LOCUS       62056_20480        6123 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        365..668
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        671..946
                     /label=chicken beta-actin promoter
     intron          947..1955
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1976..2692
                     /codon_start=1
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2708..2829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3231..3249)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3259..3275)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(3416..3871)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3898..4002
                     /label=AmpR promoter
     CDS             4003..4860
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5034..5622
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5910..5931
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5946..5976
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5984..6000
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6008..6024
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6045..6063
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tatacagttg aagtcggaag tttacataca ccttagccaa atacatttaa actcactttt
       61 tcacaattcc tgacatttaa tcctagtaaa aattccctgt cttaggtcag ttaggatcac
      121 cactttattt taagaatgtg aaatatcaga ataatagtag agagaatgat tcatttcagc
      181 ttttatttct ttcatcacat tcccagtggg tcagaagttt acatacactc aattagtatt
      241 tggtagcatt gcctttaaat tgtttaactt gggtcaaaca tttcgagtag ccttccacaa
      301 gctctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      361 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      421 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      481 gtcaatgggt ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      541 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      601 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      661 ttaccatggg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
      721 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
      781 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
      841 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
      901 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt
      961 gccttcgccc cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac
     1021 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     1081 cttggtttaa tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg
     1141 ggagggccct ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
     1201 ggagcgccgc gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     1261 gctttgtgcg ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
     1321 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     1381 ggggtgtggg cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct
     1441 gagcacggcc cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg
     1501 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
     1561 gagggctcgg gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga
     1621 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     1681 aaatctggcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg
     1741 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     1801 cgccgtcccc ttctccatct ccagcctcgg ggctgccgca gggggacggc tgccttcggg
     1861 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     1921 ctaaccatgt tcatgccttc ttctttttcc tacagatcct taattaatct agaccatggt
     1981 gtccaagggc gaggagctgt tcaccggcgt ggtgcccatc ctggtggagc tggacggcga
     2041 cgtgaacggc cacaagttct ccgtgagcgg cgagggcgag ggcgacgcca cctacggcaa
     2101 gctgaccctg aagctgatct gcaccaccgg caagctgcct gtgccctggc ctaccctggt
     2161 gaccaccctg ggctacggcc tgcagtgctt cgccagatac cccgaccaca tgaagcagca
     2221 cgacttcttc aagtccgcca tgcctgaggg ctacgtgcag gagagaacca tcttcttcaa
     2281 ggacgacggc aactacaaga ccagagccga ggtgaagttc gagggcgaca ccctggtgaa
     2341 cagaatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccacaagct
     2401 ggagtacaac tacaacagcc acaacgtgta catcaccgcc gacaagcaga agaacggcat
     2461 caaggccaac ttcaagatca ggcacaacat cgaggacggc ggcgtgcagc tggccgacca
     2521 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2581 gagctaccag agcgccctga gcaaggaccc taacgagaag agggaccaca tggtgctgct
     2641 ggagttcgtg accgccgccg gcatcaccct gggcatggac gagctgtaca agtgagcggc
     2701 cgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2761 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2821 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2881 gttaaatcct cgagtgcgtc cagctagcac gactagagct tctaaagcca tgacatcatt
     2941 ttctggaatt ttccaagctg tttaaaggca cagtcaactt agtgtatgta aacttctgac
     3001 ccactggaat tgtgatacag tgaattataa gtgaaataat ctgtctgtaa acaattgttg
     3061 gaaaaatgac ttgtgtcatg cacaaagtag atgtcctaac tgacttgcca aaactattgt
     3121 ttgttaacaa gaaatttgtg gagtagttga aaaacgagtt ttaatgactc caacttaagt
     3181 gtatgtaaac ttccgacttc aactgtatag ggatccgagc tccaattcgc cctatagtga
     3241 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3301 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3361 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
     3421 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3481 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3541 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3601 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3661 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3721 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3781 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3841 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     3901 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3961 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4021 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4081 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4141 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4201 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4261 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4321 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4381 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4441 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4501 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4561 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4621 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4681 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4741 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4801 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4861 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4921 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4981 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5041 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5101 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5161 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5221 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5281 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5341 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5401 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5461 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5521 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5581 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5641 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5701 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5761 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     5821 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     5881 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     5941 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6001 tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa
     6061 agggaacaaa agctgggtac cgggcccccc ctcgatcgag gtcgactcta gctagaggat
     6121 ccc
//
