LOCUS V038500 10666 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038500 VERSION V038500 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..3559 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 3629..5434 /label="Zmynd11(NM_001347474)" /note="Zmynd11(NM_001347474)" /gene="Zmynd11" promoter 5454..5953 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5974..6573 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6587..7175 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7382..8015 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8144..8160) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8168..8184 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8192..8222) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8237..8258 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8546..9134) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9305..10165) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10166..10270) /gene="bla" /label="AmpR promoter" polyA_signal 10318..10452 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 2881 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 2941 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 3001 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 3061 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 3121 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 3181 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 3241 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 3301 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3361 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3421 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 3481 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 3541 catggacgag ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg 3601 gggaagcgga ggcggaggga gtggatccat ggcacgttta acaaaaagac gacaggcgga 3661 tacaaaagct atccagcatc tttgggcagc cattgaaatt atcagaaatc agaagcagat 3721 tgccaacatt gatcgtatta caaagtatat gtcccgagtc cacggtatgc atcccaaaga 3781 gaccacccgc cagctgagct tagctgtgaa agatggtctt attgtcgaaa ctctaacagt 3841 gggctgcaaa ggttcaaaag ctggtattga acaggaagga tattggttac caggagatga 3901 gattgactgg gaaacagaaa ctcatgactg gtattgtttt gaatgccatt tgcctggaga 3961 ggtgttgata tgtgacctgt gttttcgtgt gtatcattcc aagtgtttgt ctgatgagtt 4021 caggcttaga gacagcagta gtcactggca gtgcccagtt tgcaggagca ttaagaagaa 4081 gcactctaac aagcaggaaa tgggcaccta cttgaggttc atcgtctccc gaatgaagga 4141 gcgggctata gaccttaata agaaaggaaa ggacagtaag catcctatgt accggaggct 4201 ggtccactca gctgttgacg tccctaccat acaagagaag gtgaatgagg ggaagtaccg 4261 gagttacgag gagttcaagg ctgacgcaca gctgcttctc cacaacacag tgatcttcta 4321 tggagcagac agtgagcagg cggacattgc acggatgtta tataaagaca cgtgtcacga 4381 gctggacgag ctgcagcttt gcaagaactg cttctatctg tcaaacgcac ggcccgacaa 4441 ctggttctgc tacccttgca tacctaatca tgagctggtt tgggctaaaa tgaaaggttt 4501 tgggttttgg ccagccaaag tcatgcagaa agaagacaat caagtggatg ttcgcttctt 4561 tggccatcac caccagagag cctggattcc ttctgaaaac atccaggata tcacggtcaa 4621 tgttcaccga ctgcacgtga agcgcagcat gggctggaag aaggcctgtg acgagctgga 4681 actgcatcag cgcttcctcc gtgaaggaag gttctggaag tctaagaatg aggaccgagg 4741 tgaggaagag gcagaatcca gtatctcctc caccagtaac gagcagctga aggtcactca 4801 agagccaaga gcaaagaaag gacgacgtaa tcagagtgtg gagcccaaaa aggaagaacc 4861 cgagccagaa acggaagcag tgagttccag ccaggaaatc cccacaatgc ctcagccaat 4921 tgagagggtg tcagtgtcaa cccagaccaa gaagttaagt gcctcttccc cacgaatgct 4981 gcatcgaagc actcagacga cgagcgacgg cgtctgtcag agcatgtgcc acgacaagta 5041 caccaaaatt tttaatgact ttaaagacag gatgaagtcg gaccacaagc gggaaacaga 5101 gagagtggtc cgtgaagcgc tggaaaagtt gcgttctgaa atggaagaag aaaaaagaca 5161 ggccgtgaac aaagctgtgg ccagcctgca gggcgacatg gacagaaagg gtaagcagct 5221 gaaggagaag tgcaaggaag agtttgtaga ggagatcaag aagctagcgg cgcagcacaa 5281 gcagctcatt tcccagacca agaagaagca gtggtgctac aactgtgagg aggaggccat 5341 gtaccactgc tgctggaaca cgtcctactg ctccatcaag tgccagcagg agcactggca 5401 cgcagagcac aagcgcacct gccgccggaa gagatgatct agataattct accgggtagg 5461 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 5521 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 5581 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 5641 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 5701 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 5761 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 5821 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 5881 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 5941 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc 6001 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 6061 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 6121 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 6181 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 6241 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 6301 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 6361 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 6421 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 6481 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 6541 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga 6601 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 6661 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 6721 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 6781 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 6841 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 6901 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 6961 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 7021 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 7081 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 7141 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 7201 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 7261 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 7321 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 7381 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 7441 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 7501 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 7561 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 7621 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 7681 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 7741 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 7801 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 7861 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 7921 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 7981 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 8041 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 8101 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 8161 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 8221 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 8281 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 8341 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 8401 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 8461 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 8521 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 8581 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 8641 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 8701 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 8761 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 8821 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 8881 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 8941 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 9001 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 9061 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 9121 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 9181 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 9241 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 9301 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 9361 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 9421 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 9481 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 9541 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 9601 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 9661 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 9721 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 9781 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 9841 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 9901 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 9961 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 10021 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 10081 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 10141 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 10201 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 10261 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac 10321 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 10381 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 10441 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 10501 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 10561 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt 10621 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt //