LOCUS V034955 11003 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V034955 VERSION V034955 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers polyA_signal 216..350 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" promoter 398..502 /gene="bla" /label="AmpR promoter" CDS 503..1363 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 1534..2119 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" protein_bind 2407..2428 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 2443..2473 /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2481..2497 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" primer_bind 2505..2521 /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" LTR 2650..3283 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 3490..4078 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" CDS complement(4092..4691) /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" promoter complement(4700..5029) /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS complement(5040..5786) /codon_start=1 /product="modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline" /transl_table=1 /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG*" /label="Tet-On® 3G" promoter complement(5805..6315) /note="human phosphoglycerate kinase 1 promoter" /label="hPGK promoter" promoter 6332..6699 /note="3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G, modified to eliminate binding sites for endogenous mammalian transcription factors" /label="TRE3GS promoter" CDS 6712..8385 /label="GLUD1(NM_005271)" /note="GLUD1(NM_005271)" /gene="GLUD1" CDS 8392..8457 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" CDS 8467..8496 /codon_start=1 /product="10xHis affinity tag" /transl_table=1 /translation="HHHHHHHHHH" /label="10xHis" polyA_signal 8683..8817 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" misc_feature 8861..8976 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" CDS complement(9239..9283) /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 9468..9701 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" misc_feature 10198..10323 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" LTR 10370..11003 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" ORIGIN 1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac catgtaccgc 6721 tacctgggcg aagcgctgtt gctgtcccgg gccgggcccg ctgccctggg ctcggcgtcc 6781 gccgactcgg ccgcgttgct gggctgggcc cggggacagc ccgccgccgc cccgcagccg 6841 gggctggcat tggccgcccg gcgccactac agcgaggcgg tggccgaccg cgaggacgac 6901 cccaacttct tcaagatggt ggagggcttc ttcgatcgcg gcgccagcat cgtggaggac 6961 aagctggtgg aggacctgag gacccgggag agcgaggagc agaagcggaa ccgggtgcgc 7021 ggcatcctgc ggatcatcaa gccctgcaac catgtgctga gtctctcctt ccccatccgg 7081 cgcgacgacg gctcctggga ggtcatcgaa ggctaccggg cccagcacag ccagcaccgc 7141 acgccctgca agggaggtat ccgttacagc actgatgtga gtgtagatga agtaaaagct 7201 ttggcttctc tgatgacata caagtgtgca gtggttgatg tgccgtttgg gggtgctaaa 7261 gctggtgtta agatcaatcc caagaactat actgataatg aattggaaaa gatcacaagg 7321 aggttcacca tggagctagc aaaaaagggc tttattggtc ctggcattga tgtgcctgct 7381 ccagacatga gcacaggtga gcgggagatg tcctggatcg ctgataccta tgccagcacc 7441 atagggcact atgatattaa tgcacacgcc tgtgttactg gtaaacccat cagccaaggg 7501 ggaatccatg gacgcatctc tgctactggc cgtggtgtct tccatgggat tgaaaatttc 7561 atcaatgaag cttcttacat gagcatttta ggaatgacac cagggtttgg agataaaaca 7621 tttgttgttc agggatttgg taatgtgggc ctacactcta tgagatattt acatcgtttt 7681 ggtgctaaat gtattgctgt tggtgagtct gatgggagta tatggaatcc agatggtatt 7741 gacccaaagg aactggaaga cttcaaattg caacatgggt ccattctggg cttccccaag 7801 gcaaagccct atgaaggaag catcttggag gccgactgtg acatactgat cccagctgcc 7861 agtgagaagc agttgaccaa atccaacgca cccagagtca aagccaagat cattgctgaa 7921 ggtgccaatg ggccaacaac tccagaagct gacaagatct tcctggagag aaacattatg 7981 gttattccag atctctactt gaatgctgga ggagtgacag tatcttactt tgagtggctg 8041 aagaatctaa atcatgtcag ctatggccgt ttgaccttca aatatgaaag ggattctaac 8101 taccacttgc tcatgtctgt tcaagagagt ttagaaagaa aatttggaaa gcatggtgga 8161 actattccca ttgtacccac ggcagagttc caagacagga tatcgggtgc atctgagaaa 8221 gacatcgtgc actctggctt ggcatacaca atggagcgtt ctgccaggca aattatgcgc 8281 acagccatga agtataacct gggattggac ctgagaacag ctgcctatgt taatgccatt 8341 gagaaagtct tcaaagtgta caatgaagct ggtgtgacct tcacaaccgg tgactacaaa 8401 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagttc 8461 gaacaccatc accatcacca tcaccatcac catcatcact agggatccag accacctccc 8521 ctgcgagcta agctggacag ccaatgacgg gtaagagagt gacatttttc actaacctaa 8581 gacaggaggg ccgtcagagc tactgcctaa tccaaagacg ggtaaaagtg ataaaaatgt 8641 atcactccaa cctaagacag gcgcagcttc cgagggattt gagatccaga catgataaga 8701 tacattgatg agtttggaca aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt 8761 gaaatttgtg atgctattgc cttatttgta accattataa gctgcaataa acaagttaag 8821 tcgataaact ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat 8881 ttgtttttgt agttctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc 8941 tgcactgtac cccccaatcc ccccttttct tttaaaggcg ataccgtcga gatccgttca 9001 ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc 9061 tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc 9121 taactctatt cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta 9181 ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca 9241 aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg 9301 attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca 9361 tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag 9421 gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat 9481 cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg 9541 tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca 9601 ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag 9661 tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt 9721 ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta 9781 ctactttata tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc 9841 tgaagatcag cggccggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc 9901 tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag 9961 ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg 10021 tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc 10081 gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc 10141 ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac 10201 gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac 10261 cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga 10321 gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt 10381 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg 10441 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct 10501 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca 10561 aaaagcagct gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc 10621 acgcctccct ggaaagtccc cagcggaaag tcccttgtag caagctcgat atcagcagtt 10681 cttgaagtac tccggatgca gctctcgggc cacgtgatga aatgctaggc ggctgtcaaa 10741 cctccactct aacacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa 10801 caagctggtg ttctctcctt tattggcctc ttctacctta tctggctcaa ctggtactag 10861 cttgtagcac catccaaagg tcagtggata tctgacccct ggccctggtg tgtagttctg 10921 ctaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttct 10981 ttgggagtga attagccctt cca //