LOCUS       V000998                10127 bp    DNA     circular SYN 13-JAN-2022
DEFINITION  Exported.
ACCESSION   V000998
VERSION     V000998
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        1816..2119
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2440..2464
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2496..5540
                     /label="lacZ"
                     /note="beta-galactosidase"
     protein_bind    complement(5560..5584)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5637..5678
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        5720..6049
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        6097..6144
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             6163..6558
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             6648..6881
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6953..7087
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7114..7249
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7270..7288)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7298..7314)
                     /label="M13 fwd"
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      7456..7911
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7937..8041
                     /label="AmpR promoter"
     CDS             8042..8899
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      9073..9661
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9949..9970
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9985..10015
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10023..10039
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10047..10063
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        10084..10102
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca
     2461 ggctccgcgg ccgccccttc accatgatag atcccgtcgt tttacaacgt cgtgactggg
     2521 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     2581 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     2641 aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg
     2701 atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg
     2761 cgcccatcta caccaacgta acctatccca ttacggtcaa tccgccgttt gttcccacgg
     2821 agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag
     2881 gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc
     2941 gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt
     3001 tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac ggcagttatc
     3061 tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata
     3121 aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc
     3181 gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa
     3241 cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg
     3301 aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa
     3361 acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca
     3421 ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga
     3481 ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc
     3541 acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc
     3601 tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc
     3661 tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa
     3721 cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga
     3781 gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt
     3841 cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat
     3901 ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg
     3961 atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga
     4021 aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg
     4081 aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc
     4141 gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta
     4201 aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga
     4261 acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc
     4321 tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg
     4381 aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg
     4441 cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta
     4501 aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca
     4561 cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct
     4621 ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca
     4681 tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc
     4741 aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc
     4801 tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg
     4861 aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc
     4921 aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta
     4981 cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc
     5041 ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac
     5101 cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact
     5161 ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc
     5221 gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc
     5281 tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt
     5341 tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc
     5401 acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg
     5461 actcctggag cccgtcagta tcggcggagt tccagctgag cgccggtcgc taccattacc
     5521 agttggtctg gtgtcaaaaa actaagggtg ggcgcgccga cccagctttc ttgtacaaag
     5581 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta
     5641 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag taatgagttt
     5701 ggaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5761 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5821 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5881 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5941 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6001 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6061 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     6121 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct
     6181 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     6241 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     6301 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     6361 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     6421 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     6481 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     6541 ggttatgtgt gggagggcta agcacaattc gagctcggta cctttaagac caatgactta
     6601 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6661 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6721 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6781 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6841 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     6901 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     6961 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7021 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7081 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7141 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7201 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     7261 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     7321 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     7381 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     7441 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7501 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7561 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7621 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7681 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7741 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7801 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7861 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     7921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9781 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9841 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9901 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9961 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    10021 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
    10081 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagctt
//
