LOCUS       40924_41470        4829 bp DNA     circular SYN 18-DEC-2018
DEFINITION  SiRNA vector pSUPER-hSyn-DsRed2N1-CytB-AS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Michel U, Malik I, Ebert S, Bahr M, Kugler S.
  TITLE     Long-term in vivo and in vitro AAV-2-mediated RNA interference in 
            rat retinal ganglion cells and cultured primary neurons
  JOURNAL   Biochem. Biophys. Res. Commun. 326 (2), 307-312 (2005)
  PUBMED    15582578
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Michel U, Kuegler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2004) Neurology, University of Goettingen, Waldweg
            33, Goettingen 37073, Germany
REFERENCE   3  (bases 1 to 4829)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4829)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2005"; volume: "326"; issue: "2"; 
            pages: "307-312"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2004) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen 37073, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     681..697
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(752..886)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(902..1035)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(1048..1722)
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    complement(1741..1759)
                     /note="MCS; multiple cloning site"
     promoter        complement(1765..2212)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     promoter        2240..2454
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    2456..2587
                     /note="Cytb-AS; partial human cytochrome B antisense
                     sequence"
     primer_bind     complement(2595..2611)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2641..2659)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2680..2696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2704..2720)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2728..2758)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2773..2794)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3082..3670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3844..4701)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4702..4806)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccccccct cgaggtcgac ggtatcgata agctagcttg atatcgaatt
      721 cctgcagccc gggggatctt cgatgctaga cgatccagac atgataagat acattgatga
      781 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      841 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttgctc gaagtcgaca
      901 cctgtggaga gaaaggcaaa gtggatgtca gtaagaccaa taggtgccta tcagaaacgc
      961 aagagtcttc tctgtctcga caagcccagt ttctatgtgg tctccttaaa cctgtcttgt
     1021 aaccttgata cttacctgcg gccgctacag gaacaggtgg tggcggccct cggtgcgctc
     1081 gtactgctcc acgatggtgt agtcctcgtt gtgggaggtg atgtccagct tggcgtccac
     1141 gtagtagtag ccgggcagct gcacgggctt cttggccatg tagatggact tgaactccac
     1201 caggtagtgg ccgccgtcct tcagcttcag ggccttgtgg gtctcgccct tcagcacgcc
     1261 gtcgcggggg tacaggcgct cggtggaggc ctcccagccc atggtcttct tctgcatcac
     1321 ggggccgtcg gaggggaagt tcacgccgat gaacttcacc ttgtagatga agcagccgtc
     1381 ctgcagggag gagtcctggg tcacggtcgc cacgccgccg tcctcgaagt tcatcacgcg
     1441 ctcccacttg aagccctcgg ggaaggacag cttcttgtag tcggggatgt cggcggggtg
     1501 cttcacgtac accttggagc cgtactggaa ctggggggac aggatgtccc aggcgaaggg
     1561 cagggggccg cccttggtca ccttcagctt cacggtgttg tggccctcgt aggggcggcc
     1621 ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gtgccctcca tgcgcacctt
     1681 gaagcgcatg aactcggtga tgacgttctc ggaggaggcc atggtggcga ccggtggatc
     1741 cttgctagca gcttgaattc tcgactgcgc tctcaggcac gacacgactc ctccgctgcc
     1801 caccgcagac tgaggcagcg ctgagtcgcc ggcgccgcag cgcagatggt cgcgcccgtg
     1861 cccccctatc tcgcgcctcg cgtggtgcgg tccggctggg ccggcggcgg cgcggacgcg
     1921 accaaggtgg ccgggaaggg gagtttgcgg gggaccggcg agtgacgtca gcgcgccttc
     1981 agtgctgagg cggcggtggc gcgcgccgcc aggcgggggc gaaggcactg tccgcggtgc
     2041 tgaagctggc agtgcgcacg cgcctcgccg catcctgttt cccctccccc tctctgatag
     2101 gggatgcgca atttggggaa tgggggttgg gtgcttgtcc agtgggtcgg ggtcggtcgt
     2161 caggtaggca cccccacccc gcctcatcct ggtcctaaaa cccacttgca ctcatacgca
     2221 gggccctctg caggaattcg aacgctgacg tcatcaaccc gctccaagga atcgcgggcc
     2281 cagtgtcact aggcgggaac acccagcgcg cgtgcgccct ggcaggaaga tggctgtgag
     2341 ggacagggga gtggcgccct gcaatatttg catgtcgcta tgtgttctgg gaaatcacca
     2401 taaacgtgaa atgtctttgg atttgggaat cttataagtt ctgtatgaga ccacagatct
     2461 cccaatgtat gggatggcgg atagtaagtt tgtaattact gtggcccctc agaatgatat
     2521 ttggcctcac gggaggacat agcctatgaa ggctgttgct atagttgcaa gcaggaggat
     2581 aatgccaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca gcttttgttc
     2641 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     2701 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     2761 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2821 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2881 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2941 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3001 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3061 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3481 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     3541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3841 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3901 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3961 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4021 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4081 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4141 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4201 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4261 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4321 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4381 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4441 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4501 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4561 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4621 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4681 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4741 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4801 tccgcgcaca tttccccgaa aagtgccac
//
