LOCUS       V034044                11411 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034044
VERSION     V034044
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2820..2829
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2835..2924
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     CDS             2931..6179
                     /label="UBE3C(NM_014671)"
                     /note="UBE3C(NM_014671)"
                     /gene="UBE3C"
     promoter        6199..6698
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6719..7318
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7332..7920
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8127..8760
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8889..8905)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8913..8929
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8937..8967)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8982..9003
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9291..9879)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10050..10910)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10911..11015)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11063..11197
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatggc ggcggaacaa aaactaatat cagaggaaga tttggaacag aaactaatca
     2881 gtgaagaaga tttagagcaa aagttaattt ccgaggagga cttaggatcc atgttcagct
     2941 tcgaaggcga cttcaagacg cggcccaagg tgtcccttgg cggcgcgagc aggaaggagg
     3001 aaaaggcttc tcttttacat cgtactcagg aagaaagaag aaagagagag gaagaaaggc
     3061 gaaggttgaa aaatgcaata attatccagt catttattcg aggctataga gacagaaaac
     3121 agcaatattc catccaaaga agtgcatttg atcgctgtgc taccttgtca cagtccgggg
     3181 gcgcttttcc cattgctaat ggccccaacc ttaccctttt ggtaaggcag cttctgtttt
     3241 tttacaaaca aaatgaagac tcaaaacgtt tgatatggct gtatcagaac ttaattaaac
     3301 acagctctct gtttgtcaag cagttggatg gatctgagag acttacatgc ttatttcaga
     3361 taaaaagatt gatgagcctc tgttgcaggt tgctgcaaaa ctgtaatgat gacagtttga
     3421 atgttgcact tccaatgaga atgcttgaag tattttcgtc tgagaatact tacttgcctg
     3481 ttttacaaga tgctagctat gtggtgtcag tgattgaaca aattttgcac tacatgattc
     3541 acaatgggta ttataggtct ctatatttgt tgattaacag caagcttcca tcaagtattg
     3601 aatattctga tttatctcga gttcctatag caaaaatttt gctagagaat gttctaaaac
     3661 cattgcactt tacttacaac tcctgtccgg aaggtgcgag gcaacaagtt tttacagcct
     3721 tcacagagga gtttctggca gcacctttta cagatcagat ttttcatttc atcattccgg
     3781 cgcttgcaga tgcgcagacc gttttccctt acgagccctt tctgaatgca ctgttgttaa
     3841 tagagagtag atgttcaaga aagagtggtg gagcaccctg gcttttctat ttcgttttaa
     3901 ctgttggcga aaattatttg ggggccctct ctgaggaagg gctgctggtg tatttgcggg
     3961 tgctgcagac cttcctctct cagttaccag tctctcctgc cagcgcgagc tgtcacgact
     4021 cagccagtga ctctgaggag gagagtgaag aagccgacaa gccctcaagc ccggaggatg
     4081 gcagactgtc agtatcatac ataacagagg aatgcctgaa gaagctggac acaaagcagc
     4141 agaccaacac cctgctcaac ctggtgtgga gggactctgc gagcgaggag gtcttcacca
     4201 ccatggcctc cgtctgccac acgctgatgg tgcagcaccg catgatggta cccaaagtca
     4261 ggcttctcta cagtttagcc tttaatgcca ggtttctgag acatctttgg tttctaatat
     4321 cttccatgtc aacacggatg atcacagggt ctatggtacc gttgcttcag gtgatatcca
     4381 ggggttctcc tatgtctttt gaagattcta gtcgaatcat cccactcttt tatcttttta
     4441 gctccttgtt tagtcattca ctaatttcca tacatgataa cgaattcttc ggtgatccca
     4501 tagaagttgt aggtcaaaga caatcatcaa tgatgccttt tactttagaa gagctgataa
     4561 tgttgtctcg atgccttcga gatgcatgcc tggggatcat caagttggct tatccagaaa
     4621 ccaagccaga agttcgagaa gaatatatta cagcatttca gagtattgga gttactacta
     4681 gctctgaaat gcaacaatgc atacagatgg aacagaaaag atggattcag ttatttaagg
     4741 ttatcaccaa tctagtgaaa atgttgaagt ccagagacac gaggagaaat ttttgtcctc
     4801 caaaccactg gctgtcagaa caagaagata ttaaagcaga taaggtcact cagctctatg
     4861 tgccagcatc cagacatgtg tggaggttcc ggcggatggg gaggataggc ccgctgcagt
     4921 ccaccctgga cgtgggtttg gagtccccgc cgctgtctgt gtctgaggaa agacagcttg
     4981 ctgtcctgac agagttgcct tttgtggttc catttgagga acgagtaaag atctttcaga
     5041 ggttgattta tgcagataag caagaagttc aaggagatgg tccatttctg gatggaatta
     5101 atgtcacaat aagaagaaat tacatttatg aagatgctta tgacaaactt tctccagaaa
     5161 atgagcctga tttgaaaaag cggatccgtg tgcacttgct caatgcccat ggcctggatg
     5221 aagctggcat tgatggtggt ggtattttca gagagttttt aaatgaacta ctgaagtcag
     5281 gatttaaccc caaccagggg ttctttaaga ctactaatga agggcttctg taccccaacc
     5341 cggctgctca gatgcttgtg ggagattctt ttgccagaca ttactacttc ctaggcagaa
     5401 tgcttggaaa ggctctctat gagaacatgc tggtggagct gccctttgca ggcttctttc
     5461 tttccaagtt gcttggaacc agtgccgacg tggacattca ccacctcgcc tccctagacc
     5521 ctgaggtgta taagaatttg ctctttctga agagctacga agacgatgtg gaggagcttg
     5581 ggctgaactt cactgtggtg aacaatgacc tgggagaggc gcaggtagtt gaactaaaat
     5641 tcggtgggaa agacatccct gtcaccagcg ccaaccggat tgcgtacatc cacttggtgg
     5701 cagactacag gctgaacagg cagatccgcc agcactgcct ggctttccgc cagggccttg
     5761 ccaatgtcgt cagcctcgag tggctccgaa tgtttgatca gcaagaaatt caggtattaa
     5821 tttctggtgc acaagttccc ataagcctag aggacctaaa atcctttaca aactattcag
     5881 gaggctattc tgcagaccat cctgttatta aggtcttctg gagagttgtg gaagggttca
     5941 ctgatgaaga aaagcgcaaa ctgctgaagt ttgtaacaag ctgctctcga ccccctctct
     6001 tggggtttaa ggagttgtat cccgcatttt gtattcacaa cggaggctcc gaccttgagc
     6061 ggctccccac agccagcacc tgcatgaacc tgctgaagct ccccgagttc tatgacgaga
     6121 cacttttgcg aagtaaactt ctctatgcga ttgaatgtgc cgctggcttt gagctgagct
     6181 aggaattcta attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     6241 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     6301 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     6361 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     6421 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     6481 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     6541 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     6601 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     6661 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     6721 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6781 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6841 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6901 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6961 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     7021 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     7081 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     7141 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     7201 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     7261 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc
     7321 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7381 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7441 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7501 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7561 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7621 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7681 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     7741 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7801 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7861 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7921 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     7981 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     8041 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8101 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     8161 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     8221 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     8281 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     8341 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     8401 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     8461 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     8521 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     8581 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8641 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8701 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8761 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     8821 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg
     8881 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8941 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9001 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9061 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9121 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9181 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9241 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9721 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9901 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9961 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10021 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10081 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10141 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10201 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10261 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10321 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10381 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10441 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10501 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10561 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10621 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10681 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10741 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10801 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10861 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10921 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10981 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11041 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
    11101 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11161 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    11221 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    11281 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    11341 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca
    11401 aacttagtag t
//
