LOCUS       pRGEB32                15888 bp    DNA     circular SYN 29-DEC-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15888)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 15888)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 15888)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..15888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..49
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     terminator      71..323
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(332..348)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    551..575
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1876..2502
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2940..4004
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      4073..4267
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4611..4751
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4937..5525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5615..6406)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6831..6855
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6933..7107)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7151..8173)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(8240..8916)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9107..9128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9143..9173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9181..9197
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9205..9221
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9239..9619
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        9636..9711
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     intron          10540..11501
                     /label=intron
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     protein_bind    11512..11536
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11581..11646
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=three tandem FLAG
                     /note="3xFLAG"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             11653..11673
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=nuclear localization signal of SV40 large T
                     ant
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             11698..15798
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             15799..15846
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=bipartite nuclear localization signal from
                     nucl
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cttgtacaaa gtggttgata acagcgacta caaggatgac gatgacaagg cttagagctc
       61 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
      121 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
      181 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
      241 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
      301 ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa cgtcgtgact
      361 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      421 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      481 gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
      541 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
      601 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
      661 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
      721 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
      781 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
      841 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
      901 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
      961 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
     1021 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
     1081 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
     1141 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
     1201 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
     1261 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
     1321 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     1381 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     1441 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     1501 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     1561 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     1621 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc
     1681 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     1741 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     1801 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     1861 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     1921 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     1981 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     2041 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     2101 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     2161 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     2221 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     2281 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     2341 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     2401 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     2461 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     2521 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     2581 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     2641 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     2701 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     2761 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     2821 gacgccgtgg aatgccccat gtgttgagga acgggcggtt ggccaggcgt aagcggctgg
     2881 gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     2941 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     3001 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     3061 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     3121 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     3181 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     3241 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     3301 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     3361 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     3421 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     3481 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     3541 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     3601 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     3661 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     3721 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     3781 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     3841 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     3901 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     3961 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     4021 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa agcactcttt cctgtgaata
     4081 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     4141 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     4201 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     4261 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     4321 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     4381 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     4441 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     4501 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     4561 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     4621 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     4681 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4741 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4801 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4861 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4921 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4981 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5041 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5101 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5161 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5221 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5281 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5341 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5401 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5461 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5521 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5581 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
     5641 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
     5701 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
     5761 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
     5821 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
     5881 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
     5941 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
     6001 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
     6061 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
     6121 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
     6181 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
     6241 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
     6301 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
     6361 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
     6421 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
     6481 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
     6541 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
     6601 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
     6661 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
     6721 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
     6781 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
     6841 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
     6901 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
     6961 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     7021 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg
     7081 aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat cccggtcggc
     7141 atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     7201 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     7261 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     7321 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     7381 acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     7441 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     7501 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     7561 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     7621 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     7681 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     7741 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     7801 ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt
     7861 caggcaggtc ttgcaacgtg acaccctgtg aacggcggga gatgcaatag gtcaggctct
     7921 cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     7981 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     8041 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     8101 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     8161 caggcttttt catatctcat tgccccccgg atctgcgaaa gctcgagaga gatagatttg
     8221 tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag
     8281 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     8341 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     8401 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
     8461 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
     8521 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
     8581 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
     8641 cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga cgtggttgga
     8701 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     8761 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
     8821 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     8881 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     8941 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc
     9001 tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     9061 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg ccaagcttaa
     9241 ggaatcttta aacatacgaa cagatcactt aaagttcttc tgaagcaact taaagttatc
     9301 aggcatgcat ggatcttgga ggaatcagat gtgcagtcag ggaccatagc acaagacagg
     9361 cgtcttctac tggtgctacc agcaaatgct ggaagccggg aacactgggt acgttggaaa
     9421 ccacgtgatg tgaagaagta agataaactg taggagaaaa gcatttcgta gtgggccatg
     9481 aagcctttca ggacatgtat tgcagtatgg gccggcccat tacgcaattg gacgacaaca
     9541 aagactagta ttagtaccac ctcggctatc cacatagatc aaagctgatt taaaagagtt
     9601 gtgcagatga tccgtggcag gagaccgagg tctcggtttt agagctagaa atagcaagtt
     9661 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt
     9721 ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcatgcctg
     9781 caggtccaca aattcgggtc aaggcggaag ccagcgcgcc accccacgtc agcaaatacg
     9841 gaggcgcggg gttgacggcg tcacccggtc ctaacggcga ccaacaaacc agccagaaga
     9901 aattacagta aaaaaaaagt aaattgcact ttgatccacc ttttattacc taagtctcaa
     9961 tttggatcac ccttaaacct atcttttcaa tttgggccgg gttgtggttt ggactaccat
    10021 gaacaacttt tcgtcatgtc taacttccct ttcagcaaac atatgaacca tatatagagg
    10081 agatcggccg tatactagag ctgatgtgtt taaggtcgtt gattgcacga gaaaaaaaaa
    10141 tccaaatcgc aacaatagca aatttatctg gttcaaagtg aaaagatatg tttaaaggta
    10201 gtccaaagta aaacttatag ataataaaat gtggtccaaa gcgtaattca ctcaaaaaaa
    10261 atcaacgaga cgtgtaccaa acggagacaa acggcatctt ctcgaaattt cccaaccgct
    10321 cgctcgcccg cctcgtcttc ccggaaaccg cggtggtttc agcgtggcgg attctccaag
    10381 cagacggaga cgtcacggca cgggactcct cccaccaccc aaccgccata aataccagcc
    10441 ccctcatctc ctctcctcgc atcagctcca cccccgaaaa atttctcccc aatctcgcga
    10501 ggctctcgtc gtcgaatcga atcctctcgc gtcctcaagg tacgctgctt ctcctctcct
    10561 cgcttcgttt cgattcgatt tcggacgggt gaggttgttt tgttgctaga tccgattggt
    10621 ggttagggtt gtcgatgtga ttatcgtgag atgtttaggg gttgtagatc tgatggttgt
    10681 gatttgggca cggttggttc gataggtgga atcgtggtta ggttttggga ttggatgttg
    10741 gttctgatga ttggggggaa tttttacggt tagatgaatt gttggatgat tcgattgggg
    10801 aaatcggtgt agatctgttg gggaattgtg gaactagtca tgcctgagtg attggtgcga
    10861 tttgtagcgt gttccatctt gtaggccttg ttgcgagcat gttcagatct actgttccgc
    10921 tcttgattga gttattggtg ccatgggttg gtgcaaacac aggctttaat atgttatatc
    10981 tgttttgtgt ttgatgtaga tctgtagggt agttcttctt agacatggtt caattatgta
    11041 gcttgtgcgt ttcgatttga tttcatatgt tcacagatta gataatgatg aactctttta
    11101 attaattgtc aatggtaaat aggaagtctt gtcgctatat ctgtcataat gatctcatgt
    11161 tactatctgc cagtaattta tgctaagaac tatattagaa tatcatgtta caatctgtag
    11221 taatatcatg ttacaatctg tagttcatct atataatcta ttgtggtaat ttctttttac
    11281 tatctgtgtg aagattattg ccactagttc attctactta tttctgaagt tcaggatacg
    11341 tgtgctgtta ctacctatct gaatacatgt gtgatgtgcc tgttactatc tttttgaata
    11401 catgtatgtt ctgttggaat atgtttgctg tttgatccgt tgttgtgtcc ttaatcttgt
    11461 gctagttctt accctatctg tttggtgatt atttcttgca gatagttatc aacaagtttg
    11521 tacaaaaaag caggcttcga aggagataga accaattctc taaggaaata cttaaccatg
    11581 gactataagg accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac
    11641 gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac
    11701 aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc
    11761 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
    11821 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
    11881 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
    11941 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
    12001 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
    12061 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg
    12121 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
    12181 aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac
    12241 aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac
    12301 gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg
    12361 gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt
    12421 gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc
    12481 aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc
    12541 ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg
    12601 agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc
    12661 aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag
    12721 ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac
    12781 attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag
    12841 atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag
    12901 cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt
    12961 ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag
    13021 atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc
    13081 gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg
    13141 gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg
    13201 cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac
    13261 gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc
    13321 gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag
    13381 cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc
    13441 gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag
    13501 gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc
    13561 ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg
    13621 ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg
    13681 agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc
    13741 ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg
    13801 acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag
    13861 cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag
    13921 gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa
    13981 atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag
    14041 cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa
    14101 aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg
    14161 tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg
    14221 cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag
    14281 aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac
    14341 tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag
    14401 gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg
    14461 gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag
    14521 tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg
    14581 gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac
    14641 cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct
    14701 aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc
    14761 gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc
    14821 atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg
    14881 atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc
    14941 gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca
    15001 ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga
    15061 aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct
    15121 gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag
    15181 ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg
    15241 gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc
    15301 ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag
    15361 ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat
    15421 gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac
    15481 aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg
    15541 gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc
    15601 agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc
    15661 gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg
    15721 ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg
    15781 tctcagctgg gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag
    15841 aaaaagtaag aattcgcggc cgcactcgag atatctagac ccagcttt
//
