LOCUS Exported 4466 bp DNA circular SYN 27-FEB-2024 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4466) AUTHORS 11111111 TITLE Direct Submission JOURNAL Exported Sep 10, 2025 from SnapGene 6.0.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..4466 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 193..222 /label=trc promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 230..246 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." terminator 820..906 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 998..1025 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 1044..1135 /gene="bla" /label=AmpR promoter CDS 1136..1996 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2167..2755 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 2941..3081 /label=bom /note="basis of mobility region from pBR322" promoter 3267..3344 /gene="lacI (mutant)" /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." CDS 3345..4427 /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" ORIGIN 1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 241 taacaatttc acacaggaaa cagaccatgg ctggtgacca cgtcgtggaa tgccttcgaa 301 ttcagcacct gcacatggga cgtcgacctg aggtaattat aacccgggcc ctatatatgg 361 atccaattgc aatgatcatc atgacagatc tgcgcgcgat cgatatcagc gctttaaatt 421 tgcgcatgct agctatagtt ctagaggtac cggttgttaa cgttagccgg ctacgtatac 481 tccggaatat taataggcct aggatgcata tggcggccgc ctgcagctgg cgccatcgat 541 acgcgtacgt cgcgaccgcg gacatgtaca gagctcgaga agtactagtg gccacgtggg 601 ccgtgcacct taagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga 661 ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg 721 tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg 781 tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag 841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg 901 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca 961 ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc 1021 ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc 1081 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 1141 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 1201 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 1261 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 1321 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt 1381 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 1441 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 1501 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 1561 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 1621 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 1681 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 1741 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 1801 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 1861 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 1921 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 1981 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 2041 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 2101 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 2161 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 2221 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 2281 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 2341 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 2401 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 2461 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 2521 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 2581 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 2641 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 2701 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 2761 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 2821 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 2881 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 2941 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac 3001 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta 3061 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg 3121 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 3181 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg 3241 aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta 3301 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt 3361 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc 3421 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga 3481 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg 3541 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc 3601 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag 3661 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact 3721 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt 3781 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg 3841 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag 3901 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca 3961 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt 4021 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca 4081 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg 4141 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt 4201 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc 4261 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa 4321 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 4381 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 4441 aatgtaagtt agcgcgaatt gatctg //