LOCUS       V038568                10072 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038568
VERSION     V038568
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..4741
                     /label="XRCC1(NM_006297)"
                     /note="XRCC1(NM_006297)"
                     /gene="XRCC1"
     CDS             4748..4837
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4860..5359
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5380..5979
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5993..6581
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6788..7421
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7550..7566)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7574..7590
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7598..7628)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7643..7664
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7952..8540)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8711..9571)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9572..9676)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9724..9858
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgccgga gatccgcctc cgccatgtcg tgtcctgcag
     2881 cagccaggac tcgactcact gtgcagaaaa tcttctcaag gcagacactt accgaaaatg
     2941 gcgggcagcc aaggcaggcg agaagaccat ctctgtggtc ctacagttgg agaaggagga
     3001 gcagatacac agtgtggaca ttgggaatga tggctcagct ttcgtggagg tgctggtggg
     3061 cagttcagct ggaggcgctg gggagcaaga ctatgaggtc cttctggtca cctcatcttt
     3121 catgtcccct tccgagagcc gcagtggctc aaaccccaac cgcgttcgca tgtttgggcc
     3181 tgacaagctg gtccgggcag ccgccgagaa gcgctgggac cgggtcaaaa ttgtttgcag
     3241 ccagccctac agcaaggact ccccctttgg cttgagtttt gtacggtttc atagcccccc
     3301 agacaaagat gaggcagagg ccccgtccca gaaggtgaca gtgaccaagc ttggccagtt
     3361 ccgtgtgaag gaggaggatg agagcgccaa ctctctgagg ccgggggctc tcttcttcag
     3421 ccggatcaac aagacatccc cagtcacagc cagcgaccca gcaggaccta gctatgcagc
     3481 tgctaccctc caggcttcta gtgctgcctc ctcagcctct ccagtctcca gggccatagg
     3541 cagcacctcc aagccccagg agtctcccaa agggaagagg aagttggatt tgaaccaaga
     3601 agaaaagaag acccccagca aaccaccagc ccagctgtcg ccatctgttc ccaagagacc
     3661 taaattgcca gctccaactc gtaccccagc cacagcccca gtccctgccc gagcacaggg
     3721 ggcagtgaca ggcaaacccc gaggagaagg caccgagccc agacgacccc gagctggccc
     3781 agaggagctg gggaagatcc ttcagggtgt ggtagtggtg ctgagtggct tccagaaccc
     3841 cttccgctcc gagctgcgag ataaggccct agagcttggg gccaagtatc ggccagactg
     3901 gacccgggac agcacgcacc tcatctgtgc ctttgccaac acccccaagt acagccaggt
     3961 cctaggcctg ggaggccgca tcgtgcgtaa ggagtgggtg ctggactgtc accgcatgcg
     4021 tcggcggctg ccctcccaga ggtacctcat ggcagggcca ggttccagca gtgaggagga
     4081 tgaggcctct cacagcggtg gcagcggaga tgaagccccc aagcttcctc agaagcaacc
     4141 ccagaccaaa accaagccca ctcaggcagc tggacccagc tcaccccaga agcccccaac
     4201 ccctgaagag accaaagcag cctcaccagt gctccaggaa gatatagaca ttgagggggt
     4261 acagtcagaa ggacaggaca atggggcgga agattctggg gacacagagg atgagctgag
     4321 gagggtggca gagcagaagg aacacagact gccccctggc caggaggaga atggggaaga
     4381 cccgtatgca ggctccacgg atgagaacac ggacagtgag gaacaccagg agcctcctga
     4441 tctgccagtc cctgagctcc cagatttctt ccagggcaag cacttctttc tttacgggga
     4501 gttccctggg gacgagcggc ggaaactcat ccgatacgtc acagccttca atggggagct
     4561 cgaggactat atgagtgacc gggttcagtt tgtgatcaca gcacaggaat gggatcccag
     4621 ctttgaggag gccctgatgg acaacccctc cctggcattc gttcgtcccc gatggatcta
     4681 cagttgcaat gagaagcaga agttacttcc tcaccagctc tatggggtgg tgccgcaagc
     4741 cggatccgaa caaaaactaa tatcagagga agatttggaa cagaaactaa tcagtgaaga
     4801 agatttagag caaaagttaa tttccgagga ggacttatag tgatctagat aattctaccg
     4861 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     4921 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     4981 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5041 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5101 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5161 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5221 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5281 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5341 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     5401 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5461 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     5521 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     5581 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     5641 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     5701 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     5761 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     5821 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     5881 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     5941 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc
     6001 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6061 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6121 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6181 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6241 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6301 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6361 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     6421 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6481 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6541 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     6601 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6661 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6721 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6781 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     6841 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     6901 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     6961 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     7021 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     7081 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     7141 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     7201 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     7261 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7321 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7381 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7441 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     7501 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7561 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7621 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7681 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7741 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     9721 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9781 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9841 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     9901 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     9961 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    10021 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
