LOCUS       62056_16880        6470 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(551..2200)
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     promoter        complement(2225..2428)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2429..2732)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(2854..2872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2884..2902)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2922..2938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2946..2962)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2970..3000)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3015..3036)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3324..3912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4086..4943)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4944..5048)
                     /label=AmpR promoter
     polyA_signal    complement(5136..5217)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5450..6046)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(6143..6459)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctagcggcc gcatacaaaa
      421 aaccaacaca cagatccaat gaaaataaaa gatcctttat taagcttaac tgtttaaacc
      481 tatgatatca cgcacgcgtc tatgccggcc tatgagctcc ctcactagtg cgcagctttc
      541 atcactcgag caatttggac tttccgccct tcttggcctt tatgaggatc tctctgattt
      601 ttcttgcgtc gagttttccg gtaagacctt tcggtacttc gtccacaaac acaactcctc
      661 cgcgcaactt tttcgcggtt gttacttgac tggcgacgta atccacgatc tctttttccg
      721 tcatcgtctt tccgtgctcc aaaacaacaa cggcggcggg aagttcaccg gcgtcatcgt
      781 cgggaagacc tgccacgccc gcgtcgaaga tgttggggtg ttgtaacaat atcgattcca
      841 attcagcggg ggccacctga tatcctttgt atttaattaa agacttcaag cggtcaacta
      901 tgaagaagtg ttcgtcttcg tcccagtaag ctatgtctcc agaatgtagc catccatcct
      961 tgtcaatcaa ggcgttggtc gcttccggat tgtttacata accggacata atcataggtc
     1021 ctctgacaca taattcgcct ctctgattaa cgcccagcgt tttcccggta tccagatcca
     1081 caaccttcgc ttcaaaaaat ggaacaactt taccgaccgc gcccggttta tcatccccct
     1141 cgggtgtaat cagaatagct gatgtagtct cagtgagccc atatccttgt cgtatccctg
     1201 gaagatggaa gcgttttgca accgcttccc cgacttcttt cgaaagaggt gcgcccccag
     1261 aagcaatttc gtgtaaatta gataaatcgt atttgtcaat cagagtgctt ttggcgaaga
     1321 atgaaaatag ggttggtact agcaacgcac tttgaatttt gtaatcctga agggatcgta
     1381 aaaacagctc ttcttcaaat ctatacatta agacgactcg aaatccacat atcaaatatc
     1441 cgagtgtagt aaacattcca aaaccgtgat ggaatgggac aacacttaaa atcgcagtat
     1501 ccggaacgat ttgattgcca aaaataggat ctctggcatg cgagaatctg acgcaggcag
     1561 ttctatgcgg aagggccaca cccttaggta acccagtaga tccagaggaa ttcattatca
     1621 gtgcaattgt tttgtcacga tcaaaggact ctggtacaaa atcgtattca ttaaaaccgg
     1681 gaggtagatg agatgtgacg aacgtgtaca tcgactgaaa tccctggtaa tccgttttag
     1741 aatccatgat aataattttc tggattattg gtaatttttt ttgcacgttc aaaatttttt
     1801 gcaacccctt tttggaaaca aacactacgg taggctgcga aatgttcata ctgttgagca
     1861 attcacgttc attataaatg tcgttcgcgg gcgcaactgc aactccgata aataacgcgc
     1921 ccaacaccgg cataaagaat tgaagagagt tttcactgca tacgacgatt ctgtgatttg
     1981 tattcagccc atatcgtttc atagcttctg ccaaccgaac ggacatttcg aagtattccg
     2041 cgtacgtgat gttcacctcg atatgtgcat ctgtaaaagc aattgttcca ggaaccaggg
     2101 cgtatctctt catagcctta tgcagttgct ctccagcggt tccatcctct agaggataga
     2161 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggatcca cggttcacta
     2221 aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     2281 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     2341 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     2401 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     2461 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     2521 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     2581 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     2641 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     2701 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     2761 atgaccccgt aattgattac tattaataac taagatctgg taccttgaat tcatgcttct
     2821 cctcccttta gtgagggtaa ttctctctct ctccctatag tgagtcgtat taattccttc
     2881 tcttctatag tgtcacctaa atcgttgcaa ttcgtaatca tgtcatagct gtttcctgtg
     2941 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3001 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3061 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3121 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3721 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3841 aaaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3961 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4021 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4081 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4141 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4201 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4261 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4321 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4381 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4441 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4501 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4561 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4621 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4681 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4741 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4801 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4861 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4921 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4981 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5041 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     5101 acattaacct ataaaaatag gcgtatcacg agattgcagt gaaaaaaatg ctttatttgt
     5161 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     5221 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     5281 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga gtcggtgggc
     5341 ctcgggggcg ggtgcggggt cggcggggcc gccccgggtg gcttcggtcg gagccatggg
     5401 gtcgtgcgct cctttcggtc gggcgctgcg ggtcgtgggg cgggcgtcag gcaccgggct
     5461 tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga
     5521 agccgagccg ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg aaggcgggca
     5581 ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc agacccttgc
     5641 cctggtggtc gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt
     5701 gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg ctcaactcgg
     5761 ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc
     5821 agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg agcccgacgc
     5881 gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc gggtcgacgg
     5941 tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg gcccggggga
     6001 cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggaa ggtcgtctcc
     6061 ttgtgagggg tcaggggcgt gggtcagggg atggtggcgg caccggtcgt ggcggccgac
     6121 ctgcaggcat gcaagctagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact
     6181 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     6241 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     6301 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     6361 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     6421 tacttctgcc tgctggggag cctggggact ttccacacca atctttcgtc
//
