt gtgtcatgca caaagtagat gtcctaactg acttgccaaa actattgttt
     4261 gttaacaaga aatttgtgga gtagttgaaa aacgagtttt aatgactcca acttaagtgt
     4321 atgtaaactt ccgacttcaa ctgtagggta ccgagctcga attcactggc cgtcgtttta
     4381 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4441 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4501 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4561 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4621 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     4681 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     4741 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4801 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4861 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4921 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4981 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5041 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5101 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5161 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5221 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5281 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5341 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5401 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5461 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5521 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5581 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5641 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5701 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5761 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5821 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5881 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5941 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6001 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6061 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6121 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6181 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6241 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6301 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6361 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6421 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6481 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6541 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6601 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6661 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6721 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6781 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6841 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6901 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6961 cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca ggtcgactct
     7021 agacctatac agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg
     7081 tttttcaact actccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg
     7141 acatctactt tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt
     7201 tcacttataa ttcactgtat cacaattcca gtgggtcaga agtttacata cactaagttg
     7261 actgtgcctt taaacagctt ggaaaattcc agaaaatgat gtcatggctt tagaagcttc
     7321 tgatagacta attgacatca tttgagtcaa ttggaggtgt acctgtggat gtatttcaag
     7381 gggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagc
     7441 ggccgctagc tcgagatctt aattaaacta gtatcgatat aacttcgtat aatgtatgct
     7501 atacgaagtt atatgtacgg gccagatata cgcgttgaca ttgattattg actaggcttt
     7561 tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
     7621 ggaccttcta ggtcttgaaa ggagtgggaa tt
//
