LOCUS       V003036                 3810 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003036
VERSION     V003036
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.
  TITLE     Markerless gene replacement in Escherichia coli stimulated by a
            double-strand break in the chromosome
  JOURNAL   Nucleic Acids Res. 27 (22), 4409-4415 (1999)
   PUBMED   10536150
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-1999) Institute of Biochemistry, Biol. Res. Center
            Hung. Acad.Sci., Temesvari krt.,62, Szeged H-6726, Hungary
REFERENCE   3  (bases 1 to 3810)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3810)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1999"; volume: "27"; issue: "22"; pages:
            "4409-4415"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JUL-1999) Institute of Biochemistry, Biol. Res. Center Hung.
            Acad.Sci., Temesvari krt.,62, Szeged H-6726, Hungary"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        57..112
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             126..818
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     CDS             complement(979..1926)
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
     rep_origin      complement(1974..2196)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(2821..3678)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3679..3783)
                     /label="AmpR promoter"
ORIGIN
        1 atcgatgaat tgatccgaag ttcctattct ctagacatca ttaattccta atttttgttg
       61 acactctatc attgatagag ttattttacc actccctatc agtgatagag aaaagtgaaa
      121 tgcatcaaaa aaaccaggta atgaacctgg gtccgaactc taaactgctg aaagaataca
      181 aatcccagct gatcgaactg aacatcgaac agttcgaagc aggtatcggt ctgatcctgg
      241 gtgatgctta catccgttct cgtgatgaag gtaaaaccta ctgtatgcag ttcgagtgga
      301 aaaacaaagc atacatggac cacgtatgtc tgctgtacga tcagtgggta ctgtccccgc
      361 cgcacaaaaa acaacgtgtt aaccacctgg gtaacctggt aatcacctgg ggcgcccaga
      421 ctttcaaaca ccaagctttc aacaaactgg ctaacctgtt catcgttaac aacaaaaaaa
      481 ccatcccgaa caacctggtt gaaaactacc tgaccccgat gtctctggca tactggttca
      541 tggatgatgg tggtaaatgg gattacaaca aaaactctac caacaaatcg atcgtactga
      601 acacccagtc tttcactttc gaagaagtag aatacctggt taagggtctg cgtaacaaat
      661 tccaactgaa ctgttacgta aaaatcaaca aaaacaaacc gatcatctac atcgattcta
      721 tgtcttacct gatcttctac aacctgatca aaccgtacct gatcccgcag atgatgtaca
      781 aactgccgaa cactatctcc tccgaaactt tcctgaaata ataagtcgac tgcaggcatg
      841 caagcttggc actggctgat cagctagccc atgggtatgg acagttttcc ctttgatatg
      901 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca
      961 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg
     1021 ttgtttttgc gtgagccatg agaacgaacc attgagatca tgcttacttt gcatgtcact
     1081 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt
     1141 ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc
     1201 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc
     1261 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt
     1321 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa
     1381 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat
     1441 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat
     1501 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa
     1561 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa
     1621 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc
     1681 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct
     1741 actgatgttc atcatctgaa cgtattggtt ataagtgaac gataccgtcc gttctttcct
     1801 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc
     1861 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc
     1921 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag
     1981 tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac
     2041 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg
     2101 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa
     2161 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta
     2221 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa
     2281 aggcttaagt agcaccctcg caagctcggt tgcggccgca atcgggcaaa tcgctgaata
     2341 ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc
     2401 gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg
     2461 attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt
     2521 tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc
     2581 tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa
     2641 tgcacccagt aaggcagcgg tatcatcaac ggggtctgac gctcagtgga acgaaaactc
     2701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2821 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2881 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2941 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3001 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3061 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3121 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3181 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3241 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3301 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3361 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3421 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3481 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3541 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3601 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3661 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3721 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3781 gcgcacattt ccccgaaaag tgccacttgc
//
