LOCUS       V029086                 9162 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029086
VERSION     V029086
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        65..368
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        369..572
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     protein_bind    595..619
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB1"
     CDS             619..2061
                     /label="OTUD1(NM_001145373)"
                     /note="OTUD1(NM_001145373)"
                     /gene="OTUD1"
     protein_bind    complement(2060..2084)
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB2"
     CDS             2086..2127
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     misc_feature    2169..2757
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             2829..3062
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    3140..3261
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      3301..3436
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(3457..3475)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(3485..3501)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      3643..4098
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4124..4228
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             4229..5089
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      5260..5848
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    6136..6157
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        6172..6202
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6210..6226
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     6234..6250
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        6271..6289
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        6317..6543
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             6544..6724
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    6771..6896
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    7389..7622
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             7807..7851
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     promoter        8026..8526
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     CDS             8538..8933
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
                     /label="BSD"
     misc_feature    8993..9110
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagttggcat gcagctctac agcagcgtct gcacccacta ccccgccggg
      661 gccccgggtc ccacggccgc cgcccccgcg ccacccgccg ccgctacccc cttcaaggtc
      721 tcgctgcagc ccccgggagc cgccggcgcc gcgcccgagc ccgagaccgg tgagtgccag
      781 cccgccgcgg ccgccgagca ccgggaagcc gccgctgtcc ccgccgccaa gatgcccgcc
      841 ttctcctcct gcttcgaggt ggtgtccggg gccgccgcgc ccgcctccgc cgccgccggc
      901 ccgcccggcg cgtcctgcaa gccgccgctg ccgccgcact acacgtccac cgcacagatc
      961 accgtgcggg ccctgggcgc cgacaggctc ctgctgcacg ggcccgatcc cgttcccggc
     1021 gccgcgggct ccgccgctgc cccgcgcggc cgctgcctcc tgctcgcccc ggcgcccgca
     1081 gccccggtcc cgccgcggcg gggctcctcg gcctggctcc tggaggagct gctgcggccc
     1141 gactgccccg agcccgcggg cttggacgcg acacgggagg ggcccgatcg gaacttccga
     1201 ctgagcgagc accgccaggc cctggccgcc gccaagcacc gaggccccgc ggcgaccccg
     1261 gggagccccg atcccggccc cggtccgtgg ggcgaagagc acttggcgga gaggggcccc
     1321 aggggctggg agaggggcgg cgatcgctgc gacgcgcccg gtggggacgc ggcgcggagg
     1381 cccgacccag aggccgaggc accccccgcc gggagcatcg aggccgcccc gagtagtgcg
     1441 gcggagccgg tgatcgtctc caggtcggat cccagagacg agaagctggc cctatacctg
     1501 gccgaggtgg agaagcagga caagtatctg cggcagagga ataagtaccg attccacatc
     1561 attccagacg gcaactgcct ctaccgagct gtcagcaaga cggtgtatgg ggaccagagc
     1621 ctgcaccggg agttgaggga gcagacggtg cactacatcg ccgaccatct cgaccacttc
     1681 agccccctga ttgagggcga cgtgggggag tttatcatcg ctgctgccca agacggggca
     1741 tgggccgggt acccggagtt gctggccatg gggcagatgc tgaatgtgaa tatccattta
     1801 actactggag ggaggctgga gagtcccacg gtgtctacca tgattcatta tttgggccca
     1861 gaggattccc tgaggcctag tatttggctc agttggctca gtaacggaca ctatgatgct
     1921 gtatttgatc actcctatcc taacccagag tacgacaact ggtgcaaaca aactcaagtg
     1981 caaaggaaac gcgacgaaga acttgccaaa tctatggcca tatccttgtc taaaatgtat
     2041 attgaacaaa atgcatgctc tccaactttc ttgtacaaag tggttggtaa gcctatccct
     2101 aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt
     2161 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2221 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2281 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2341 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2401 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2461 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2521 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2581 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2641 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     2701 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     2761 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     2821 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     2881 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     2941 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     3001 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     3061 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     3121 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     3181 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3241 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     3301 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3361 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3421 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     3481 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     3541 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     3601 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     3661 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3721 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3781 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3841 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3901 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3961 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4021 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4081 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4141 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4201 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4261 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4321 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4381 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4441 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4501 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4561 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4621 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4681 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4741 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4801 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4861 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4921 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4981 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5041 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5101 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5161 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5341 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5401 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5461 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5521 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5581 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5641 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5701 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5761 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5821 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5881 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5941 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6001 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6061 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6121 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     6181 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     6241 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     6301 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     6361 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     6421 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     6481 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     6541 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6721 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     6781 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     6841 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     6901 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     6961 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     7021 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     7081 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     7141 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     7201 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     7261 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     7321 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     7381 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     7441 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     7501 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7561 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7621 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7681 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     7741 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     7801 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     7861 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     7921 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     7981 tcaccattat cgtttcagac ccacctccca accccgaggg gacccttgcg ccttttccaa
     8041 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     8101 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     8161 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     8221 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     8281 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     8341 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     8401 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     8461 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     8521 ccccaggggg taccaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     8581 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     8641 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     8701 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     8761 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     8821 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     8881 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggcctgcagc
     8941 tgcagtagta agaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     9001 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9061 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9121 acagggacag cagagatcca ctttggcgcc ggctcgaggg gg
//
