LOCUS       pRSET-BFP.        3600 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing BFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pRSET-BFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             59..81
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             89..91
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             101..118
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             122..154
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /label=epitope tag from a T7 major capsid protein
                     /note="T7 tag"
                     /translation="MASMTGGQQMG"
     CDS             158..181
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             209..925
                     /label=BFP
                     /note="blue variant of GFP"
     terminator      1004..1051
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1148..1603
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1629..1733
                     /label=AmpR promoter
     CDS             1734..2591
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2765..3353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gcggggttct catcatcatc atcatcatgg
      121 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
      181 ggatcgatgg ggatccgaat tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
      241 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      301 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      361 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgagccacg gcgtccagtg
      421 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      481 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      541 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      601 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaacgt
      661 ctatatcatg gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa
      721 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      781 cggccccgtg ctgctgcccg acagccacta cctgagcacc cagtccgccc tgagcaaaga
      841 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      901 tctcggcatg gacgagctgt acaagtaact cgagaagctt gatccggctg ctaacaaagc
      961 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     1021 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatctgg
     1081 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1141 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1201 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     1261 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     1321 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1381 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1441 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1501 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     1561 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     1621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1741 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1801 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1861 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1921 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1981 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2041 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2101 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2161 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2221 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2281 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2341 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2401 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2461 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2521 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2581 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2641 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2881 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3421 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3481 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3541 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagg atctcgatcc
//
